Dbnl (NM_031352) Rat Untagged Clone

CAT#: RN209574

Dbnl (untagged ORF) - Rat drebrin-like (Dbnl), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dbnl
• Synonyms: abp1; Sh3p7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN209574 representing NM_031352
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGAACCTGAGCCGGAACGGGCCGGCGCTGCAGGAGGCCTATGTGCGGGTGGTCACCGAGAAAT
  CCCCGACCGACTGGGCTCTTTTTACCTATGAAGGCAACAGCAATGACATCCGTGTGGCTGGCACAGGGGA
  GGGAGGCCTGGAGGAGCTGGTGGAGGAGCTCAACAGCGGGAAGGTGATGTACGCCTTCTGCAGGGTGAAG
  GACCCCAACTCTGGCCTGCCCAAGTTTGTCCTCATCAACTGGACAGGCGAGGGTGTGAATGATGTGCGAA
  AAGGAGCATGTGCCAACCACGTCAGCACCATGGCCAACTTCCTAAAGGGTGCCCATGTGACCATCAACGC
  ACGGGCTGAGGAAGATGTGGAGCCTGAGTGCATCATGGAGAAGGTTGCCAAGGCTTCTGGGGCTAATTAC
  AGCTTCCATAAGGAAAGCAGCTGCTTCCAGGATGTGGGGCCTCAGGCCCCAGTGGGCTCTGTGTACCAGA
  AGACCAATGCCGTGTCTGAGATCAAGAGAGTTGGCAAGGATAACTTCTGGGCCAAAGCTGAGAAGGAGGA
  GGAGAACCGCCGCCTGGAGGAGAAGCGGCGGGCTGAAGAGGAGAAGCAGCGGCTGGAGGAGGAGCGGCGG
  GAGCGGGAGCTGCAGGAGGCTGCCCGACGGGAACAACGCTACCAGGAACAGCACAGATCAGCTGGACCCC
  CAAGCAGGACAGGTGAACTGGAGCAAGAGGTGGTTTCAAGGAGCAGACAGGAATGGGAGTCTGCTGGGCA
  GCAGGCCCCACACCCACGGGAGATTTTCAAGCAGAAGGAAAGGGCTATGTCCACCACCTCTGTCTCCAGC
  TCTCAGCCAGGCAAGCTGAGGAGCCCCTTCCTGCAGAAGCAGTTCACTCAACCAGAAGCTTCCTATGGCC
  GAGAGCCCACTTCTCCTGTGTCACGGCCTGCAGCAGGTGTCTGCGAGGAGCTGGCACCCAGCACTCCGCC
  CTCAGCCCAGACAGACGATGAACCTACATATGAAGTGCCCTCCGAGCAGGAGACCCTGTACGAGGAACCA
  CCACCGGTCCAGCAGCCAGGGGCTGGCTCCGGACACATTGACAACTACATGCAGAGCCAGGACCTCAGTG
  GGCAAGGACTGTGCGCTCGGGCCTTGTACGACTACCAGGCAGCTGACGACACGGAGATCTCCTTTGACCC
  TGAGAACCTAATCACAGGCATCGAGGTGATTGACGAAGGCTGGTGGCGTGGCTATGGGCCTGACGGCCAC
  TTTGGCATGTTTCCTGCCAACTATGTGGAGCTCATAGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031352
• Insert Size: 1302 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031352.2, NP_112642.1
• RefSeq Size: 2286 bp
• RefSeq ORF: 1302 bp
• Locus ID: 83527
• UniProt ID: Q9JHL4
• Cytogenetics: 14q21
• Gene Summary: actin-binding protein involved in actin cytoskeleton organization in dendrites [RGD, Feb 2006]
  Transcript Variant: This variant (2) uses an alternate splice site in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.