Hspd1 (NM_022229) Rat Untagged Clone

CAT#: RN206138

Hspd1 (untagged ORF) - Rat heat shock protein 1 (chaperonin) (Hspd1), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Hspd1
• Synonyms: Hsp60; Hspd1-30p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206138 representing NM_022229
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCGACTACCCACAGTCCTTCGCCAGATGAGACCAGTGTCTCGGGCACTGGCTCCTCATCTCACTC
  GGGCCTATGCCAAAGATGTAAAATTTGGTGCGGATGCTCGAGCCTTAATGCTTCAAGGTGTAGACCTTTT
  AGCCGATGCTGTAGCTGTTACAATGGGGCCAAAGGGAAGAACAGTGATTATTGAACAGAGTTGGGGAAGT
  CCCAAAGTAACAAAAGATGGGGTCACTGTTGCAAAGTCAATTGATTTAAAGGATAAATACAAAAATATCG
  GAGCTAAGCTTGTTCAGGATGTTGCCAATAACACAAATGAAGAGGCTGGGGATGGCACCACCACTGCCAC
  TGTTCTGGCACGGTCTATTGCCAAGGAGGGCTTTGAGAAGATCAGCAAAGGGGCTAATCCAGTGGAAATC
  CGGAGAGGTGTGATGTTGGCTGTTGATGCTGTAATTGCTGAACTTAAGAAACAATCTAAACCTGTGACAA
  CCCCTGAAGAAATTGCTCAGGTTGCTACAATTTCTGCAAACGGAGACAAAGACATTGGGAACATCATTTC
  TGATGCAATGAAGAAGGTTGGAAGAAAGGGTGTCATCACAGTGAAGGATGGAAAAACCCTGAATGATGAG
  CTAGAAATTATTGAAGGCATGAAGTTTGATAGAGGATATATTTCCCCATATTTTATTAACACATCAAAAG
  GTCAAAAATGTGAATTCCAAGATGCCTATGTTTTGTTGAGTGAAAAGAAAATTTCTAGTGTTCAGTCCAT
  TGTACCTGCTCTTGAAATTGCCAATGCTCACCGGAAGCCCTTGGTCATAATTGCTGAAGATGTTGATGGA
  GAAGCTCTTAGCACACTGGTTTTGAACAGGCTAAAAGTTGGTCTTCAGGTTGTAGCAGTCAAAGCTCCAG
  GGTTTGGGGACAACAGGAAGAACCAGCTTAAAGATATGGCTATCGCTACTGGTGGTGCGGTGTTTGGAGA
  AGAGGGTTTGAATCTAAATCTTGAAGATGTTCAAGCTCATGATTTAGGGAAAGTTGGAGAGGTCATCGTC
  ACCAAAGATGATGCCATGCTTTTGAAAGGAAAAGGTGACAAAGCTCACATTGAAAAACGTATTCAAGAAA
  TCACTGAGCAGCTAGACATCACAACTAGTGAATATGAGAAGGAAAAGCTGAACGAGCGACTTGCTAAACT
  CTCAGATGGAGTAGCTGTGTTGAAGGTTGGAGGGACAAGTGATGTTGAAGTGAATGAGAAGAAAGACAGA
  GTTACAGATGCTCTCAATGCTACAAGAGCAGCTGTTGAAGAAGGCATTGTTCTAGGAGGGGGCTGTGCTC
  TACTTCGGTGCATCCCAGCCTTGGATTCATTAAAGCCTGCTAATGAAGATCAGAAGATAGGTATAGAAAT
  TATTAAAAGAGCACTTAAAATTCCTGCAATGACAATTGCTAAGAATGCAGGTGTTGAAGGATCTTTGATA
  GTTGAAAAAATTCTGCAGAGTTCCTCAGAGGTTGGCTATGATGCCATGCTTGGAGATTTTGTGAACATGG
  TGGAAAAGGGAATCATTGATCCAACAAAGGTTGTAAGAACTGCTTTACTGGATGCTGCTGGGGTGGCCTC
  CCTGCTAACTACAGCCGAAGCTGTAGTGACAGAAATTCCTAAAGAAGAGAAGGACCCTGGAATGGGTGCA
  ATGGGTGGAATGGGAGGGGGTATGGGAGGTGGCATGTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022229
• Insert Size: 1722 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022229.2, NP_071565.2
• RefSeq Size: 2255 bp
• RefSeq ORF: 1722 bp
• Locus ID: 63868
• UniProt ID: P63039
• Cytogenetics: 9q31
• Gene Summary: a stress-inducible molecular chaperone which participates in protein folding [RGD, Feb 2006]