Pdgfra (NM_012802) Rat Untagged Clone

CAT#: RN207014

Pdgfra (untagged ORF) - Rat platelet derived growth factor receptor, alpha polypeptide (Pdgfra), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pdgfra
• Synonyms: APDGFR; PDGFACE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN207014 representing NM_012802
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACCTCCCAGGCCTTTCTGGTCCTCAGCTGTCTCCTCACAGGGCCGAGCCTCATCGTCTGCCAGC
  TCTTATTACCCTCCATCCTTCCAAATGAGAATGAGAAGATTGTGCCGCTGAGTTCGTCCTTCTCTCTGAG
  ATGCTTCGGGGAGAGCGAAGTGAGCTGGCAGCACCCCATGTCTGAAGAAGAGGACCCCAACGTGGAAATC
  AGAACCGAGGAGAACAACAGTAGCCTCTTTGTCACGGTGCTGGAAGTGGTCAACGCCTCAGCGGCCCACA
  CCGGATGGTACACTTGCTATTACAACCACACTCAGACAGAAGAGAGTGAGATCGAAGGCAGGCACATTTA
  TATCTATGTACCAGACCCAGACATGGCCTTCGTGCCTCTCGGCATGACGGATTCTTTAGTCATTGTGGAA
  GAGGACGATTCTGCCATCATACCCTGCCGCACCACGGATCCAGACACTGAAGTAACCTTGCACAATAACG
  GGAGGCTGGTGCCTGCCTCCTACGACAGCAGGCAGGGCTTCAACGGAACCTTCAGTGTGGGGCCTTACAT
  CTGTGAGGCCACCGTCAGAGGGAGGACGTTCAAGACCAGCGAGTTTAACGTTTATGCCTTGAAAGCCACG
  TCAGAACTGAATCTGGAGATGGACACACGCCAGACTGTGTATAAGGCAGGAGAAACGATCGTGGTGACCT
  GCGCCGTCTTTAACAATGAGGTGGTTGACCTGCAGTGGACTTACCCTGGAGAGGTGAGAAACAAAGGCAT
  CACCATGCTGGAGGAGATCAAACTCCCGTCCATCAAACTGGTGTACACGCTGACCGTCCCCAAGGCCACG
  GTGAAGGACAGTGGAGATTATGAATGTGCTGCCCGCCAGGCCACGAAAGAGGTCAAGGAAATGAAGACAG
  TCACCATTTCTGTTCACGAGAAAGGCTTCGTCCAGATCAGGCCCACCTTTGGCCATCTGGAAACTGTGAA
  CCTGCATCAGGTCAGAGAGTTCGTGGTGGAGGTGCAGGCCTACCCGACCCCCAGGATATCCTGGCTGAAG
  GACAACTTGACCCTGATTGAGAATCTCACTGAGATCACCACCGATGTGCAGAGGAGCCAGGAGACGAGGT
  ATCAAAGCAAATTAAAGCTGATCCGGGCTAAGGAAGAAGACAGCGGCCATTATACCATTATAGTTCAGAA
  TGACGACGACATGAAGAGCTACACGTTTGAGCTGTCAACTCTAGTTCCTGCCTCCATTCTGGAGCTTGTG
  GATGACCACCATGGCTCTGGCGGGGGACAGACTGTGAGGTGCACGGCTGAAGGCACCCCTCTTCCGAACA
  TCGAGTGGATGATCTGCAAGGATATTAAGAAATGTAATAATGACACTTCATGGACAGTTTTGGCCAGCAA
  CGTCTCGAATATTATCACGGAGTTCCACCAGCGAGGCAGGAGTACAGTGGAGGGACGAGTGTCCTTCGCC
  AAGGTGGAAGAGACCATCGCAGTTCGGTGCTTGGCAAAGAACGACCTTGGCATTGGGAACAGAGAGCTGA
  AGCTGGTGGCTCCCACCCTGCGATCTGAGCTCACGGTGGCGGCCGCGGTGCTGGTGCTGTTGGTGATTGT
