Smad3 (NM_013095) Rat Untagged Clone

CAT#: RN201118

Smad3 (untagged ORF) - Rat SMAD family member 3 (Smad3), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Smad3
• Synonyms: mad3; Madh3; Smad 3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN201118 representing NM_013095
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCCATCCTGCCCTTCACCCCCCCGATCGTGAAGCGCCTGCTGGGCTGGAAGAAGGGCGAGCAGA
  ACGGGCAGGAGGAGAAGTGGTGCGAGAAGGCGGTCAAGAGCTTGGTGAAGAAGCTCAAGAAGACGGGGCA
  GTTGGACGAGCTGGAGAAGGCCATCACCACGCAGAACGTGAACACCAAGTGCATTACCATCCCCAGGTCA
  CTGGATGGCCGGCTGCAGGTATCCCATCGGAAGGGGCTCCCCCATGTCATCTACTGCCGCTTGTGGCGGT
  GGCCCGACCTGCACAGCCACCATGAGTTACGGGCCATGGAGCTCTGTGAGTTCGCCTTCAACATGAAGAA
  GGATGAAGTGTGTGTAAATCCTTACCACTATCAGAGAGTAGAGACACCAGTGCTACCTCCAGTGTTGGTG
  CCACGCCACACCGAGATCCCGGCCGAGTTCCCCCCTCTGGATGACTACAGCCATTCCATCCCCGAGAACA
  CTAACTTCCCCGCTGGCATTGAACCCCAGAGCAATATTCCAGAAACCCCACCTCCTGGCTACCTGAGTGA
  AGATGGAGAAACTAGCGACCACCAGATGAACCACAGCATGGACGCAGGCTCTCCAAACCTCTCCCCGAAT
  CCGATGTCCCCAGCACACAATAACTTGGACCTACAGCCAGTCACCTACTGTGAGCCGGCCTTTTGGTGCT
  CCATCTCCTACTACGAGCTGAACCAGCGAGTTGGGGAGACATTCCACGCTTCACAGCCGTCCATGACAGT
  AGATGGCTTCACTGATCCCTCCAATTCAGAGCGCTTCTGTCTGGGCCTGCTGTCCAATGTTAACCGGAAT
  GCAGCCGTGGAGCTTACAAGGCGGCACATTGGGAGGGGTGTGCGGCTCTACTACATTGGAGGGGAGGTCT
  TTGCAGAGTGCCTCAGTGACAGTGCTATTTTCGTCCAGTCTCCCAACTGCAACCAGCGCTATGGCTGGCA
  CCCTGCCACTGTCTGCAAGATACCCCCAGGCTGCAACCTGAAGATCTTCAACAACCAGGAATTTGCTGCC
  CTCCTAGCTCAGTCTGTCAACCAGGGCTTTGAGGCTGTCTACCAGTTGACTCGCATGTGCACCATCCGCA
  TGAGCTTCGTCAAAGGCTGGGGAGCCGAGTACAGGAGACAGACAGTGACCAGCACCCCCTGCTGGATCGA
  GCTACACCTGAATGGGCCCTTGCAGTGGCTTGACAAGGTCCTCACCCAGATGGGTTCCCCCAGCATCCGC
  TGTTCCAGCGTGTCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013095
• Insert Size: 1278 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013095.3, NP_037227.1
• RefSeq Size: 5037 bp
• RefSeq ORF: 1278 bp
• Locus ID: 25631
• UniProt ID: P84025
• Cytogenetics: 8q24
• Gene Summary: transcription factor; important component of the TGF-beta signaling pathway [RGD, Feb 2006]