Hibch (NM_001013112) Rat Untagged Clone

CAT#: RN211069

Hibch (untagged ORF) - Rat 3-hydroxyisobutyryl-Coenzyme A hydrolase (Hibch), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Hibch
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211069 representing NM_001013112
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCAGCAGTTTGTGTGGCGGCTCCAGTCGAGGTTCAGCTCAATCAGAAGAGCCAGTGTTATTCTGC
  AACATCTGAGAATGTCCAAGCACACAGAGACTGCAGAAGTGCTGCTGGAGAGGAGAGGCTGTGCAGGGGT
  CATAACGCTCAACAGACCCAAGTTACTCAACGCTCTGAGTCTGAATATGATCCGGCAGATCTATCCGCAG
  CTAAAGAAGTGGGAACGAGACCCAGATACATTCCTGATCATCATAAAGGGAGCTGGAGGAAAGGCCTTCT
  GTGCTGGAGGGGACATCAAAGCACTCTCAGAAGCTAAAAAAGCAGGGCAGACTTTGAGTCAAGATTTATT
  CAGAGAAGAATATATCCTGAACAATGCCATTGCGTCTTGTCAGAAACCGTATGTTGCCCTTATTGATGGA
  ATCACAATGGGTGGGGGAGTTGGTCTTTCAGTCCATGGTCAATTCCGAGTGGCTACAGAAAGATCTCTCT
  TTGCTATGCCAGAAACGGGAATAGGACTGTTTCCTGATGTGGGCGGAGGTTATTTCTTGCCAAGACTTCA
  AGGAAAACTGGGTTACTTCCTTGCACTGACAGGATTCAGACTAAAAGGAAGAGATGTGCACAGAGCGGGA
  ATCGCCACACACTTTGTAGATTCAGAAAAGTTGCATGTACTGGAGGAGGAGTTGTTAGCCTTAAAGTCCC
  CTTCAGCTGAGGATGTTGCTGGTGTCTTAGAAAGCTATCATGCCAAGTCCAAGATGGGTCAAGACAAGTC
  TATCATATTTGAGGAACACATGGACAAAATAAACAGTTGTTTTTCAGCCAATACTGTGGAACAGATTCTT
  GAAAACTTACGGCAAGATGGTTCACCTTTTGCTATGGAACAGATTAAGTTCTCATTGACAAAGACCAGAC
  TCCAAAATGGAAACCAGCTGATCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013112
• Insert Size: 936 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013112.1, NP_001013130.1
• RefSeq Size: 1738 bp
• RefSeq ORF: 936 bp
• Locus ID: 301384
• UniProt ID: Q5XIE6
• Cytogenetics: 9q22
• Gene Summary: Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (By similarity).[UniProtKB/Swiss-Prot Function]