Pold3 (NM_001024750) Rat Untagged Clone

CAT#: RN202890

Pold3 (untagged ORF) - Rat polymerase (DNA-directed), delta 3, accessory subunit (Pold3), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pold3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202890 representing NM_001024750
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAACAGCTGTATCTAGAAAACATAGACGAGTTCGTCACGGACCAAAACAAGATCGTGACTTACA
  AATGGCTAAGCTATACACTGGGAGTTCATGTTAACCAGGCCAAACAGATGCTCTATGAATATGTTGAAAG
  GAAACGGAAGGAAAATTCGGGAGCTCAGCTGCATGTTACCTACTTGGTGTCTGGCAGTCTTATACAGAAT
  GGACATTCTTGCCACAAGGTTGCAGTAGTGAGAGAAGATAGACTGGAAGCAGTGAAGTCCAAGTTAGCTG
  TGACTGCCAGCATCCATGTGTACAGCATCCAGAAAGCTATGCTAAAGGACAGTGGGCCTCTGTTCAATAC
  CGACTATGACATCCTTAAAAGCAATTTGCAGAACTGCAGCAAGTTTAGTGCCATACAGTGTGCAGCTGCT
  GTCCCCAGAGCTCCTGCAGAATCCTCATCCTCCAGAAAGTTTGAGCAATCAAATCTTCAGGCAGCAAGTG
  AGACACAAGCCAGTGAGCTGACTACCAATGGCCATGGTCCACCTGCCTCCAAACAGGCTTCCCAGCAGCC
  CAAAGGAATTATGGGAATGTTAATCTCTAAAGCTGCTACTAAAACCCAAGACACCAACAAGGACACCAAA
  ACAGAAGCCCGAGAAGTAACAAGTGCATCTTCCGCTGGGGGCAAAGCACCAGGAAAAGGGAATGTGATGA
  GCAACTTTTTTGGAAAAGCCGCCATGAGTAAACTTAAAGTCAATTTGGATTCAGAACAAGCAGTAAAGGA
  AGAAAAAACAGTGGAGCAACCTCCAGTGTCTGTCGCTGAACCAAAGCTGGCAGCTCCCCCAGCTCTGAAG
  AAATCCAGCAGAAAGTCAGAGCCTGTGAAGATGCAGCAGAAGGAAAAAAAGTGCAGGGGCAAGCGAGTAG
  ACTTATCTGATGAGGAAGCAAAGGAAACCGAAAACCTGAAGAAAAAGAGGAGAAGGATCAAGCTTCCTCA
  GTCCGATAGCAGTGAAGATGAAGTCTTCGCAGACTCTCCTGAGATGTTTGAAGCAGTCTCACCATCTCCA
  CCTCCTGCATCTCCACCTCCTGATCCTGTGCCAAAAACTGAGCCCCCTCCTGTCAAGCGCTCAAGTGGAG
  AAAACAAAAGAAAACGAAAGCGTGTACTGAAATCTAAAACCTTTGTGGATGAAGAAGGCTGCATGGTGAC
  TGAGAAAGTCTATGAGAGTGAGTCCTGCACAGACAGTGAGGAGGAGACGAAGATGAAGATGGCCTCAGCA
  CAAAGACCCCCTGCTGCCACTGTGAAACGGGAGCCCAGAGAAGAACGGAAGGGTCCCAAGAAAGGGGCTG
  CTGCTCTGGGCAAAGCCAACAGACAAGTTTCCATTACTGGCTTCTTCCAGAAAAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001024750
• Insert Size: 1389 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024750.1, NP_001019921.1
• RefSeq Size: 1743 bp
• RefSeq ORF: 1389 bp
• Locus ID: 293144
• Cytogenetics: 1q32