Acly (NM_016987) Rat Tagged ORF Clone

CAT#: RR200531

Acly (Myc-DDK-tagged ORF) - Rat ATP citrate lyase (Acly), transcript variant 1, (10 ug)

Product Images

Circular map for RR200531

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acly
• Synonyms: ACL; Clatp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200531 representing NM_016987
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTTTACAAGTACATCTGTACCACCTCAG
  CCATCCAGAACCGGTTCAAGTATGCCCGGGTTACTCCCGACACAGACTGGGCCCATCTCCTGCAGGACCA
  CCCCTGGCTGCTTAGCCAGAGCTTGGTAGTCAAGCCGGACCAGCTGATCAAACGTCGAGGAAAGCTTGGT
  CTAGTCGGGGTCAACCTCTCTCTGGATGGAGTCAAATCCTGGCTGAAACCTCGACTGGGACATGAGGCCA
  CCGTCGGCAAGGCCAAAGGCTTCCTCAAGAACTTTCTGATTGAGCCCTTCGTCCCCCACAGTCAGGCGGA
  GGAGTTCTACGTGTGCATCTATGCTACCCGGGAAGGAGACTACGTCCTGTTCCACCATGAAGGGGGTGTG
  GATGTGGGCGATGTGGACACCAAAGCCCAGAAGCTGCTTGTGGGTGTGGACGAGAAACTGAACGCTGAAG
  ACATTAAGAGACACCTGTTGGTCCACGCCCCCGAAGACAAGAAAGAAATCCTGGCCAGCTTCATCTCCGG
  CCTATTCAATTTCTACGAAGATCTTTACTTCACCTACCTTGAGATCAACCCCCTTGTGGTGACCAAAGAT
  GGTGTCTACATCCTTGACCTGGCGGCCAAGGTGGACGCCACTGCTGACTACATCTGCAAAGTCAAGTGGG
  GTGATATAGAGTTCCCTCCCCCCTTTGGGCGTGAGGCATACCCAGAGGAAGCCTACATTGCAGACCTGGA
  TGCCAAAAGTGGGGCGAGCTTGAAGCTGACCTTGCTGAACCCCAAGGGGCGGATCTGGACCATGGTTGCC
  GGGGGTGGCGCCTCTGTCGTGTACAGTGATACCATCTGTGATCTTGGAGGTGTCAACGAACTGGCGAATT
  ACGGGGAGTACTCTGGTGCCCCCAGTGAACAACAGACCTATGACTACGCCAAGACCATCCTCTCACTTAT
  GACTCGAGAGAAGCACCCGGATGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAACGTG
  GCCGCCACCTTCAAGGGCATTGTGAGAGCAATTCGAGATTACCAGGGTCCCCTGAAGGAGCACGAGGTCA
  CCATCTTTGTTCGAAGAGGTGGCCCGAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGGAAGAC
  CACTGGAATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGC
  CACCGGCCCATTCCCAACCAGCCACCCACAGCGGCTCACACTGCCAACTTCCTCCTTAATGCCAGTGGGA
  GCACATCGACACCAGCACCCAGCAGGACAGCGTCTTTTTCCGAGTCCAGAGCTGACGAGGTGGCCCCTGC
  AAAGAAAGCCAAGCCAGCCATGCCCCAAGATTCAGTCCCAAGTCCAAGATCCCTGCAAGGAAAGAGTGCC
  ACCCTCTTCAGCCGACATACCAAGGCTATCGTGTGGGGCATGCAGACCCGGGCTGTGCAAGGCATGCTGG
  ACTTTGACTACGTGTGCTCCCGAGATGAGCCTTCAGTGGCTGCTATGGTCTACCCGTTCACGGGGGATCA
  TAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATG
  AAAAAGCATCCGGAGGTAGACGTGCTGATCAACTTTGCATCTCTGCGATCGGCTTATGACAGCACCATGG
  AGACCATGAACTATGCACAGATCCGGACCATAGCCATCATAGCAGAAGGCATCCCTGAGGCTCTCACACG
  GAAGCTCATCAAGAAGGCAGACCAGAAGGGCGTGACCATCATTGGGCCAGCCACGGTTGGGGGCATCAAG
  CCTGGATGCTTTAAGATTGGGAATACTGGTGGGATGCTGGACAACATCCTGGCCTCCAAACTGTATCGCC
  CAGGCAGTGTGGCCTACGTCTCGCGTTCAGGAGGCATGTCTAACGAACTCAATAATATCATCTCTCGGAC
  CACAGATGGTGTCTACGAGGGTGTTGCCATCGGCGGGGACAGGTACCCTGGGTCCACATTCATGGATCAC
  GTGCTGCGTTACCAAGACACTCCAGGAGTCAAGATGATTGTAGTTCTTGGGGAGATAGGGGGTACAGAAG
  AATATAAGATCTGCCGGGGCATCAAGGAGGGCCGCCTCACCAAGCCAGTGGTCTGCTGGTGCATCGGGAC
  CTGTGCCACCATGTTCTCTTCTGAGGTCCAGTTTGGCCACGCTGGGGCTTGTGCCAACCAGGCTTCTGAA
  ACGGCAGTAGCCAAGAACCAGGCCTTGAAGGAAGCGGGAGTGTTTGTGCCCCGAAGCTTTGATGAGCTCG
  GAGAAATCATTCAGTCCGTGTATGAAGATCTTGTGGCCAAAGGCGCCATTGTACCTGCTCAGGAAGTGCC
  ACCTCCAACAGTACCCATGGACTACTCTTGGGCCAGGGAGCTGGGTTTAATCCGAAAACCTGCCTCATTC
  ATGACCAGCATCTGTGACGAGCGGGGGCAGGAACTCATTTATGCGGGCATGCCCATCACCGAGGTCTTCA
  AGGAAGAGATGGGCATTGGTGGTGTCCTGGGCCTCCTCTGGTTCCAGAGAAGGTTGCCCAAGTATTCCTG
  CCAGTTCATTGAGATGTGTCTCATGGTCACCGCTGATCACGGGCCAGCTGTCTCCGGGGCCCATAACACT
  ATCATCTGTGCTCGGGCTGGGAAGGACCTGGTCTCCAGCCTCACCTCAGGGCTGCTCACCATTGGGGACC
  GGTTTGGGGGTGCCTTGGACGCAGCAGCGAAGATGTTCAGTAAAGCCTTTGACAGCGGCATTATTCCCAT
  GGAGTTTGTGAACAAGATGAAGAAGGAGGGGAAACTGATCATGGGCATCGGCCATCGAGTCAAATCGATA
  AACAACCCAGACATGCGAGTGCAGATCCTCAAAGACTTTGTCAAACAGCACTTCCCCGCCACCCCGCTGC
  TCGACTATGCACTGGAAGTGGAGAAAATCACCACCTCAAAGAAGCCAAATCTTATCCTGAACGTGGATGG
  TTTCATCGGCGTTGCGTTTGTGGACATGCTTAGGAACTGTGGCTCCTTCACCCGGGAGGAAGCTGACGAG
  TATGTTGACATTGGAGCCCTCAATGGCGTCTTTGTGCTGGGAAGGAGTATGGGCTTCATCGGGCACTATC
  TTGACCAGAAGAGGCTGAAGCAAGGGCTGTATCGTCACCCCTGGGACGACATTTCCTATGTTCTCCCGGA
  ACACATGAGCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200531 representing NM_016987
  Red=Cloning site Green=Tags(s)
  
