Atf4 (NM_024403) Rat Untagged Clone

CAT#: RN207657

Atf4 (untagged ORF) - Rat activating transcription factor 4 (tax-responsive enhancer element B67) (Atf4), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Atf4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN207657 representing NM_024403
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAGATGAGCTTCCTGAACAGCGAAGTGTTGGCGGGGGACTTAATGTCCCCCTTCGACCAGTCGG
  GTTTGGGGGCTGAAGAAAGCCTAGGTCTCTTAGATGACTATCTGGAGGTGGCCAAGCACTTCAAACCTCA
  TGGGTTCTCCAGCGACAAGGCGGGCTCCTCAGAATGGCTGGCTATGGATGGGTTGGTCAGTGCCTCAGAC
  ACCGGCAAGGAGGATGCCTTTTCCGGGACAGATTGGATGTTGGAGAAAATGGACCTGAAAGAGTTTGACT
  TCGATGCTCTGTTTCGAATGGATGACCTGGAAACCATGCCAGATGAGCTTTTGGCCACGTTGGATGACAC
  ATGTGATCTTTTTGCCCCTCTAGTCCAAGAGACTAATAAGGAGCCCCCTCAGACAGTGAACCCAATTGGC
  CATCTCCCAGAAAGTGTAATAAAAGTCGACCAGGCTGCCCCCTTTACATTCTTGCAGCCTCTTCCCTGTT
  CCCCAGGGTTTCTGTCTTCCACTCCAGATCATTCCTTTAGTTTAGAGCTGGGAAGTGAGGTTGATATCTC
  TGAAGGAGATAGGAAGCCTGACTCTGCTGCTTATATTACTCTAACCCCTCAGTGTGTAAAGGAGGAAGAC
  ACTCCCTCTGATAGTGACAGTGGCATCTGTATGAGCCCTGAGTCCTACCTGGGCTCTCCCCAACACAGCC
  CTTCCACCTCCAGGGCCCCACCAGACAGTCTGCCTTCTCCAGGTGTTCCTCGTGGTTCTCGACCCAAACC
  TTATGACCCACCTGGAGTTAGTGTGACAGCTAAAGTGAAGACTGAAAAGTTGGATAAGAAGCTGAAAAAG
  ATGGAGCAAAACAAGACAGCAGCTACTAGGTACCGCCAGAAGAAGAGGGCTGAGCAGGAAGCCCTCACTG
  GCGAGTGTAAAGAGCTAGAAAAGAAGAACGAGGCTCTGAAAGAGAAGGCAGATTCTCTCGCCAAAGAGAT
  TCAGTATCTAAAAGACCTGATAGAAGAGGTCCGTAAGGCAAGGGGGAAGAAGAGAGTTCCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_024403
• Insert Size: 1044 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024403.2, NP_077379.1
• RefSeq Size: 1410 bp
• RefSeq ORF: 1044 bp
• Locus ID: 79255
• UniProt ID: Q9ES19
• Cytogenetics: 7q34
• Gene Summary: binds GABA(B) receptors; may play a role in regulation of activity dependent gene expression [RGD, Feb 2006]