Myh3 (NM_012604) Rat Untagged Clone

CAT#: RN211231

Myh3 (untagged ORF) - Rat myosin, heavy chain 3, skeletal muscle, embryonic (Myh3), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Myh3
• Synonyms: Myhse; RNMHCG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211231 representing NM_012604
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAGCGACACCGAGATGGAAGTGTTTGGCATAGCTGCCCCTTTCCTCCGAAAGTCGGAAAAGGAGA
  GGATCGAAGCTCAGAACCAGCCCTTTGATGCCAAAACCTATTGCTTTGTGGTTGACTCAAAGGAAGAATA
  TGCCAAGGGGAAAATTAAGAGTAGCCAGGATGGGAAAGTCACGGTGGAGACAGAAGACAACAGGACCCTG
  GTGGTTAAACCAGAGGACGTGTATGCCATGAACCCTCCCAAGTTTGACAAGATCGAGGACATGGCCATGC
  TGACCCACCTGAACGAGCCTGCTGTGCTGTACAACCTCAAGGACCGATACACGTCCTGGATGATCTACAC
  CTATTCAGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGTTGCCGGTGTACACCCCTGAGGTGGTG
  GATGGCTACCGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGACAACGCCTACC
  AGTTCATGCTGACGGATCGTGAGAACCAGTCCATCCTGATCACCGGAGAATCCGGGGCCGGGAAGACGGT
  GAACACCAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGCCACTGGGGACCTTGCCAAGAAGAAGGAC
  TCCAAAATGAAGGGGACCCTGGAAGATCAAATCATCAGCGCCAACCCCCTGCTGGAGGCCTTTGGGAATG
  CCAAGACCGTGAGGAATGACAACTCTTCCCGCTTTGGCAAGTTCATCCGCATCCATTTCGGCACCACTGG
  GAAGCTGGCCTCTGCAGATATTGAAACTTATCTGCTGGAAAAGTCCAGAGTCACCTTCCAGCTGAAGGCT
  GAGAGAAGCTATCACATCTTCTACCAGATTCTTTCCAACAAGAAGCCGGAACTGATCGAGTTGCTGCTGA
  TTACGACCAACCCTTACGACTACCCATTCATCAGCCAGGGTGAAATCCTGGTGGCCAGCATCGATGATCG
  TGAGGAACTGTTGGCTACAGACAGTGCCATTGACATCCTGGGCTTCACCCCGGAGGAGAAATCTGGACTC
  TATAAACTGACAGGGGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAGAAGCAGCGAGAGGAGCAAG
  CTGAGCCAGATGGCACCGAAGTGGCTGACAAAACAGCCTACTTGATGGGCCTGAACTCTTCAGACCTCCT
  CAAAGCCTTGTGCTTTCCCAGAGTGAAAGTTGGGAATGAGTACGTTACAAAAGGCCAAACTGTGGACCAG
  GTTCACCATGCTGTGAATGCTCTCTCCAAGTCCGTTTATGAGAAGCTGTTCTTGTGGATGGTCACTCGTA
  TTAACCAGCAGCTGGACACAAAGCTACCAAGACAACACTTCATTGGGGTTTTGGACATTGCGGGTTTTGA
  AATTTTTGAGTATAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAGTTTTTC
  AACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACCTTCATCGACT
  TCGGGATGGACCTGGCGGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCTGGAAGA
  GGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCACCTTGGAAAA
  TCCAACAACTTCCAGAAGCCTAAGGTGGTCAAAGGCAAGGCCGAGGCCCACTTCTCCCTCATCCACTATG
  CGGGCACCGTGGACTACAGTGTCTCGGGCTGGCTGGAGAAGAACAAGGACCCCCTGAATGAGACCGTGGT
