PIP5KI gamma (PIP5K1C) (NM_001300849) Human Tagged ORF Clone

CAT#: RG239373

PIP5K1C (tGFP-tagged) - Human phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (PIP5K1C), transcript variant 3

Product Images

Circular map for RG239373

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PIP5KI gamma
• Synonyms: LCCS3; PIP5K-GAMMA; PIP5K1-gamma; PIP5Kgamma
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239373 representing NM_001300849.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGCTGGAGGTACCGGACGAGGCGGAGAGCGCTGAGGCGGGGGCCGTGCCCTCGGAGGCGGCGTGG
  GCGGCAGAGAGCGGGGCGGCGGCAGGTTTGGCTCAGAAGAAGGCGGCCCCAACAGAGGTTCTGTCCATG
  ACGGCACAGCCGGGCCCTGGCCATGGGAAGAAGTTGGGCCATCGAGGTGTGGACGCATCCGGCGAAACC
  ACCTACAAGAAGACCACCTCCTCCACCCTGAAGGGTGCCATCCAGCTGGGCATCGGCTACACCGTGGGC
  CACCTGAGCTCCAAGCCCGAACGCGACGTGCTCATGCAGGACTTCTACGTGGTGGAGAGCATCTTCTTC
  CCCAGCGAAGGCAGCAACCTCACCCCCGCCCACCACTTCCAGGACTTCCGCTTCAAGACCTATGCACCT
  GTCGCCTTCCGCTACTTCCGGGAGCTCTTTGGGATCCGGCCAGATGATTACTTGTACTCCCTGTGCAAT
  GAGCCGCTGATCGAGCTGTCCAACCCGGGCGCCAGTGGCTCCCTCTTCTACGTCACCAGCGACGACGAG
  TTCATCATCAAGACCGTCATGCACAAGGAGGCCGAGTTCCTGCAGAAGCTGCTCCCTGGCTACTACATG
  AACCTCAACCAGAACCCGCGGACGCTGCTGCCCAAGTTCTATGGGCTGTACTGCGTGCAGTCGGGGGGC
  AAGAACATCCGCGTCGTGGTCATGAACAACATCCTGCCCCGCGTGGTCAAGATGCACCTCAAGTTCGAC
  CTCAAGGGCTCCACCTACAAGCGGCGCGCCAGCAAGAAGGAGAAGGAGAAGAGCTTCCCCACCTACAAG
  GACCTGGACTTCATGCAGGACATGCCCGAGGGGCTCCTGCTGGACGCCGACACCTTCAGCGCCCTGGTC
  AAGACGCTGCAGCGGGACTGCCTGGTCCTGGAAAGTTTCAAGATCATGGACTACAGCCTGCTGCTGGGC
  GTGCACAACATCGACCAGCACGAGCGCGAGCGGCAGGCGCAGGGCGCCCAGAGCACCTCAGATGAGAAG
  CGGCCTGTGGGCCAGAAGGCGCTCTACTCCACGGCCATGGAGTCCATCCAGGGTGGCGCCGCGCGCGGG
  GAGGCCATCGAATCGGATGACACGATGGGCGGGATCCCCGCTGTGAACGGCCGCGGGGAGCGGCTGCTG
  CTGCACATTGGCATCATCGACATCCTGCAGTCCTACAGGTTCATCAAGAAACTGGAGCACACCTGGAAG
  GCCCTCGTCCACGATGGGGACACGGTGTCCGTCCACCGCCCCAGCTTCTATGCCGAGCGCTTTTTCAAG
  TTCATGAGCAACACGGTCTTTCGGAAGAACTCCTCCCTGAAGTCCTCGCCCTCCAAGAAGGGGCGCGGC
  GGAGCCTTGCTAGCTGTGAAACCGCTGGGGCCCACCGCTGCCTTCTCGGCCAGCCAGATCCCTAGCGAG
  CGGGAGGAGGCCCAGTACGACCTGCGGGGGGCCCGCAGCTACCCCACGCTGGAGGACGAAGGCCGGCCC
  GACCTCCTGCCCTGCACGCCACCTTCTTTCGAAGAAGCCACTACAGCCTCCATTGCCACGACTCTGTCA
  TCCACATCCCTCTCCATTCCTGAGCGGTCCCCCTCGGAGACGTCGGAGCAGCCGCGGTACAGGCGGCGC
  ACACAGTCGTCTGGACAGGATGGCAGGCCGCAGGAGGAGCCACCCGCGGAAGAGGATCTGCAGCAGATT
  ACAGTGCAGGTGGAGCCTGCGTGCAGCGTGGAGATTGTGGTCCCCAAAGAGGAGGACGCAGGGGTGGAG
  GCTTCCCCGGCCGGTGCCTCTGCTGCTGTTGAAGTAGAAACTGCCAGCCAGGCCTCAGACGAGGAGGGC
  GCACCTGCCAGCCAGGCCTCGGACGAGGAGGACGCGCCCGCCACCGACATCTACTTTTTCACGGATGGG
  AGGTACTGGATTTACTCTCCCCGCCATCGCCGACTGCGGGCCGTGACGCTGAGCGCCTCGGGGACTGTA
  AGTGACCGCAGCCGGCCACCCTGGGGAGAAGGGGCAGTGCCCCTCGGGCAGCAGGGAGCCGCAGGTCCC
  CGGCCGGAAGCTCAGTGTCTGACGTCAGTTGTTTTCCAGAAGGGCTTTGGG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239373
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MELEVPDEAESAEAGAVPSEAAWAAESGAAAGLAQKKAAPTEVLSMTAQPGPGHGKKLGHRGVDASGET
  TYKKTTSSTLKGAIQLGIGYTVGHLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAP
  VAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSLFYVTSDDEFIIKTVMHKEAEFLQKLLPGYYM
  NLNQNPRTLLPKFYGLYCVQSGGKNIRVVVMNNILPRVVKMHLKFDLKGSTYKRRASKKEKEKSFPTYK
  DLDFMQDMPEGLLLDADTFSALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQHERERQAQGAQSTSDEK
  RPVGQKALYSTAMESIQGGAARGEAIESDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWK
  ALVHDGDTVSVHRPSFYAERFFKFMSNTVFRKNSSLKSSPSKKGRGGALLAVKPLGPTAAFSASQIPSE
  REEAQYDLRGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSETSEQPRYRRR
  TQSSGQDGRPQEEPPAEEDLQQITVQVEPACSVEIVVPKEEDAGVEASPAGASAAVEVETASQASDEEG
  APASQASDEEDAPATDIYFFTDGRYWIYSPRHRRLRAVTLSASGTVSDRSRPPWGEGAVPLGQQGAAGP
  RPEAQCLTSVVFQKGFG
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300849
• ORF Size: 2121 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001300849.2
• RefSeq Size: 3035 bp
• RefSeq ORF: 2124 bp
• Locus ID: 23396
• UniProt ID: O60331
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis, Focal adhesion, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton
• MW: 77.9 kDa
• Gene Summary: This locus encodes a type I phosphatidylinositol 4-phosphate 5-kinase. The encoded protein catalyzes phosphorylation of phosphatidylinositol 4-phosphate, producing phosphatidylinositol 4,5-bisphosphate. This enzyme is found at synapses and has been found to play roles in endocytosis and cell migration. Mutations at this locus have been associated with lethal congenital contractural syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Sep 2010]