Hspd1 (NM_022229) Rat Tagged ORF Clone

CAT#: RR206138

Hspd1 (Myc-DDK-tagged ORF) - Rat heat shock protein 1 (chaperonin) (Hspd1), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR206138

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hspd1
• Synonyms: Hsp60; Hspd1-30p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206138 representing NM_022229
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCGACTACCCACAGTCCTTCGCCAGATGAGACCAGTGTCTCGGGCACTGGCTCCTCATCTCACTC
  GGGCCTATGCCAAAGATGTAAAATTTGGTGCGGATGCTCGAGCCTTAATGCTTCAAGGTGTAGACCTTTT
  AGCCGATGCTGTAGCTGTTACAATGGGGCCAAAGGGAAGAACAGTGATTATTGAACAGAGTTGGGGAAGT
  CCCAAAGTAACAAAAGATGGGGTCACTGTTGCAAAGTCAATTGATTTAAAGGATAAATACAAAAATATCG
  GAGCTAAGCTTGTTCAGGATGTTGCCAATAACACAAATGAAGAGGCTGGGGATGGCACCACCACTGCCAC
  TGTTCTGGCACGGTCTATTGCCAAGGAGGGCTTTGAGAAGATCAGCAAAGGGGCTAATCCAGTGGAAATC
  CGGAGAGGTGTGATGTTGGCTGTTGATGCTGTAATTGCTGAACTTAAGAAACAATCTAAACCTGTGACAA
  CCCCTGAAGAAATTGCTCAGGTTGCTACAATTTCTGCAAACGGAGACAAAGACATTGGGAACATCATTTC
  TGATGCAATGAAGAAGGTTGGAAGAAAGGGTGTCATCACAGTGAAGGATGGAAAAACCCTGAATGATGAG
  CTAGAAATTATTGAAGGCATGAAGTTTGATAGAGGATATATTTCCCCATATTTTATTAACACATCAAAAG
  GTCAAAAATGTGAATTCCAAGATGCCTATGTTTTGTTGAGTGAAAAGAAAATTTCTAGTGTTCAGTCCAT
  TGTACCTGCTCTTGAAATTGCCAATGCTCACCGGAAGCCCTTGGTCATAATTGCTGAAGATGTTGATGGA
  GAAGCTCTTAGCACACTGGTTTTGAACAGGCTAAAAGTTGGTCTTCAGGTTGTAGCAGTCAAAGCTCCAG
  GGTTTGGGGACAACAGGAAGAACCAGCTTAAAGATATGGCTATCGCTACTGGTGGTGCGGTGTTTGGAGA
  AGAGGGTTTGAATCTAAATCTTGAAGATGTTCAAGCTCATGATTTAGGGAAAGTTGGAGAGGTCATCGTC
  ACCAAAGATGATGCCATGCTTTTGAAAGGAAAAGGTGACAAAGCTCACATTGAAAAACGTATTCAAGAAA
  TCACTGAGCAGCTAGACATCACAACTAGTGAATATGAGAAGGAAAAGCTGAACGAGCGACTTGCTAAACT
  CTCAGATGGAGTAGCTGTGTTGAAGGTTGGAGGGACAAGTGATGTTGAAGTGAATGAGAAGAAAGACAGA
  GTTACAGATGCTCTCAATGCTACAAGAGCAGCTGTTGAAGAAGGCATTGTTCTAGGAGGGGGCTGTGCTC
  TACTTCGGTGCATCCCAGCCTTGGATTCATTAAAGCCTGCTAATGAAGATCAGAAGATAGGTATAGAAAT
  TATTAAAAGAGCACTTAAAATTCCTGCAATGACAATTGCTAAGAATGCAGGTGTTGAAGGATCTTTGATA
  GTTGAAAAAATTCTGCAGAGTTCCTCAGAGGTTGGCTATGATGCCATGCTTGGAGATTTTGTGAACATGG
  TGGAAAAGGGAATCATTGATCCAACAAAGGTTGTAAGAACTGCTTTACTGGATGCTGCTGGGGTGGCCTC
  CCTGCTAACTACAGCCGAAGCTGTAGTGACAGAAATTCCTAAAGAAGAGAAGGACCCTGGAATGGGTGCA
  ATGGGTGGAATGGGAGGGGGTATGGGAGGTGGCATGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206138 representing NM_022229
  Red=Cloning site Green=Tags(s)
  
  MLRLPTVLRQMRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGS
  PKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEI
  RRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKDIGNIISDAMKKVGRKGVITVKDGKTLNDE
  LEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDG
  EALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLNLNLEDVQAHDLGKVGEVIV
  TKDDAMLLKGKGDKAHIEKRIQEITEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDR
  VTDALNATRAAVEEGIVLGGGCALLRCIPALDSLKPANEDQKIGIEIIKRALKIPAMTIAKNAGVEGSLI
  VEKILQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEAVVTEIPKEEKDPGMGA
  MGGMGGGMGGGMF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022229
• ORF Size: 1719 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022229.2, NP_071565.2
• RefSeq Size: 2255 bp
• RefSeq ORF: 1722 bp
• Locus ID: 63868
• UniProt ID: P63039
• Cytogenetics: 9q31
• MW: 61 kDa
• Gene Summary: a stress-inducible molecular chaperone which participates in protein folding [RGD, Feb 2006]