Pparg (NM_013124) Rat Untagged Clone

CAT#: RN200398

Pparg (untagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pparg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200398 representing NM_013124
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGAAACTCTGGGAGATCCTCCTGTTGACCCAGAGCATGGTGCCTTCGCTGATGCACTGCCTATGA
  GCACTTCACAAGAAATTACCATGGTTGACACAGAGATGCCATTCTGGCCCACCAACTTCGGAATCAGCTC
  TGTGGACCTCTCTGTGATGGATGACCACTCCCATTCCTTTGACATCAAACCCTTTACCACGGTTGATTTC
  TCCAGCATTTCTGCTCCACACTATGAAGACATCCCGTTCACAAGAGCTGACCCAATGGTTGCTGATTACA
  AATATGACCTGAAGCTCCAAGAATACCAAAGTGCGATCAAAGTAGAGCCTGCGTCCCCGCCTTATTATTC
  TGAAAAAACCCAACTCTACAACAGGCCACATGAAGAGCCTTCAAACTCCCTCATGGCCATCGAGTGCCGA
  GTCTGTGGGGATAAAGCATCAGGCTTCCACTATGGAGTCCATGCTTGTGAAGGATGCAAGGGTTTTTTCC
  GAAGAACCATCCGATTGAAGCTTATTTATGATAGGTGTGATCTTAACTGTCGGATCCACAAAAAGAGTAG
  AAATAAATGTCAGTACTGTCGGTTTCAGAAGTGCCTTGCTGTGGGGATGTCTCACAATGCCATCAGGTTT
  GGGCGAATGCCACAGGCCGAGAAGGAGAAGCTGTTGGCGGAGATCTCCAGTGATATCGACCAGCTGAACC
  CAGAGTCTGCTGATCTGCGAGCCCTGGCAAAGCATTTGTATGACTCATACATAAAGTCCTTCCCGCTGAC
  CAAAGCCAAGGCGAGGGCGATCTTGACAGGAAAGACAACAGACAAATCACCATTTGTCATCTACGACATG
  AATTCCTTAATGATGGGAGAAGACAAAATCAAGTTCAAACATATCACCCCCCTGCAGGAGCAGAGCAAAG
  AGGTGGCCATCCGCATTTTTCAAGGGTGCCAGTTTCGATCCGTGGAAGCTGTGCAAGAGATCACAGAGTA
  TGCCAAAAATATCCCTGGTTTCATTAACCTTGACTTGAATGACCAAGTGACTCTGCTCAAGTATGGTGTC
  CATGAGATCATCTACACCATGCTGGCCTCCCTGATGAATAAAGATGGAGTCCTCATATCAGAGGGACAAG
  GATTCATGACCAGGGAGTTCCTCAAAAGCCTGCGGAAGCCCTTTGGTGACTTTATGGAGCCTAAGTTTGA
  GTTTGCTGTGAAGTTCAATGCACTGGAATTAGATGACAGTGACTTGGCCATATTTATAGCTGTCATTATT
  CTCAGTGGAGACCGCCCAGGCTTGCTGAACGTGAAGCCCATCGAGGACATCCAAGACAACCTGCTGCAGG
  CCCTGGAACTCCAGCTGAAGCTGAACCACCCGGAGTCCTCCCAGCTGTTCGCCAAGGTGCTCCAGAAGAT
  GACAGACCTCAGGCAGATTGTCACAGAGCACGTGCAGCTACTGCATGTGATCAAGAAGACGGAGACAGAT
  ATGAGCCTTCACCCTCTGCTCCAGGAGATCTACAAGGACTTGTATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013124
• Insert Size: 1518 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013124.3, NP_037256.1
• RefSeq Size: 1805 bp
• RefSeq ORF: 1518 bp
• Locus ID: 25664
• UniProt ID: O88275
• Cytogenetics: 4q42
• Gene Summary: ligand-activated transcription factor; mediates expression of genes involved in lipid metabolism [RGD, Feb 2006]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.