Trpa1 (NM_207608) Rat Untagged Clone

CAT#: RN203182

Trpa1 (untagged ORF) - Rat transient receptor potential cation channel, subfamily A, member 1 (Trpa1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Trpa1
• Synonyms: Anktm1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN203182 representing NM_207608
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGCAGCTTGAGGAGGGTTCTGCGCCCCGAGGAAAGAAAGGAGGTCCAGGGCGTCGTCTATCGCG
  GCGTGGGGAAAGACATGGACTGCTCCAAGGAATCCTTTAAGGTGGACATTGAAGGAGATATGTGCAGATT
  AGAAGCCTTCATCAAGAACCGAAGAAAACTAAGCAAGTACGAGGATGAAAATCTCTGTCTTCTGCATCAC
  GCCGCAGCCGAAGGTCAAGTTGAACTGATGCAATTGATCATCAATGGCTCTTCCTGTGAAGCGCTGAATG
  TAATGGATGATTATGGAAATACCCCACTACATTGGGCTGCAGAAAAAAATCAAGTTGAAAGTGTGAAGTT
  TCTTCTCAGCCAAGGAGCAAATCCAAACCTCCGAAATAGAAACATGATGGCACCCCTTCACATAGCTGTA
  CAGGGCATGTACAACGAAGTGATCAAGGTCTTGACCGAGCACAAGGCCACTAACATCAATTTAGAAGGAG
  AGAATGGGAACACAGCTTTGATGTCCACGTGTGCCAAAGACAACAGTGAAGCTTTGCAAATTTTGTTAGA
  AAAAGGAGCTAAGCTGTGTAAATCAAATAAATGGGGAGACTACCCTGTGCACCAGGCAGCATTTTCAGGT
  GCCAAAAGATGCATGGAATTAATCTTAGCATATGGTGAAAAGACCGGCTATAGCCGGGAGGCTCACATTA
  ACTTTGTGAATCATAAGAAAGCCAGCCCTCTCCACCTCGCAGTTCAGAGCGGCGACTTGGACATGATTAA
  GATGTGCCTGGACAGCGGTGCACACATCGACATGATGGAGAATGCCAAATGCATGGCCCTCCATTTTGCT
  GCAACCCAGGGAGCCACTGACATCGTTAAACTCATGATCTCATCCTATACTGGAAGCAGCGATATCGTGA
  ATGCAGTCGATGGCAATCAGGAGACCCTGCTTCACAGAGCCTCATTATTTGATCATCATGACCTGGCAGA
  CTACCTAATTTCAGTGGGAGCAGACATCAACAGCACTGATTCTGAAGGACGCTCTCCACTTATTTTAGCA
  ACTGCTTCTGCATCCTGGAATATTGTGAATTTGCTCCTCTCTAAAGGTGCCAAAGTAGACATAAAAGATC
  ATCTTGGGCGTAACTTTTTACATTTGACTGTGCAGCAGCCTTATGGGCTAAGGAATTTGCGGCCTGAGTT
  TTTGCAGATGCAACACATCAAGGAGCTGGTGATGGATGAGGACAATGATGGATGTACGCCTCTCCATTAT
  GCTTGTAGGCAGGGGGCCCCTGTCTCTGTAAATAACCTCCTCAGGTTCAATGTGTCCGTTCATTCCAAAA
  GCAAAGATAAGAAGTCTCCCCTGCACTTTGCCGCCAGCTATGGGCGCATCAATACATGTCAGAGGCTTCT
  GCAAGACATCAGCGACACAAGGCTTTTGAATGAAGGGGATCTCCATGGAATGACCCCTCTCCATCTGGCA
  GCAAAGAATGGACATGATAAAGTCGTTCAACTTCTTCTCAAGAAAGGGGCCTTGTTTCTTAGTGACCACA
  ATGGCTGGACTGCTTTGCATCATGCGTCCATGGGTGGCTACACTCAGACCATGAAGGTCATTCTTGACAC
  TAACTTGAAGTGCACAGACCGGCTAGATGAAGAAGGGAACACAGCACTTCACTTTGCAGCACGGGAAGGC
  CATGCAAAGGCTGTTGCGATGCTTTTGAGCTACAATGCTGACATCCTCCTGAACAAGAAGCAAGCTTCCT
  TTCTGCATATTGCACTGCACAATAAGCGCAAGGAAGTGGTTCTCACAACCATCAGGAGTAAAAGATGGGA
