Lta4h (NM_001030031) Rat Untagged Clone

CAT#: RN207685

Lta4h (untagged ORF) - Rat leukotriene A4 hydrolase (Lta4h), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Lta4h
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN207685 representing NM_001030031
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAGGTCGCGGATACTTGTTCCTTGGCTTCTCCAGCCTCGGTCTGCCGAACCCAGCACCTCCATC
  TTCGCTGCAGCGTCGACTTCGCTCGCCGGGCACTGACCGGGACCGCCGCGCTCACGGTCCAGTCACAGGA
  GGATAATCTGCGCAGCCTGACTTTGGATACAAAAGACCTTACGATAGAAAAAGTGGTGATCAACGGACAA
  GAAGTCAAATACACTCTTGGAGAAAGCCAGGGTTACAAAGGATCGCCGATGGAAATCTCCCTTCCCATCG
  CTCTGAGCAAAAATCAAGAGGTCGTTATAGAGATTTCCTTCGAGACATCTCCCAAGTCCTCGGCCCTTCA
  GTGGCTCACTCCCGAGCAGACTTCAGGAAAGCAGCATCCATACCTCTTCAGTCAGTGCCAGGCCATCCAC
  TGCAGGGCAATCCTCCCTTGCCAAGACACTCCTTCTGTGAAGTTAACCTACACCGCAGAGGTATCTGTCC
  CCAAAGAACTGGTGGCTCTCATGAGTGCCATCCGTGATGGAGAAGCCCCTGACCCAGAAGACCCCAGCAG
  GAAGATATACAGATTTAACCAGAGGGTTCCCATACCGTGCTACCTGATTGCTTTAGTCGTTGGCGCTTTA
  GAAAGCAGGCAAATTGGCCCAAGAACTCTGGTGTGGTCTGAGAAAGAGCAGGTGGAGAAGTCTGCTTATG
  AATTCTCTGAGACTGAATCCATGCTTAAAATTGCAGAAGATCTGGGGGGACCCTATGTTTGGGGACAGTA
  CGACCTGTTGGTCCTGCCCCCATCCTTCCCGTACGGAGGCATGGAGAATCCTTGTCTCACATTCGTAACC
  CCTACTCTATTGGCAGGTGACAAGTCACTCTCTAATGTTATTGCACACGAAATATCGCATAGCTGGACAG
  GGAACCTAGTGACTAACAAGACTTGGGATCACTTCTGGTTAAACGAAGGACATACCGTCTATTTAGAGCG
  CCACATTTGTGGGCGGTTGTTTGGGGAAAAATTCAGACATTTCCACGCTTTAGGAGGATGGGGAGAGCTA
  CAGAACACGATAAAGACTTTCGGGGAGTCCCATCCCTTCACCAAGCTCGTGGTCGATCTGAAGGACGTAG
  ACCCCGATGTGGCCTACTCCTCCATTCCCTACGAGAAGGGCTTCGCCTTGCTCTTCTACCTGGAACAACT
  TCTTGGGGGACCGGAGGTGTTTCTAGGATTCCTAAAGGCTTATGTGGAGAAGTTTTCCTACCAGAGCGTA
  ACCACTGATGACTGGAAGAGTTTCCTGTATGCTCACTTTAAAGATAAGGTGGATCTTCTCAATCAAGTTG
  ATTGGAATGCCTGGCTCTATGCTCCTGGCCTGCCTCCTGTCAAACCCAATTACGATGTGACTCTCACGAA
  TGCCTGCATCGCCTTAAGTCAAAGATGGGTCACTGCCAAAGAGGAAGATTTAAATTCATTCAGCATCGAA
  GACCTGAAGGACCTCTCGTCCCATCAGCTGAATGAGTTCCTGGCACAGGTGCTTCAGAAGGCACCTCTTC
  CCTTGGGGCACATAAAGCGAATGCAAGAGGTGTACAACTTCAATGCCATTAACAATTCTGAAATACGATT
  CAGATGGTTACGGCTCTGCATTCAATCGAAGTGGGAAGAAGCAATTCCTTTGGCCCTAAAGATGGCAACT
  GAACAAGGAAGGATGAAATTTACACGACCTCTATTCAAGGACCTCGCTGCCTTCGACAAATCCCACGATC
  AAGCTGTCCGCACCTACCAGGAACATAAAGCCTGTATGCATCCCGTGACCGCCATGCTGGTGGGGAAAGA
  TCTGAAAGTGGATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001030031
• Insert Size: 1836 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030031.1, NP_001025202.1
• RefSeq Size: 2083 bp
• RefSeq ORF: 1836 bp
• Locus ID: 299732
• UniProt ID: Q499P2
• Cytogenetics: 7q13
• Gene Summary: Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities (By similarity). Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the proinflammatory mediator leukotriene B4 (LTB4) (PubMed:1544505). Has also aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides. In addition to its proinflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions (By similarity).[UniProtKB/Swiss-Prot Function]