Gnas (NM_021845) Rat Untagged Clone

CAT#: RN200466

Gnas (untagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gnas
• Synonyms: ALEX; G-alpha-8; Gnas1; Gnpas; Nesp55; SCG6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200466 representing NM_021845
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATGCTCAACTGCCTCCACGGCAATAATATGTCAGGACAACACGATATCCCCCCTGAAGTCGGGG
  ACCAGCCCGAGCAAGAACCTTTGGAAGCCCAAGGGGCCGCTGCCCCCGGTGCTGGGGTTGGCCCAGCCGA
  AGAAATGGAGACCGAACCGTCTAACAATGAGCCCATCCCCGACGAGACTGACAGTGAGGTCTGTGGACCC
  CCTGAAGACTCCAAATCTGACATCCAGAGCCCCAGTCAGGCCTTCGAGGAAGTCCAAGTGGGCGGAGACT
  ACAGCCCACCTCCAGAGGAAGCCATGCCATTCGAGATCCAACAGCCCAGCCTGGGAGATTTCTGGCCCAC
  CCTGGAGCAGCCTGGACCATCTGGGACCCCATCAGGCATCAAAGCCTTCAACCCAGCGATTTTGGAGCCC
  GGGACCCCCACTGGTGCACACCCAGGCCTGGGAGCCTATAGCCCCCCACCAGAAGAAGCTATGCCATTTG
  AGTTCAATGAGCCTGCCCAGGAAGACCGTTGCCAGCCTCCCTTGCAAGTCCCAGACCTTGCGCCAGGGGG
  TCCGGAAGCATGGGTCTCCAGAGCTCTTCCCGCGGAGCCCGGGAACCTCGGATTTGAAAACACTGGCTTC
  CGAGAAGACTACAGCCCTCCACCTGAAGAATCTGTGCCATTTCAGCTCGATGGAGAAGAATTCGGGGGCG
  ATAGCCCACCCCCAGGACTCCCGCGAGTCACGCCACAAATCGGCATTGGCGGCGAGTTCCCGACAGTCGC
  GGTCCCGAGTACGCTCTGCCTCGCTCCCGCCGCGAACGCGCCTCCCCTCTGGGTCCAGGGCGCCATTGGC
  AGACCATTCCGCGAGGCTGTCAGATCTCCTAACTTCGCATACGACATTTCCCCAATGGAGATCACCAGAC
  CCCTGCTTGAGATTGGCAGAGCCTCCACTGGGGTCGACGACGACACCGCTGTCAATATGGACAGCCCCCC
  AATCGCAAGTGATGGCCCGCCCATCGAAGTCTCGGGAGCCCCAGTTAAGAGCGAGCACGCAAAGAGACCC
  CCACTTGAGCGACAAGCAGCCGAGACAGGAAACAGCCCTATCAGCAGCACCACTGCAGAGGAGGCAAAAG
  TCCCCTCCCTCGAGCGAGGAGAAGGATCCCCCACCCAACCTGAAACAGTGCATATCAAGCCAGCTCCTGT
  TGCGGAGTCCGGAACGGACTCTTCCAAAGCCGATCCGGACTCAGCTACACACGCAGTTCTTCAGATCGGT
  CCTGAGGAAGTCGGAGGAGTCCCAACCATGCCCACCGATCTTCCGCCTGCTTCTGAAGATGCGGGCCCAG
  ATGTCCGGGCAGAACCAGACGGAGGGACAGCCCCAGCCACCCCTGCCGAGTCCGAAGACAACAGAGAACC
  AGCCGCCGCCGCCGCCGCCGAGCCAGCCGCCGAGCCAGCCGCTGAGCCAGCCGCCGAGCCAGCCGCCGAG
  CCAGCCGCCGAGCCAGCTGCCGAGGCAGTCCCTGACACCGAAGCCGAGTCTGCCTCCGGGGCAGTCCCCG
  ACACCCAAGAGGAGCCCGCAGCCGCGGCAGCCTCTGCCACGCCGGCGGAGCCTGCCGCCCGGGCAGCCCC
  CGTCACCCCTACGGAGCCCGCTACCCGGGCTGTCCCTTCTGCCAGAGCCCATCCAGCCGCCGGGGCTGTC
  CCTGGAGCCTCAGCGATGTCAGCCGCTGCTAGGGCAGCCGCCGCTAGAGCAGCCTATGCAGGTCCTCTGG
  TCTGGGGAGCCAGGTCACTCTCGGCTACTCCAGCCGCTCGGGCATCCCTCCCTGCCCGCGCAGCAGCTGC
  CGCCCGAGCAGCCTCTGCTGCCCGCGCAGTCGCTGCCGGCCGGTCAGCCTCTGCCGCCCCAAGCAGGGCC
  CATCTTAGACCCCCCAGCCCGGAGATCCAGGTTGCTGACCCGCCTACTCCGCGGCCTGCTCCGCGGCCGA