  GGGGCTGTACCAGAAGTCTTCCAACAGACTCCTGGCCCATCTCTATGCCACCTTCGCTACGACAGATGCT
  GACGGTGGGAAGAAGAAAGTCGCTAAGAAGAAAGGCTCTTCCTTCCAAACAGTGTCTGCGCTGTTCAGGG
  AAAACTTGAACAAGCTGATGTCCAATTTAAGAACCACTCACCCACACTTTGTGCGCTGTATAATACCCAA
  TGAAACCAAGACCCCAGGGGCCATGGAACACAGCCTGGTCCTGCACCAGCTGAGGTGTAACGGGGTGCTA
  GAAGGCATCCGCATCTGCAGGAAGGGGTTCCCCAACAGGATTCTGTATGGAGACTTTAAACAGAGATACC
  GAGTGCTGAACGCCAGTGCCATCCCGGAGGGCCAGTTCATTGACAGCAAGAAAGCTTGCGAGAAGCTCTT
  GGCTTCCATTGACATCGACCACACACAGTACAAATTTGGACACACCAAGGTGTTCTTTAAGGCTGGCTTG
  CTGGGAACCCTGGAGGAGATGCGGGATGAGCGTCTGGCCAAACTGATCACTCGGACACAAGCCGTGTGCA
  GAGGGTTCCTCATGCGTGTGGAATTCCAGAAGATGATGCAGAGGAGGGAATCCATCTTCTGCATCCAGTA
  CAACATCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAGCCCCTG
  CTGAAGAGCGCCGAGACGGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCCAGAAGACCAAGGACGAGC
  TCGCCAAGTCGGAGGCAAAGAGGAAGGAGCTGGAGGAGAAGCTGGTCACTCTGGTACAAGAGAAGAACGA
  CCTGCAGCTCCAAGTACAGGCGGAGAGTGAGAATTTGCTGGATGCCGAGGAGCGATGCGACCAGCTGATC
  AAAGCCAAGTTCCAGCTGGAGGCCAAAATTAAAGAGGTGACCGAAAGAGCTGAGGATGAGGAGGAGATCA
  ACGCCGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCGGAGCTGAAGAAGGACATCGATGA
  CCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAACAAGGTAAAAAACCTTACC
  GAAGAACTGGCAGGCCTGGATGAAACTATTGCAAAGCTAACACGGGAGAAGAAGGCCCTCCAAGAGGCCC
  ACCAGCAGACCCTGGACGACCTCCAGGCTGAGGAAGACAAGGTCAATTCTCTGAGCAAGCTCAAGAGCAA
  GCTGGAGCAGCAAGTGGATGATTTGGAGAGCTCCCTCGAACAAGAGAAGAAGCTGCGTGTGGACCTGGAA
  AGGAACAAGAGGAAGCTGGAGGGAGACCTGAAGCTTGCCCAAGAGTCCATATTAGATCTGGAGAACGACA
  AGCAACAGCTGGACGAGAGGCTCAAGAAGAAGGACTTTGAATATAGCCAGCTGCAGAGCAAGGTGGAAGA
  TGAGCAGACGCTGAGCCTCCAACTTCAGAAGAAAATCAAAGAGTTACAGGCCCGCATTGAGGAGCTGGAG
  GAAGAGATAGAGGCGGAGAGGGCCACCCGGGCCAAGACAGAGAAGCAGCGCAGCGACTACGCCCGCGAGC
  TGGAGGAGCTGAGTGAACGTCTGGAGGAGGCGGGCGGTGTCACCTCTACCCAGATTGAGCTGAACAAGAA
  GCGTGAGGCTGAGTTCTTGAAGCTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAGGCCACCGTG
  GCCACGCTGCGGAAGAAGCACGCGGACAGCGCGGCGGAGCTGGCAGAGCAGATCGACAACCTGCAGCGCG
  TCAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGATCGACGACCTCTCCAGCAGTGTGGA
  GAGTGTGTCCAAATCCAAGGCCAACCTGGAGAAGATATGCCGAACTCTAGAGGATCAGTTAAGCGAGGCC
  AGGGGCAAGAACGAGGAGACGCAGAGAAGCCTAAGTGAGCTGACCACACAGAAGTCCCGGCTGCAGACAG
  AAGCGGGTGAGCTGAGTCGTCAACTGGAAGAAAAGGAAAGCATAGTATCTCAGCTTTCCAGGAGCAAGCA
  AGCCTTTACCCAACAGATAGAGGAGCTCAAGAGGCAACTGGAGGAAGAGAACAAGGCCAAGAATGCGCTG
