Arhgap24 (NM_001012032) Rat Tagged ORF Clone

CAT#: RR211687

Arhgap24 (Myc-DDK-tagged ORF) - Rat Rho GTPase activating protein 24 (Arhgap24), (10 ug)

Product Images

Circular map for RR211687

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgap24
• Synonyms: DR-NR#2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211687 representing NM_001012032
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAAGACCGAAATTCGGGGGGACGCCCTTCAGGTGCCTTAGCATCAACTCCTTTCATCCCCAAAA
  CTACATACAGAAGAATCAAACGGTGCTTTAGTTTCCGGAAAGGTATTTTTGGACAGAAACTGGAGGACAC
  GGTCCGATATGAGAAGAGATACGGGAACCGGCTGGCGCCCATGCTGGTGGAGCAGTGCGTGGACTTCATC
  CGACAGCGGGGGCTGAAGGAAGAGGGCCTCTTCCGACTGCCCGGCCAGGCAAACCTTGTCAAGGAGCTCC
  AGGATGCATTCGACTGTGGAGAGAAGCCCTCCTTCGACAGCAACACAGACGTCCACACTGTGGCATCCCT
  CCTGAAACTGTATCTCCGAGAACTTCCAGAGCCGGTCGTTCCTTACGCAAAGTACGAAGACTTTTTGTCC
  TGTGCCACGCTGCTCAGCAAGGAGGAAGAAGCTGGCGTGAAGGAGCTAACGAAACAAGTGAAGAGTTTGC
  CAGTGGTTAACTACAACCTCCTGAAGTACATTTGCAGGTTCCTGGATGAAGTGCAGTCCTATTCGGGAGT
  TAACAAGATGAGCGCACAGAACTTGGCAACTGTCTTCGGTCCTAACATCCTGCGCCCAAAGGTGGAAGAT
  CCTTTAACCATCATGGAGGGCACTGTGGTGGTCCAGCAGTTAATGTCAGTGATGATCAGCAAACATGATC
  GCCTCTTCCCCAAAGACACAGAGCCACAAAGCAAGCCCCAAGAAGGACCGAACAGCAATAACAACGATGG
  TCATAAGAAAGTCACCATGGGTCAGTTACAGAACAAAGAAAACAACAATACCAAGGAGAGTCCGGTCAGA
  CGGTGCTCTTGGGACAAGCCTGAATCCCCCCAGAGAAGCAGCATGGACAACGGATCCCCCACAGCTCTAT
  CAGGGAGCAAAACCAACAGCCCACGGAACAGTATCCACAAGCTGGATGTCTCCAGGAGCCCGCCTCTCAC
  GGTCAAAAAGAACCCAGCCTTCAATAAGGGCAGCGGGATCGTCACCAATGGGTCCTTCAGCAGCAGCAAT
  GCAGAAGGTGTGGAGAAAACCCAAACCACTCCTAATGGGAGTTTACAAGCCAGAAGGACCTCTTCCCTGA
  AGAGTTCTGGTACCAAAATGGGTACTCATAGCGTACAGAATGGCACCGTGCGCATGGGTATTTTGAACAC
  TGACACACTAGGGAATTCCTTGAATGGCCGGAGCATGAGTTGGCTGCCCAATGGCTACGTGACCCTGAGA
  GATAACAAGCAGAAGGAGCCCGCGGGAGAGTCAGGCCAGCACAACAGACTCTCCACCTACGACAACGTCC
  ACCAGCAGTTCTCCTTGATGAACCTCGACGACAAGCACAGCGTGGACAGTGCCACGTGGTCCACCTCCTC
  CTGTGAAATCTCCCTCCCCGAGAACTCCAACTCCTGCCGCTCCTCTACCACTACGTGCCCGGAGCAAGAC
  TTCTACGGCGGGAACTTTGAGGACCCTGTTTTAGACGGCCCGCCTCAGGATGACCTTTCTCATCCTGGGG
  ACTATGAGAACAAAAGCGACCGCAGGAGCGTGGGGGGCCGAAGCAGCCGTGCCACGAGCAGCAGCGACAA
  CAGCGAGACGTTCGTCGGTAACACCAGCAGCAACCACAGTGCACTTCACAGTTTAGTCTCCAGCCTGAAG
  CAGGAGATGACCAAACAGAAGATCGAGTATGAGTCCAGGATAAAAAGCTTAGAACAGCGGAACTTGACTT
  TGGAAACGGAAATGCTGAACCTCCATGACGAACTGGATCAAGAGAGGAAAAAGTTCACCATGATAGAAAT
  CAAGATGAGAAACGCCGAGCGCGCGAAAGAGGATGCTGAGAAGAGGAACGACATGCTCCAGAAGGAAATG
  GAGCAGTTCTTCTCCACATTCGGAGACCTGACGGTGGAACCCAGGAGAAGCGAGAGGGGAAACACAATCT
  GGATCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211687 representing NM_001012032
  Red=Cloning site Green=Tags(s)
  
  MPEDRNSGGRPSGALASTPFIPKTTYRRIKRCFSFRKGIFGQKLEDTVRYEKRYGNRLAPMLVEQCVDFI
  RQRGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKLYLRELPEPVVPYAKYEDFLS
  CATLLSKEEEAGVKELTKQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSAQNLATVFGPNILRPKVED
  PLTIMEGTVVVQQLMSVMISKHDRLFPKDTEPQSKPQEGPNSNNNDGHKKVTMGQLQNKENNNTKESPVR
  RCSWDKPESPQRSSMDNGSPTALSGSKTNSPRNSIHKLDVSRSPPLTVKKNPAFNKGSGIVTNGSFSSSN
  AEGVEKTQTTPNGSLQARRTSSLKSSGTKMGTHSVQNGTVRMGILNTDTLGNSLNGRSMSWLPNGYVTLR
  DNKQKEPAGESGQHNRLSTYDNVHQQFSLMNLDDKHSVDSATWSTSSCEISLPENSNSCRSSTTTCPEQD
  FYGGNFEDPVLDGPPQDDLSHPGDYENKSDRRSVGGRSSRATSSSDNSETFVGNTSSNHSALHSLVSSLK
  QEMTKQKIEYESRIKSLEQRNLTLETEMLNLHDELDQERKKFTMIEIKMRNAERAKEDAEKRNDMLQKEM
  EQFFSTFGDLTVEPRRSERGNTIWIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012032
• ORF Size: 1968 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012032.1, NP_001012032.1
• RefSeq Size: 2728 bp
• RefSeq ORF: 1971 bp
• Locus ID: 305156
• UniProt ID: Q5U2Z7
• Cytogenetics: 14p22
• MW: 73.5 kDa
• Gene Summary: Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis (By similarity).[UniProtKB/Swiss-Prot Function]