Mpi (NM_001004081) Rat Untagged Clone

CAT#: RN201799

Mpi (untagged ORF) - Rat mannose phosphate isomerase (mapped) (Mpi), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Mpi
• Synonyms: MGC94106
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN201799 representing NM_001004081
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAATCCGCGAGTGTTCCCACTTTCCTGTGTGGTGCAGCAGTATGCCTGGGGGAAGGTGGGCTCCA
  AAAGTGAAGTGGCATGCCTACTGGCTTGCAGTGACCCACTGACCCAGATCTCAGAGGACAAGCCTTATGC
  GGAGCTGTGGATGGGGGCACACCCCCGGGGAGATGCCAAGATCCTTGACAACCGTATTTCCCAAAAGACC
  TTAGGCCAGTGGATTGCTGAGAACCAGAACAGCTTAGGCCAAAAGGTCAAAGACACCTTTAACGGAAAGC
  TGCCCTTCCTCTTCAAAGTGCTCTCAGTGGAAACGGCCCTGTCTATCCAGGCACACCCTAACAAGGAGCT
  GGCAGAGAAGCTGCACCTTCAGGCTCCAGAGCACTACCCCGATGCCAACCACAAGCCAGAAATGGCCATT
  GCCCTTACACCCTTCCAAGGCTTGTGTGGTTTCCGGCCAGTGGAGGAGATTGTGACCTTTCTGAAGAAGG
  TGCCTGAGTTCCAGTCACTGATTGGAGAGGATGCCACAGCACAGCTGAAGAAGAGCATGAATGAGGGTTC
  TGGGGCCATGGCCTCTGCTCTGAAGAACTGCTTTTCCCACCTGATGAAGAGTGAGAAGAAGGTGGTGGTG
  GAGCAGCTTAACCTGTTGGTGAAACGGATCTCCCAGCAAATATCTAACGGAAACAGCATGGATGACATTT
  GTGGGGAGCTCTTGCTACAGCTGCACCAGCAGTACCCGGGTGATATCGGATGTTTCGCCATCTACCTCCT
  GAACCTGATCACCTTGAAGCCTGGGGAAGCCATGTTTCTGGAGGCCAACGTGCCCCATGCCTACCTGAAG
  GGAGACTGCGTGGAATGCATGGCATGTTCAGACAATACCGTGCGTGCTGGCCTGACACCCAAATTCATTG
  ATGTGTCAACCCTGTGTGAAATGCTCGACTACACACCTAGCCCCAGCAAAGACAGACTGTTTGCACCAAC
  ACTGAGTCAAGATGACCCCTACCTCTCTATCTACGATCCCCCTGTGCCAGACTTCACAGTTATGAAGATA
  GAGGTCCCTGGCTCCGTCACTGAATACAAAGTCTTGACACTAGACTCTGCCAGCATCCTTCTGTTGGTCC
  AAGGGACAGTGACAGCTATCATACCTTCAGTCCAAGGAGAAATCCCTCTGTCCCGTGGTGGAGTGCTCTT
  CATTGGGGCCAATGAGACTGTCTTACTGAAGCTTACCGTACCAAAGAATCTGCTGATATTCCGGGCCTGC
  TGTCTGCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001004081
• Insert Size: 1272 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004081.1, NP_001004081.1
• RefSeq Size: 1420 bp
• RefSeq ORF: 1272 bp
• Locus ID: 300741
• UniProt ID: Q68FX1
• Cytogenetics: 8q24
• Gene Summary: Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.[UniProtKB/Swiss-Prot Function]