  GTGCCTGGCCTGACAAATACGAACGTGGCCGAAGCTGCTGCAGGTATGAGGCTGCGTCCGGCATCTGCGA
  GATCGAGTCCTCCAGCGATGAGTCGGAAGAAGGGGCCACTGGCTGCTTCCAGTGGCTTCTGCGGCGAAAC
  CGCCGCCCTGGCCAGCCCCGGAGCCACACAGTCGGGAGCAACCCGGTCCGCAACTTCTTCGCCCGAGCCT
  TCGGAAGCTGCTTCGGTCTATCTGAGTGTACCCGATCACGATCCCTCAGCCCCGGGAAGGCCAAGGATCC
  TATGGAAGAGAGGCGCAAACAGATGCGCAAAGAAGCCATGGAGATGCGAGAGCAGAAGCGCGCAGATAAG
  AAACGCAGCAAGCTCATCGACAAGCAACTGGAGGAGGAGAAGATGGACTACATGTGTACACACCGCCTGC
  TGCTTCTAGGTGCTGGAGAGTCTGGCAAAAGCACCATTGTGAAGCAGATGAGGATCCTACATGTTAATGG
  GTTTAACGGAGAGGGCGGCGAAGAGGACCCGCAGGCTGCAAGGAGCAACAGCGATGGTGAGAAGGCCACC
  AAAGTGCAGGACATCAAAAACAACCTGAAGGAGGCCATTGAAACCATTGTGGCCGCCATGAGCAACCTGG
  TGCCCCCCGTGGAGCTGGCCAACCCTGAGAACCAGTTCAGAGTGGACTACATTCTGAGCGTGATGAACGT
  GCCAAACTTTGACTTCCCACCTGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAGGGAGTTCGT
  GCCTGCTACGAGCGCTCCAACGAGTACCAGCTGATCGACTGTGCCCAGTACTTCCTGGACAAGATTGATG
  TGATCAAGCAGGCCGACTACGTGCCAAGTGACCAGGACCTGCTTCGCTGCCGCGTCCTGACCTCTGGAAT
  CTTTGAGACCAAGTTCCAGGTGGACAAAGTCAACTTCCACATGTTCGATGTGGGCGGCCAGCGCGATGAA
  CGCCGCAAGTGGATCCAGTGCTTCAATGATGTGACTGCCATCATCTTCGTGGTGGCCAGCAGCAGCTACA
  ACATGGTCATCCGGGAGGACAACCAGACCAACCGTCTGCAGGAGGCTCTGAACCTCTTCAAGAGCATCTG
  GAACAACAGATGGCTGCGTACCATCTCTGTGATCCTCTTCCTCAACAAGCAAGATCTGCTTGCTGAGAAG
  GTCCTCGCTGGGAAATCGAAGATTGAGGACTACTTTCCAGAGTTCGCTCGCTACACCACTCCTGAGGATG
  CGACTCCCGAGCCCGGAGAGGACCCACGCGTGACCCGGGCCAAGTACTTCATCCGGGATGAGTTTCTGAG
  AATCAGCACTGCTAGTGGAGATGGACGTCACTACTGCTACCCTCACTTTACCTGCGCCGTGGACACTGAG
  AACATCCGCCGTGTCTTCAACGACTGCCGTGACATCATCCAGCGCATGCATCTTCGCCAATACGAGCTGC
  TCTAA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_021845
• Insert Size: 3435 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021845.5, NP_068617.6
• RefSeq Size: 3826 bp
• RefSeq ORF: 3435 bp
• Locus ID: 24896
• Cytogenetics: 3q43
• Gene Summary: This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene. [provided by RefSeq, Apr 2009]
  Transcript Variant: This variant (1, also known as GNASXL) is paternally expressed. It includes an alternate 5' exon, as compared to variant 3. This variant includes two overlapping open reading frames encoding XLas and Alex, respectively. This RefSeq represents XLas, also known as alpha s XXL, which is a neuroendocrine-specific G-protein alpha subunit; it has a distinct and much longer N-terminus, as compared to isoform GNASL. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.