Tkt (NM_022592) Rat Untagged Clone

CAT#: RN209174

Tkt (untagged ORF) - Rat transketolase (Tkt), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Tkt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN209174 representing NM_022592
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCAGTGAGACAGATCCAGTGCGACCGTGAAGGCAAACGCTTTCCGTTCCTCTCAGCTCCACCAG
  CTTCCACGTCCTCGCCCGACCGCGCCATGGAGGGTTACCATAAGCCAGATCAGCAGAAGCTCCAGGCCCT
  GAAGGACACAGCCAATCGCCTGCGCATCAGCTCCATCCAGGCCACCACCGCGGCAGGCTCGGGACACCCC
  ACATCATGCTGCAGCGCTGCCGAGATCATGGCTGTCCTGTTTTTCCATACCATGCGCTACAAGGCCCTGG
  ATCCCCGAAACCCTCACAATGATCGCTTTGTGCTCTCCAAGGGCCATGCAGCTCCCATCTTATATGCAGT
  CTGGGCTGAAGCTGGCTTCCTGCCTGAGGCAGAGCTGCTGAACCTGAGGAAAATCAGCTCTGACTTGGAT
  GGGCATCCTGTCCCGAAACAAGCCTTCACCGATGTGGCCACTGGCTCCCTGGGCCAGGGGCTGGGAGCTG
  CGTGCGGGATGGCATACACAGGCAAATACTTCGACAAAGCCAGCTACCGAGTCTATTGCATGCTGGGAGA
  CGGGGAGGTGTCCGAGGGCTCCGTTTGGGAGGCCATGGCCTTCGCTGGAATTTACAAGCTGGACAACCTC
  GTTGCCATTTTTGACATCAACCGTCTGGGCCAGAGCGACCCAGCCCCGCTGCAGCACCAAGTGGACGTCT
  ACCAGAAGCGCTGTGAGGCCTTTGGCTGGCACGCCATCATCGTGGATGGGCACAGTGTGGAGGAGCTGTG
  CAAGGCCTTTGGTCAGGCCAAGCACCAACCAACAGCCATCATTGCCAAGACCTTCAAGGGCCGCGGGATC
  ACAGGGATTGAAGACAAGGAGGCGTGGCATGGGAAGCCCCTCCCCAAAAACATGGCTGAGCAGATTATCC
  AGGAGATTTACAGCCAGGTTCAGAGCAAAAAGAAGATCCTCGCCACGCCCCCTCAGGAGGATGCCCCTTC
  CGTGGACATTGCCAACATCCGAATGCCTACCCCACCCAACTACAAAGTGGGGGACAAGATAGCCACACGG
  AAAGCCTATGGATTGGCCCTTGCCAAGCTGGGCCACGCCAGTGACCGCATCATCGCCCTGGATGGAGACA
  CCAAAAATTCCACCTTCTCAGAGCTCTTCAAAAAGGAGCACCCAGACCGTTTCATCGAGTGCTACATTGC
  TGAGCAGAACATGGTGAGCATTGCTGTGGGCTGTGCCACACGTGACAGGACAGTGCCCTTCTGCAGCACT
  TTTGCGGCCTTCTTCACACGCGCCTTCGACCAGATCCGCATGGCCGCCATCTCCGAGAGCAACATCAACC
  TTTGTGGCTCCCACTGCGGCGTGTCCATTGGGGAAGACGGGCCCTCGCAGATGGCCCTGGAAGACCTGGC
  CATGTTTCGGTCGGTCCCTATGTCCACCGTCTTTTACCCAAGTGATGGAGTTGCCACAGAGAAGGCAGTG
  GAATTAGCAGCCAATACAAAGGGCATCTGCTTCATTCGGACCAGCCGCCCAGAAAATGCCATTATCTATA
  GCAACAACGAGGATTTCCAGGTTGGCCAAGCCAAGGTGGTCCTGAAGAGCAAGGACGACCAAGTGACAGT
  GATCGGGGCTGGCGTAACTCTGCACGAGGCTCTGGCTGCTGCAGAGATGTTGAAGAAAGAGAAGATCGGT
  GTCCGTGTACTGGACCCCTTCACCATCAAGCCCCTGGACAAAAAGCTCATTCTCGACTGTGCCAGAGCAA
  CCAAAGGCAGGATCCTCACCGTGGAGGACCACTACTATGAAGGTGGCATAGGCGAGGCAGTATCTGCTGT
  GGTAGTGGGCGAACCTGGAGTCACAGTCACTCGCCTGGCGGTCAGCCAAGTACCACGAAGTGGGAAGCCA
  GCTGAGCTGCTGAAGATGTTTGGTATTGACAAAGACGCCATTGTGCAAGCTGTGAAGGGCCTTGTCACCA
  AGGGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022592
• Insert Size: 1968 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022592.1, NP_072114.1
• RefSeq Size: 2098 bp
• RefSeq ORF: 1968 bp
• Locus ID: 64524
• UniProt ID: P50137
• Cytogenetics: 16p16
• Gene Summary: human homolog is a key enzyme in the pentose-phosphate pathway and may be associated with Wernicke-Korsakoff syndrome [RGD, Feb 2006]