  CATTGTCTCTCTCATTGTCCTGGTTGTCATTTGGAAGCAGAAACCACGGTATGAAATTCGATGGAGGGTC
  ATTGAGTCAATCAGCCCTGATGGACATGAGTATATTTATGTGGACCCCATGCAGCTGCCTTACGACTCCA
  GATGGGAGTTCCCGAGAGATGGACTAGTGCTTGGTCGGATCTTGGGATCCGGTGCATTTGGGAAAGTGGT
  TGAAGGCACAGCTTATGGATTAAGCCGGTCCCAGCCTGTAATGAAGGTGGCTGTGAAGATGCTCAAACCC
  ACAGCCAGGTCTAGTGAGAAGCAAGCTCTCATGTCTGAGCTGAAGATAATGACTCACTTGGGACCGCATC
  TGAACATTGTGAATTTGCTGGGAGCCTGCACCAAGTCAGGCCCCATTTACATCATCACAGAATACTGCTT
  CTATGGGGATTTGGTCAATTACCTGCATAAGAACAGAGATAGCTTCATGAGCCGACACCCAGAGAAGCCA
  AAGAAAGACCTGGACATCTTTGGATTGAATCCTGCAGATGAGAGTACCAGGAGTTACGTGATTTTATCTT
  TTGAAAACAATGGTGACTACGTGGACATGAAGCAGGCTGACACCACACAGTACGTGCCAATGCTGGAAAG
  GAAGGAGGTTTCCAAATACTCCGACATCCAGAGATCGCTGTACGATCGGCCGGCCTCCTATAAGAAGAAA
  TCCATGCTAGACTCAGAAGCTAAAAACCTCCTTTCGGATGATGACTCCGAGGGTCTGACTTTGCTGGACC
  TACTAAGCTTCACCTACCAAGTGGCCCGAGGGATGGAATTTTTGGCTTCGAAAAATTGTGTCCACCGGGA
  CCTGGCTGCCCGCAACGTCCTTCTGGCGCAGGGGAAAATCGTGAAGATCTGTGACTTCGGGCTGGCCAGA
  GACATCATGCACGATTCTAACTACGTGTCAAAGGGCAGCACTTTCCTGCCTGTGAAGTGGATGGCACCTG
  AGAGCATTTTTGACAACCTCTACACCACGCTGAGTGACGTCTGGTCTTATGGCGTTCTGCTCTGGGAAAT
  ATTCTCCCTTGGTGGCACACCCTACCCTGGCATGATGGTTGATTCTACTTTCTACAATAAGATCAAGAGC
  GGATACAGGATGGCCAAACCCGACCATGCCACCAGTGAAGTCTATGAGATTATGGTGCAGTGCTGGAACA
  GTGAACCCGAAAAGAGACCCTCCTTCTACCACCTCAGCGAGATAGTGGAGAATCTGCTGCCTGGACAGTA
  CAAAAAGAGTTATGAAAAGATTCACCTGGACTTCCTAAAGAGTGACCATCCGGCTGTGGCACGCATGCGT
  GTGGACTCTGACAACGCGTACATCGGTGTCACTTACAAAAATGAAGAGGACAAGCTGAAGGAATGGGAAG
  GTGGCCTGGATGAACAGAGATTGAGCGCAGATAGTGGCTACATCATTCCCCTGCCAGACATTGACCCTGT
  TCCAGAGGAGGAAGACCTGGGCAAGAGGAACAGACACAGCTCGCAGACTTCGGAAGAGAGCGCCATCGAG
  ACGGGTTCCAGTAGTTCCACTTTCATCAAGAGAGAGGACGAGACCATTGAGGACATCGACATGATGGATG
  ATATTGGCATAGATTCCTCAGACCTGGTGGAGGACAGCTTCCTGTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_012802
• Insert Size: 3267 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012802.1, NP_036934.1
• RefSeq Size: 6532 bp
• RefSeq ORF: 3267 bp
• Locus ID: 25267
• UniProt ID: P20786
• Cytogenetics: 14p11
• Gene Summary: acts as a receptor tyrosine kinase for PDGF; may play a role in glial cell generation [RGD, Feb 2006]