Alox5 (NM_012822) Rat Untagged Clone

CAT#: RN204994

Alox5 (untagged ORF) - Rat arachidonate 5-lipoxygenase (Alox5), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Alox5
• Synonyms: LOX5A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN204994 representing NM_012822
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTCCTACACTGTCACCGTAGCCACCGGTAGCCAGTGGTTCGCGGGCACCGACGACTACATTTACC
  TCAGCCTCATTGGCTCTGCAGGCTGCAGTGAGAAGCATCTTCTTGACAAAGCTTTCTACAATGACTTCGA
  GCGCGGCGGTCGCGACTCCTATGACGTCACTGTGGATGAAGAACTGGGTGAGATCTACCTAGTCAAAATC
  GAGAAGCGCAAATACAGGCTCCATGATGACTGGTACTTGAAATACATCACACTGAAGACACCCCACGACT
  ACATAGAGTTCCCTTGTTATCGTTGGATCACAGGCGAGGGCGAGATTGTCCTGAGGGATGGATGTGCAAA
  ATTGGCCCGAGATGACCAAATCCACATCCTCAAGCAGCACAGGCGGAAAGAACTGGAAACACGTCAGAAA
  CAGTATCGATGGATGGAGTGGAACCCCGGCTTCCCTTTGAGTATTGATGCTAAATGCCACAAGGATCTGC
  CCCGAGATATCCAGTTTGATAGTGAAAAGGGAGTGGACTTTGTTCTGAACTACTCAAAAGCGATGGAGAA
  CCTGTTCATCAATCGCTTCATGCACATGTTCCAGTCTTCCTGGCATGACTTTGCTGACTTTGAGAAAATC
  TTCGTCAAAATCAGCAACACTATTTCTGAGAGAGTCAAGAACCACTGGCAAGAGGACCTCATGTTTGGCT
  ACCAGTTCCTGAATGGCTGCAACCCAGTACTCATCAAGCGCTGCACAGAGTTGCCTAAGAAGCTCCCAGT
  GACCACAGAAATGGTGGAGTGCAGCCTAGAGCGGCAGCTCAGTTTAGAACAGGAAGTACAGGAAGGGAAC
  ATTTTCATCGTTGATTACGAACTACTGGATGGCATTGATGCTAACAAAACTGACCCCTGTACACACCAGT
  TCCTGGCCGCCCCCATCTGCCTGCTGTATAAGAACCTAGCCAACAAGATTGTTCCCATCGCCATCCAGCT
  CAACCAAACCCCTGGAGAGAAGAACCCAATTTTCCTCCCTACGGACTCAAAATACGACTGGCTTTTGGCC
  AAAATCTGGGTGCGTTCAAGTGACTTCCATATCCATCAAACAATCACCCATCTTCTCCGCACACATCTGG
  TGTCTGAGGTGTTCGGTATTGCCATGTACCGCCAGCTGCCTGCTGTGCACCCCCTTTTCAAGCTGCTGGT
  AGCCCATGTGAGGTTCACCATTGCCATCAACACTAAGGCCCGGGAACAGCTTAACTGTGAGTACGGCCTT
  TTTGACAAGGCCAATGCCACCGGAGGTGGTGGGCACGTGCAGATGGTGCAGAGAGCTGTCCAGGACCTGA
  CCTATTCCTCCCTGTGCTTCCCGGAGGCCATCAAGGCCCGGGGCATGGACAACACCGAGGACATCCCCTA
  CTACTTCTATCGTGATGATGGACTGCTCGTGTGGGAAGCTATCCAGTCGTTCACAACTGAGGTGGTAAGC
  ATCTACTATGAGGATGACCAGGTGGTGGAGGAGGACCAGGAACTGCAGGACTTCGTGAAGGATGTTTACG
  TTTATGGCATGCGGGGCAGAAAGGCCTCAGGTTTCCCCAAGTCCATCAAGAGCAGGGAGAAATTGTCTGA
  GTACCTGACGGTGGTGATCTTCACAGCCTCGGCCCAGCATGCAGCTGTAAACTTTGGCCAGTATGACTGG
  TGCTCCTGGATCCCCAACGCTCCTCCAACTATGCGGGCCCCACCACCCACGGCCAAGGGTGTGGTCACCA
  TCGAGCAGATTGTGGATACTCTACCAGACCGTGGCCGCTCATGTTGGCATCTAGGTGCAGTGTGGGCCTT
  GAGCCAGTTTCAAGAGAATGAGCTGTTTCTGGGCATGTACCCAGAGGAGCATTTCATCGAGAAGCCAGTG
  AAAGAAGCCATGATTCGATTCCGCAAGAACCTGGAGGCCATCGTCAGCGTGATTGCCGAGCGCAATAAGA
  ACAAAAAGCTCCCCTACTACTACCTGTCACCAGACAGATTCCAAACAGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012822
• Insert Size: 2013 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012822.1, NP_036954.1
• RefSeq Size: 2535 bp
• RefSeq ORF: 2013 bp
• Locus ID: 25290
• UniProt ID: P12527
• Cytogenetics: 4q42
• Gene Summary: catalyzes the conversion of arachidonate to leukotriene A4 in leukotriene metabolism [RGD, Feb 2006]