  MSAKAISEQTGKELLYKYICTTSAIQNRFKYARVTPDTDWAHLLQDHPWLLSQSLVVKPDQLIKRRGKLG
  LVGVNLSLDGVKSWLKPRLGHEATVGKAKGFLKNFLIEPFVPHSQAEEFYVCIYATREGDYVLFHHEGGV
  DVGDVDTKAQKLLVGVDEKLNAEDIKRHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKD
  GVYILDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVA
  GGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNV
  AATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALG
  HRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRADEVAPAKKAKPAMPQDSVPSPRSLQGKSA
  TLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAM
  KKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIK
  PGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDH
  VLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPVVCWCIGTCATMFSSEVQFGHAGACANQASE
  TAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVAKGAIVPAQEVPPPTVPMDYSWARELGLIRKPASF
  MTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQRRLPKYSCQFIEMCLMVTADHGPAVSGAHNT
  IICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSI
  NNPDMRVQILKDFVKQHFPATPLLDYALEVEKITTSKKPNLILNVDGFIGVAFVDMLRNCGSFTREEADE
  YVDIGALNGVFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016987
• ORF Size: 3303 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016987.2, NP_058683.2
• RefSeq Size: 4331 bp
• RefSeq ORF: 3306 bp
• Locus ID: 24159
• UniProt ID: P16638
• Cytogenetics: 10q31
• MW: 120.8 kDa
• Gene Summary: catalyzes the synthesis of cytosolic acetyl-CoA; plays a role in lipid and cholesterol metabolism [RGD, Feb 2006]