  TGAGTGTCTTCAAGTTTTTACTCATGATTCTCCAAGCAATCGCTGTCCAATCATGGAAATGGTGGAATAC
  CTCCCCGAGTGCATGAAAGTTCTTCTAGATTTCTGCATGATACCTTCCACAGAAGACAAGTCCTGCCAAG
  ACTACCATATTGAGTATAATTTCAAGTATCTCCAATGCCCATTATCCATGACCAAAAAAGTAACCCCCAC
  CCAGGATGTGATCTATGAGCCTCTTACAATCCTCAATGTCATGGTCCAACATAACCGCATAGAGCTCCTC
  AACCACCCTGTGTGTAGGGAATACTTACTTATGAAATGGTGTGCCTATGGCTTCAGAGCTCATATGATGA
  ACCTAGGATCTTATTGTCTTGGTCTCATACCCATGACCCTTCTTGTTGTCAAAATACAGCCTGGAATGGC
  CTTCAATTCTACTGGAATAATCAATGAAACTATTAGTACTCATGAGGAAAGAATAAACACTCTGAATTCG
  TTTCCATTAAAAATATGTATGATTCTAGTTTTTTTATCAAGTATATTTGGATATTGCAAAGAAGTGGTCC
  AAATTTTCCAACAGAAAAGGAACTACTTTCTGGACTACAACAATGCTCTGGAGTGGGTCATCTACACCAC
  CAGTATGATCTTCGTGTTGCCCTTATTCCTCGACATCCCCGCGTATATGCAGTGGCAATGTGGAGCGATA
  GCAATATTCTTCTACTGGATGAACTTCCTACTATATCTTCAAAGGTTTGAGAACTGTGGCATTTTCATTG
  TTATGTTGGAGGTGATTTTTAAAACATTGCTGAGATCGACGGGAGTGTTTATCTTCCTGCTATTGGCTTT
  TGGCCTCAGCTTTTACGTCCTCCTGAATTTCCAAGATGCCTTCAGCACCCCGTTGCTTTCCTTAATCCAG
  ACGTTCAGTATGATGCTGGGAGACATCAATTACCGAGATGCTTTCCTAGAACCGTTGTTCAGGAATGAGT
  TGGCATACCCGGTCCTCACCTTTGGGCAGCTTATTGCCTTCACAATGTTCGTCCCAATTGTTCTCATGAA
  CCTACTGATTGGTTTGGCAGTTGGGGACATTGCTGAGGTCCAGAAGCATGCATCATTAAAGAGGATTGCT
  ATGCAGGTGGAACTGCATACCAACTTAGAAAAAAAGCTACCATTCTGGTACTTGCGCAAAGTGGATCAGA
  GGTCCACCATCGTGTATCCGAATAGACCCAGGCACGGCAGGATGCTGCGGTTTTTTCATTACTTTCTTAG
  TATGCAAGAAACACGACAAGAAGCACCAAACATTGACACATGTTTGGAAATGGAAATACTGAAACAGAAA
  TACCGGCTGAAGGACCTCACTTCTCTTTTGGAAAAGCAGCACGAGCTCATCAAACTCATCATCCAAAAGA
  TGGAGATCATCTCGGAGACAGAAGATGAAGATAACCATTGCTCTTTCCAAGACAGGTTCAAAAAGGAACG
  GCTAGAACAAATGCATAGCAAATGGAATTTTGTCTTAAACGCAGTTAAGACTAAAACACATTGTTCTATT
  AGCCACCCAGACATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_207608
• Insert Size: 3378 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207608.1, NP_997491.1
• RefSeq Size: 3378 bp
• RefSeq ORF: 3378 bp
• Locus ID: 312896
• UniProt ID: Q6RI86
• Cytogenetics: 5q11
• Gene Summary: Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, cinnamaldehyde, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as an ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana (PubMed:14712238). Is also activated by menthol (in vitro) (By similarity). May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system (By similarity).[UniProtKB/Swiss-Prot Function]