Gsk3a (NM_017344) Rat Untagged Clone

CAT#: RN201401

Gsk3a (untagged ORF) - Rat glycogen synthase kinase 3 alpha (Gsk3a), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gsk3a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN201401 representing NM_017344
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGCGGCGGGCCTTCGGGAGGTGGCCCTGGGGGCTCGGGCCGGGCGCGGACCAGCTCGTTCGCGG
  AGCCAGGCGGCGGAGGCGGAGGCGGTGGCGGCGGCCCCGGGGGCTCGGCCTCCGGCCCAGGAGGCACTGG
  CGGCGGGAAGGCGTCAGTCGGGGCTATGGGTGGGGGCGTGGGAGCCTCGAGCTCTGGGGGTGGCCCCAGC
  GGCAGCGGCGGAGGAGGCAGCGGTGGCCCCGGCGCGGGCACTAGCTTCCCGCCGCCCGGAGTGAAGCTGG
  GCCGTGACAGCGGGAAGGTGACCACAGTGGTAGCCACTCTAGGCCAAGGCCCAGAGCGTTCCCAAGAGGT
  GGCTTACACCGACATCAAAGTGATTGGCAATGGCTCATTCGGAGTAGTGTACCAGGCACGGCTGGCAGAA
  ACGAGGGAACTGGTGGCCATCAAGAAGGTTCTTCAGGACAAAAGGTTCAAGAACCGAGAGCTGCAGATTA
  TGCGTAAGCTGGACCACTGCAATATCGTGAGGCTGCGGTACTTTTTCTACTCCAGTGGGGAGAAGAAAGA
  TGAGCTGTATTTAAATCTGGTGCTGGAATATGTGCCCGAGACCGTGTACCGAGTGGCCCGTCACTTTACC
  AAGGCCAAGTTGATCATCCCTATCATCTATGTCAAGGTGTACATGTACCAGCTCTTCCGGAGCTTGGCCT
  ACATCCACTCCCAAGGTGTGTGTCACCGTGACATCAAGCCCCAGAATTTGCTTGTGGACCCTGACACTGC
  TGTCCTCAAGCTCTGCGACTTTGGCAGTGCAAAGCAGTTGGTTCGGGGGGAGCCCAACGTGTCCTACATC
  TGTTCTCGGTACTACCGTGCTCCGGAGCTCATCTTTGGAGCCACAGATTACACCTCGTCCATCGATGTGT
  GGTCAGCTGGCTGTGTACTGGCTGAGCTGCTTCTTGGCCAGCCCATCTTCCCTGGGGACAGTGGGGTAGA
  CCAGCTTGTGGAGATCATCAAGGTACTAGGGACGCCAACCAGGGAGCAAATCCGAGAGATGAACCCTAAC
  TACACAGAATTCAAGTTCCCCCAGATCAAAGCTCACCCCTGGACAAAGGTGTTCAAATCTCGGACACCAC
  CTGAGGCCATCGCACTCTGCTCTAGCCTGCTGGAGTACACTCCATCCTCAAGGCTCTCCCCACTAGAGGC
  CTGTGCCCACAGTTTCTTTGATGAACTGCGGAGTCTCGGAACCCAGCTCCCCAACAACCGCCCGCTTCCC
  CCCCTCTTCAACTTCAGTCCTGGTGAACTTTCCATCCAACCGTCTCTCAATGCCATTCTCATCCCTCCTC
  ACTTGAGGTCCCCATCAGGCCCTGCTACCCTCACCTCGTCCTCACAAGCTTTAACTGAGACTCAGACTGG
  CCAAGACTGGCAGGCACCTGATGCCACACCTACCCTCACTAACTCTTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_017344
• Insert Size: 1452 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017344.1, NP_059040.1
• RefSeq Size: 2155 bp
• RefSeq ORF: 1452 bp
• Locus ID: 50686
• UniProt ID: P18265
• Cytogenetics: 1q21
• Gene Summary: protein-serine kinase; may mediate hormonal control of several regulatory proteins including glycogen synthase and the transcription factor c-jun [RGD, Feb 2006]