Acly (NM_016987) Rat Untagged Clone

CAT#: RN200531

Acly (untagged ORF) - Rat ATP citrate lyase (Acly), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Acly
• Synonyms: ACL; Clatp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200531 representing NM_016987
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTTTACAAGTACATCTGTACCACCTCAG
  CCATCCAGAACCGGTTCAAGTATGCCCGGGTTACTCCCGACACAGACTGGGCCCATCTCCTGCAGGACCA
  CCCCTGGCTGCTTAGCCAGAGCTTGGTAGTCAAGCCGGACCAGCTGATCAAACGTCGAGGAAAGCTTGGT
  CTAGTCGGGGTCAACCTCTCTCTGGATGGAGTCAAATCCTGGCTGAAACCTCGACTGGGACATGAGGCCA
  CCGTCGGCAAGGCCAAAGGCTTCCTCAAGAACTTTCTGATTGAGCCCTTCGTCCCCCACAGTCAGGCGGA
  GGAGTTCTACGTGTGCATCTATGCTACCCGGGAAGGAGACTACGTCCTGTTCCACCATGAAGGGGGTGTG
  GATGTGGGCGATGTGGACACCAAAGCCCAGAAGCTGCTTGTGGGTGTGGACGAGAAACTGAACGCTGAAG
  ACATTAAGAGACACCTGTTGGTCCACGCCCCCGAAGACAAGAAAGAAATCCTGGCCAGCTTCATCTCCGG
  CCTATTCAATTTCTACGAAGATCTTTACTTCACCTACCTTGAGATCAACCCCCTTGTGGTGACCAAAGAT
  GGTGTCTACATCCTTGACCTGGCGGCCAAGGTGGACGCCACTGCTGACTACATCTGCAAAGTCAAGTGGG
  GTGATATAGAGTTCCCTCCCCCCTTTGGGCGTGAGGCATACCCAGAGGAAGCCTACATTGCAGACCTGGA
  TGCCAAAAGTGGGGCGAGCTTGAAGCTGACCTTGCTGAACCCCAAGGGGCGGATCTGGACCATGGTTGCC
  GGGGGTGGCGCCTCTGTCGTGTACAGTGATACCATCTGTGATCTTGGAGGTGTCAACGAACTGGCGAATT
  ACGGGGAGTACTCTGGTGCCCCCAGTGAACAACAGACCTATGACTACGCCAAGACCATCCTCTCACTTAT
  GACTCGAGAGAAGCACCCGGATGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAACGTG
  GCCGCCACCTTCAAGGGCATTGTGAGAGCAATTCGAGATTACCAGGGTCCCCTGAAGGAGCACGAGGTCA
  CCATCTTTGTTCGAAGAGGTGGCCCGAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGGAAGAC
  CACTGGAATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGC
  CACCGGCCCATTCCCAACCAGCCACCCACAGCGGCTCACACTGCCAACTTCCTCCTTAATGCCAGTGGGA
  GCACATCGACACCAGCACCCAGCAGGACAGCGTCTTTTTCCGAGTCCAGAGCTGACGAGGTGGCCCCTGC
  AAAGAAAGCCAAGCCAGCCATGCCCCAAGATTCAGTCCCAAGTCCAAGATCCCTGCAAGGAAAGAGTGCC
  ACCCTCTTCAGCCGACATACCAAGGCTATCGTGTGGGGCATGCAGACCCGGGCTGTGCAAGGCATGCTGG
  ACTTTGACTACGTGTGCTCCCGAGATGAGCCTTCAGTGGCTGCTATGGTCTACCCGTTCACGGGGGATCA
  TAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATG
  AAAAAGCATCCGGAGGTAGACGTGCTGATCAACTTTGCATCTCTGCGATCGGCTTATGACAGCACCATGG
