Gapdh (NM_017008) Rat Untagged Clone

CAT#: RN206447

Gapdh (untagged ORF) - Rat glyceraldehyde-3-phosphate dehydrogenase (Gapdh), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gapdh
• Synonyms: BARS-38; Gapd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206447 representing NM_017008
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAAGGTCGGTGTGAACGGATTTGGCCGTATCGGACGCCTGGTTACCAGGGCTGCCTTCTCTTGTG
  ACAAAGTGGACATTGTTGCCATCAACGACCCCTTCATTGACCTCAACTACATGGTCTACATGTTCCAGTA
  TGACTCTACCCACGGCAAGTTCAACGGCACAGTCAAGGCTGAGAATGGGAAGCTGGTCATCAACGGGAAA
  CCCATCACCATCTTCCAGGAGCGAGATCCCGCTAACATCAAATGGGGTGATGCTGGTGCTGAGTATGTCG
  TGGAGTCTACTGGCGTCTTCACCACCATGGAGAAGGCTGGGGCTCACCTGAAGGGTGGGGCCAAAAGGGT
  CATCATCTCCGCCCCTTCCGCTGATGCCCCCATGTTTGTGATGGGTGTGAACCACGAGAAATATGACAAC
  TCCCTCAAGATTGTCAGCAATGCATCCTGCACCACCAACTGCTTAGCCCCCCTGGCCAAGGTCATCCATG
  ACAACTTTGGCATCGTGGAAGGGCTCATGACCACAGTCCATGCCATCACTGCCACTCAGAAGACTGTGGA
  TGGCCCCTCTGGAAAGCTGTGGCGTGATGGCCGTGGGGCAGCCCAGAACATCATCCCTGCATCCACTGGT
  GCTGCCAAGGCTGTGGGCAAGGTCATCCCAGAGCTGAACGGGAAGCTCACTGGCATGGCCTTCCGTGTTC
  CTACCCCCAATGTATCCGTTGTGGATCTGACATGCCGCCTGGAGAAACCTGCCAAGTATGATGACATCAA
  GAAGGTGGTGAAGCAGGCGGCCGAGGGCCCACTAAAGGGCATCCTGGGCTACACTGAGGACCAGGTTGTC
  TCCTGTGACTTCAACAGCAACTCCCATTCTTCCACCTTTGATGCTGGGGCTGGCATTGCTCTCAATGACA
  ACTTTGTGAAGCTCATTTCCTGGTATGACAATGAATATGGCTACAGCAACAGGGTGGTGGACCTCATGGC
  CTACATGGCCTCCAAGGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_017008
• Insert Size: 1002 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017008.3, NP_058704.1
• RefSeq Size: 1306 bp
• RefSeq ORF: 1002 bp
• Locus ID: 24383
• UniProt ID: P04797
• Cytogenetics: 4q42
• Gene Summary: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies in human and mouse have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins. Many pseudogenes similar to this locus are found throughout the rat genome. [provided by RefSeq, Jan 2014]