  GCCCACGCCCTGCAGTCCTCCCGCCACGACTGTGACCTGCTTCGGGAACAGTACGAGGAGGAGCAGGAAG
  GCAAAGCCGAGCTGCAGAGGGCGCTGTCCAAGGCCAACAGCGAGGTGGCCCAATGGAGGACCAAATATGA
  GACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAGGCCAAAAAAAAGCTTGCTCAGCGCCTTCAGGAT
  TCGGAGGAGCAGGTCGAGGCTGTGAATGCAAAATGCGCTTCCTTGGAAAAGACCAAGCAGAGGCTGCAAG
  GGGAGGTGGAGGACTTGATGGTGGATGTGGAGAGAGCCAACTCTCTGGCTGCCGCTCTGGACAAGAAGCA
  GAGGAACTTTGACAAGGTGTTAGCCGAGTGGAAGACGAAGTGTGAGGAGAGCCAAGCAGAGCTGGAGGCG
  GCTCTCAAGGAATCCCGGTCCTTGAGCACTGAGCTCTTCAAACTGAAAAACGCCTATGAAGAAGCCTTGG
  ATCAACTCGAAACTGTGAAGCGAGAGAATAAGAACTTAGAACAAGAGATAGCAGATCTCACCGAACAAAT
  TGCTGAGAACGGTAAAAGCATCCACGAGCTAGAGAAATCCAGAAAGCAGATGGAGCTGGAGAAGGCTGAT
  ATCCAGATGGCGCTGGAGGAAGCAGAGGCAGCTCTGGAGCACGAAGAAGCCAAGATCCTCAGGATCCAGC
  TTGAACTGACGCAGGTGAAATCAGAGATCGATAGAAAGATCGCAGAGAAGGATGAAGAGATCGAGCAGCT
  GAAGAGGAACTACCAGAGGACAGTGGAGACCATGCAGGGCGCCCTGGATGCTGAGGTGCGCAGCAGGAAT
  GAGGCGATCCGGCTGAAGAAGAAGATGGAGGGCGACCTCAATGAGATTGAGATCCAGCTGAGCCATGCCA
  ACCGCCAGGCCGCAGAGACCATCAAGCACCTCCGGAGTGTCCAGGGACAGCTGAAGGACACCCAGCTGCA
  CCTGGATGACGCTCTCCGAGGCCAGGAGGACCTGAAGGAGCAGCTGGCGATCGTGGAGCGCAGAGCCAAC
  CTGCTGCAGGCTGAGGTGGAGGAGCTGCGGGCCACTCTGGAGCAGACGGAGAGGGCCCGGAAGCTGGCAG
  AGCAGGAGCTGCTGGACTCCAACGAGAGGGTTCAGCTGCTGCACACCCAGAACACCAGCCTCATCCACAC
  CAAGAAGAAGTTGGAGACTGACCTCACACAGCTCCAGAGTGAGGTGGAGGATGCCAGCAGGGACGCAAGG
  AACGCCGAGGAGAAGGCCAAAAAGGCCATCACTGATGCCGCCATGATGGCGGAGGAGCTAAAGAAGGAGC
  AGGACACCAGCGCCCATCTGGAGCGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCTGCAGCACCG
  TCTGGACGAGGCTGAGCAGCTGGCACTGAAGGGCGGCAAGAAGCAGATCCAGAAACTGGAGACGAGGATC
  AGAGAGCTAGAGTTTGAGCTGGAAGGGGAACAGAAGAGGAACACAGAGTCTGTGAAGGGCCTGAGGAAGT
  ATGAGCGGCGTGTCAAGGAGCTCACCTACCAGAGTGAGGAGGACAGGAAGAATGTGCTGAGATTGCAGGA
  TCTGGTGGATAAACTTCAAGTGAAAGTCAAGTCCTACAAGAGGCAGGCTGAGGAGGCTGATGAACAAGCC
  AATGTTCATCTCACCAAGTTCCGGAAAGCCCAGCATGAGCTAGAGGAGGCCGAGGAACGTGCGGATATTG
  CAGAATCGCAAGTCAATAAACTGCGGGCTAAAACCCGGGACTTCACCTCTAGCCGGATGGTGGTCCATGA
  AAGTGAGGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012604
• Insert Size: 5823 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012604.1, NP_036736.1
• RefSeq Size: 6035 bp
• RefSeq ORF: 5823 bp
• Locus ID: 24583
• UniProt ID: P12847
• Cytogenetics: 10q24
• Gene Summary: heavy chain of myosin; involved in muscle contraction [RGD, Feb 2006]