Tnk2 (NM_001008336) Rat Untagged Clone

CAT#: RN201546

Tnk2 (untagged ORF) - Rat tyrosine kinase, non-receptor, 2 (Tnk2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Tnk2
• Synonyms: MGC94214
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN201546 representing NM_001008336
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCGGAGGAGGGAACAGGCTGGCTGCTGGAGCTGCTGTCTGAGGTGCAGCTACAACAGTATTTCC
  TGAGGCTCCGAGATGACCTCAACATTACCCGCCTGTCTCATTTTGAGTATGTCAAAAACGAAGACCTGGA
  AAAGATTGGCATGGGCCGGCCTGGCCAGAGGCGGCTATGGGAGGCTGTGAAGAGGAGGAAGGCCATGTGC
  AAACGCAAGTCATGGATGAGCAAGGTGTTCAGTGGAAAGCGGCAGGAGGCTGAGTTCCCTTCCCATCACT
  CTCAGAGCACCTTCCGGAAGCCCTCCCCCACCCCAGGGGGCCTGGCAGGGGAGGGGACCCTGCAGAGCCT
  CACCTGCCTCATTGGGGAGAAAGACCTGCGCCTGCTGGAGAAGCTGGGAGATGGCTCCTTTGGCGTGGTG
  CGCAGGGGTGAATGGGATGCCCCTGCAGGGAAGACGGTGAGTGTGGCCGTGAAGTGCCTGAAACCTGATG
  TGCTGAGCCAGCCCGAGGCCATGGACGACTTCATCCGGGAGGTCAATGCCATGCATTCGCTAGACCACCG
  AAACCTCATTCGCTTGTACGGTGTGGTGCTCACACCACCCATGAAGATGGTGACAGAGCTGGCCCCTCTG
  GGATCCTTGTTGGACCGGCTACGTAAACACCAAGGTCATTTCCTCTTGGGGACGCTGAGTCGCTATGCCG
  TGCAGGTGGCTGAGGGCATGGGCTACCTGGAGTCCAAGCGCTTCATTCACCGAGACCTGGCTGCTCGAAA
  TCTGCTTTTGGCCACCCGTGACCTGGTCAAGATTGGGGACTTTGGACTGATGCGAGCTCTGCCCCAGAAT
  GATGGCCACTATGTCATGCAAGAACATCGCAAGGTGCCCTTTGCCTGGTGTGCCCCTGAGAGCCTGAAGA
  CACGGACTTTCTCCCATGCCAGTGACACCTGGATGTTTGGGGTCACACTGTGGGAGATGTTTACGTATGG
  CCAGGAGCCCTGGATTGGCCTCAATGGCAGCCAGATCCTGCATAAGATTGACAAGGAAGGGGAGCGCCTG
  CCCCGGCCTGAGGACTGCCCCCAAGACATCTACAATGTCATGGTCCAGTGCTGGGCCCACAAGCCAGAGG
  ACAGACCCACATTTGTGGCCCTTCGGGACTTCCTGCTGGAGGCTCAGCCTACAGACATGCGGGCCCTTCA
  GGACTTCGAGGAGCCGGACAAGCTGCACATCCAGATGAACGACGTCATTACTGTCATTGAGGGAAGGGCT
  GAGAACTACTGGTGGCGTGGTCAGAACACACGGACGCTGTGTGTGGGACCCTTCCCTCGAAATGTGGTGA
  CCTCTGTGGCTGGCCTGTCAGCCCAGGACATCAGCCAGCCTCTACAGAATAGCTTCATTCACACAGGGCA
  TGGCGACAGTGACCCCCGCCACTGCTGGGGGTTCCCTGACAGGATCGATGAACTGTACCTGGGAAACCCC
  ATGGACCCCCCTGACCTGCTGAGTGTGGAACTGAGCACCTCGCGACCCACCCAGCATTTAGGACGGATGA
  AAAAGCCAACATATGACCCTGTGAGTGAGGACCCAGACCCCCTGTCCAGCGACTTCAAGAGACTTGGCCT