  AGACCATGAACTATGCACAGATCCGGACCATAGCCATCATAGCAGAAGGCATCCCTGAGGCTCTCACACG
  GAAGCTCATCAAGAAGGCAGACCAGAAGGGCGTGACCATCATTGGGCCAGCCACGGTTGGGGGCATCAAG
  CCTGGATGCTTTAAGATTGGGAATACTGGTGGGATGCTGGACAACATCCTGGCCTCCAAACTGTATCGCC
  CAGGCAGTGTGGCCTACGTCTCGCGTTCAGGAGGCATGTCTAACGAACTCAATAATATCATCTCTCGGAC
  CACAGATGGTGTCTACGAGGGTGTTGCCATCGGCGGGGACAGGTACCCTGGGTCCACATTCATGGATCAC
  GTGCTGCGTTACCAAGACACTCCAGGAGTCAAGATGATTGTAGTTCTTGGGGAGATAGGGGGTACAGAAG
  AATATAAGATCTGCCGGGGCATCAAGGAGGGCCGCCTCACCAAGCCAGTGGTCTGCTGGTGCATCGGGAC
  CTGTGCCACCATGTTCTCTTCTGAGGTCCAGTTTGGCCACGCTGGGGCTTGTGCCAACCAGGCTTCTGAA
  ACGGCAGTAGCCAAGAACCAGGCCTTGAAGGAAGCGGGAGTGTTTGTGCCCCGAAGCTTTGATGAGCTCG
  GAGAAATCATTCAGTCCGTGTATGAAGATCTTGTGGCCAAAGGCGCCATTGTACCTGCTCAGGAAGTGCC
  ACCTCCAACAGTACCCATGGACTACTCTTGGGCCAGGGAGCTGGGTTTAATCCGAAAACCTGCCTCATTC
  ATGACCAGCATCTGTGACGAGCGGGGGCAGGAACTCATTTATGCGGGCATGCCCATCACCGAGGTCTTCA
  AGGAAGAGATGGGCATTGGTGGTGTCCTGGGCCTCCTCTGGTTCCAGAGAAGGTTGCCCAAGTATTCCTG
  CCAGTTCATTGAGATGTGTCTCATGGTCACCGCTGATCACGGGCCAGCTGTCTCCGGGGCCCATAACACT
  ATCATCTGTGCTCGGGCTGGGAAGGACCTGGTCTCCAGCCTCACCTCAGGGCTGCTCACCATTGGGGACC
  GGTTTGGGGGTGCCTTGGACGCAGCAGCGAAGATGTTCAGTAAAGCCTTTGACAGCGGCATTATTCCCAT
  GGAGTTTGTGAACAAGATGAAGAAGGAGGGGAAACTGATCATGGGCATCGGCCATCGAGTCAAATCGATA
  AACAACCCAGACATGCGAGTGCAGATCCTCAAAGACTTTGTCAAACAGCACTTCCCCGCCACCCCGCTGC
  TCGACTATGCACTGGAAGTGGAGAAAATCACCACCTCAAAGAAGCCAAATCTTATCCTGAACGTGGATGG
  TTTCATCGGCGTTGCGTTTGTGGACATGCTTAGGAACTGTGGCTCCTTCACCCGGGAGGAAGCTGACGAG
  TATGTTGACATTGGAGCCCTCAATGGCGTCTTTGTGCTGGGAAGGAGTATGGGCTTCATCGGGCACTATC
  TTGACCAGAAGAGGCTGAAGCAAGGGCTGTATCGTCACCCCTGGGACGACATTTCCTATGTTCTCCCGGA
  ACACATGAGCATGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016987
• Insert Size: 3306 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016987.2, NP_058683.2
• RefSeq Size: 4331 bp
• RefSeq ORF: 3306 bp
• Locus ID: 24159
• UniProt ID: P16638
• Cytogenetics: 10q31
• Gene Summary: catalyzes the synthesis of cytosolic acetyl-CoA; plays a role in lipid and cholesterol metabolism [RGD, Feb 2006]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).