Htr3b (NM_022189) Rat Untagged Clone

CAT#: RN209204

Htr3b (untagged ORF) - Rat 5-hydroxytryptamine (serotonin) receptor 3b (Htr3b), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Htr3b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN209204 representing NM_022189
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCTTCTGTGGTCCTGCCTCCTGGTGGCTGTCGTAGGTATTCTAGGCACAGCGACACCTCAGCCTG
  GGAATTCCTCTCTGCATCGCCTCACCAGGCAGCTGCTACAGCAGTACCACAAGGAGGTCAGACCAGTTTA
  CAACTGGGCTGAGGCCACCACTGTCTACCTGGACCTTTGCGTCCATGCTGTATTGGATGTGGATGTACAG
  AATCAAAAATTAAAGACAAGCATGTGGTACCGAGAGGTTTGGAATGATGAGTTTCTGTCCTGGAACTCTA
  GTCTGTTCGATGATATTCAAGAGATCTCTCTCCCTCTCAGTGCCATCTGGGCCCCTGATATCATCATTAA
  TGAGTTTGTGGATGTCGAAAGGTCTCCTGACCTGCCCTATGTATACGTGAACTCTTCCGGAACGATTAGA
  AACCACAAGCCCATCCAGGTGGTCTCCGCATGCAGTTTGCAGACATACGCTTTCCCCTTTGATATCCAGA
  ACTGCAGTCTCACCTTCAATAGCATCCTGCATACAGTGGAAGACATAGACCTGGGCTTCCTGAGGAACCA
  AGAAGACATTGAGAACGACAAAAGGTCTTTTCTGAATGACAGCGAGTGGCAGCTTCTTTCTGTGACCTCC
  ACTTACCACATCCGGCAGAGCAGCGCTGGTGACTTCGCACAGATTCGGTTTAATGTAGTGATTCGCAGAT
  GTCCATTGGCCTATGTTGTGAGCCTCTTGATTCCTAGCATCTTCCTGATGCTCGTGGACCTGGGAAGCTT
  CTACTTGCCCCCCAACTGTCGTGCCAGAATTGTGTTCAAGACCAATGTGCTGGTGGGCTATACAGTCTTC
  AGGGTCAACATGTCTGATGAGGTGCCAAGAAGCGCAGGGTGCACATCTCTGATTGGGGTCTTCTTTACCG
  TCTGCATGGCCCTCTTGGTTCTCAGTTTATCCAAATCCATCTTGTTGATCAAATTCCTCTATGAGGAGCG
  GCACAGTGAGCAAGAACGGCCTCTTATGTGCCTTCGAGGGGACTCTGATGCCAATGAGTCTAGATTGTAC
  CTCAGAGCTCCATGTGCTGAGGACACAGAGTCCCCGGTTCGTCAGGAGCACCAGGTCCCATCAGATACTC
  TGAAGGATTTCTGGTTCCAGCTCCAATCCATCAACAACTCTCTCCGAACTCGGGATCAGGTTTACCAGAA
  GGAGGTGGAGTGGCTCGCCATCCTGTGCCACTTTGATCAGCTGCTCTTCCGAATCTACCTTGCCGTGCTG
  GGGCTCTATACTGTCACCTTATGCTCTCTCTGGGCACTGTGGAGCAGGATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022189
• Insert Size: 1314 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022189.1, NP_071525.1
• RefSeq Size: 1346 bp
• RefSeq ORF: 1314 bp
• Locus ID: 58963
• UniProt ID: Q9JJ16
• Cytogenetics: 8q23
• Gene Summary: a serotonin-gated ion channel subunit with pronounced inward rectification of both the whole-cell and single-channel current amplitudes [RGD, Feb 2006]