  GAGGAAGCCAGCCCTGCCCCGAGGGCTCTGGCTGGCAAAGCCCTCAGCCCGGGTGCCAGGCACCAAGGCA
  GGCCGCAGCAGTGGGGGTGAGGTCACACTCATCGACTTCGGCGAGGAGCCTGTAGCCCCAACCCCACGGC
  CCTGTGCACCTTCCTTGGCACAGTTGGCCATGGATGCCTGCTCCCTGCTGGACAAGACACCACCACAGAG
  CCCCACACGGGCACTGCCACGACCTTTACATCCTACACCTGTGGTGGACTGGGACGCTCGCCCGCTGCCC
  CCGCCCCCTGCCTACGATGATGTGGCCCAAGATGAGGATGACTTCGAGGTGTGCTCTATCAACAGCACCC
  TAGTGAGTGCAGGCCTCCCTACCGGGCCTAGCCAAGGCGAGACCAATTATGCGTTTGTACCTGAGCAGGC
  CCAGCTGCCCCCTGCCCTGGAGGATAACCTGTTCCTGCCACCGCAGGGTGGAGGCCAGCCACCCAGCTCA
  GCCCAGACTGCAGAGATTTTCCAGGCACTGCAGCAGGAGTGTATGCGGCAGCTACAGGTCCCCACTGGCC
  AGCTGACCCCGTCCCCTACCCCAGGAGGTGATGACAAGCCCCAGGTGCCACCCCGGGTACCTATTCCCCC
  TCGGCCCACACGTCCACGTGTGGGACTATCTCCAGCTCCCTTAGGTGAGGAAGAGGCAAGTCGGTGGCCT
  GGACCCTCCTCCCCTCCCCGAGTGCCTCCCCGGGAACCCCTGTCTCCTCAAGGGTCGAGGACCCCAAGCC
  CCCTAGTACCACCTGGCAGCTCCCCACTACCGCATCGGCTCTCGAGCTCACCTGGAAAGACCATGCCCAC
  CACCCAGAGCTTTGCCTCAGACCCTAAGTACGCCACTCCACAAGTGATCCAGGCTCCTGGCCCACGGGCT
  GGCCCCTGCATCCTGCCCATTGTCCGTGATGGCAGGAAGGTCAGCAGCACCCACTATTACCTGCTGCCAG
  AGCGCCCTCCCTACCTCGAGCGCTACCAGCGCTTCCTGAGGGAGGCCCAGAGCCCCGAGGAGCCGGCCGC
  CCTGCCCGTTCCCCCACTGCTGCCCCCGCCTAGCACTCCAGCCCCTGCCGCCCCCACTGCCACCGTCCGA
  CCTATGCCTCAGGCTGCCCCAGACCCAAAGGCCAACTTCTCCACCAATAACAGCAACCCAGGGGCGCAGC
  CACCATCCCTGAGGGCCAGTGCACGGCTGCCGCAGAGGGGCTGCCCAGGGGATGGGCAAGAGGCTGCCCG
  GCCAGCAGACAAAGTCCAGATGCTGCAGGCCATGGTGCATGGGGTGACCACAGAGGAGTGCCAGGCGGCC
  CTGAGGAGCCACAGCTGGAGTATTCAGAGGGCTGCCCAGTATCTGAAGGTGGAGCAGCTCTTTGGGCTGG
  GTCTTCGGCCACGGGTGGAGTGCCACAAAGTGCTAGAGATGTTCGACTGGAACCTAGAGCAAGCCGGCTG
  TCACCTTCTGGGGTCCTGTGGCCCTGCCCACCACAAACGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001008336
• Insert Size: 3123 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008336.1, NP_001008337.1
• RefSeq Size: 4250 bp
• RefSeq ORF: 3123 bp
• Locus ID: 303882
• UniProt ID: Q5U2X5
• Cytogenetics: 11q22
• Gene Summary: Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363', thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR (By similarity).[UniProtKB/Swiss-Prot Function]