GAK (NM_005255) Human Tagged ORF Clone

CAT#: RG223572

GAK (tGFP-tagged) - Human cyclin G associated kinase (GAK)

Product Images

Circular map for RG223572

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GAK
• Synonyms: DNAJ26; DNAJC26
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223572 representing NM_005255
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCTGCTGCAGTCGGCGCTCGACTTCTTGGCGGGTCCAGGCTCCCTGGGCGGTGCTTCCGGCCGCG
  ACCAGAGTGACTTCGTGGGGCAGACGGTGGAACTGGGCGAGCTGCGGCTGCGGGTGCGGCGGGTCCTGGC
  CGAAGGAGGGTTTGCATTTGTGTATGAAGCTCAAGATGTGGGGAGTGGCAGAGAGTATGCATTAAAGAGG
  CTATTATCCAATGAAGAGGAAAAGAACAGAGCCATCATTCAAGAAGTTTGCTTCATGAAAAAGCTTTCCG
  GCCACCCGAACATTGTCCAGTTTTGTTCTGCAGCGTCTATAGGAAAAGAGGAGTCAGACACGGGGCAGGC
  TGAGTTCCTCTTGCTCACAGAGCTCTGTAAAGGGCAGCTGGTGGAATTTTTGAAGAAAATGGAATCTCGA
  GGCCCCCTTTCGTGCGACACGGTTCTGAAGATCTTCTACCAGACGTGCCGCGCCGTGCAGCACATGCACC
  GGCAGAAGCCGCCCATCATCCACAGGGACCTCAAGGTTGAGAACTTGTTGCTTAGTAACCAAGGGACCAT
  TAAGCTGTGTGACTTTGGCAGTGCCACGACCATCTCGCACTACCCTGACTACAGCTGGAGCGCCCAGAGG
  CGAGCCCTGGTGGAGGAAGAGATCACGAGGAATACAACACCAATGTATAGAACACCAGAAATCATAGACT
  TGTATTCCAACTTCCCGATCGGCGAGAAGCAGGATATCTGGGCCCTGGGCTGCATCTTGTACCTGCTGTG
  CTTCCGGCAGCACCCTTTTGAGGATGGAGCGAAACTTCGAATAGTCAATGGGAAGTACTCGATCCCCCCG
  CACGACACGCAGTACACGGTCTTCCACAGCCTCATCCGCGCCATGCTGCAGGTGAACCCGGAGGAGCGGC
  TGTCCATCGCCGAGGTGGTGCACCAGCTGCAGGAGATCGCGGCCGCCCGCAACGTGAACCCCAAGTCTCC
  CATCACAGAGCTCCTGGAGCAGAATGGAGGCTACGGGAGCGCCACACTGTCCCGAGGGCCACCCCCTCCC
  GTGGGCCCCGCTGGCAGTGGCTACAGTGGAGGCCTGGCGCTGGCGGAGTACGACCAGCCGTATGGCGGCT
  TCCTGGACATTCTGCGGGGTGGGACAGAGCGGCTCTTCACCAACCTCAAGGACACCTCCTCCAAGGTCAT
  CCAGTCTGTCGCTAATTATGCAAAGGGTGACCTGGACATATCTTACATCACATCCAGAATTGCAGTGATG
  TCATTCCCAGCAGAAGGTGTGGAGTCAGCGCTCAAAAACAACATCGAAGATGTGCGGTTGTTCCTGGACT
  CCAAGCACCCAGGGCACTATGCCGTCTACAACCTGTCCCCGAGGACCTACCGGCCCTCCAGGTTCCACAA
  CCGGGTCTCCGAGTGTGGCTGGGCAGCACGGCGGGCCCCACACCTGCACACCCTGTACAACATCTGCAGG
  AACATGCACGCCTGGCTGCGGCAGGACCACAAGAACGTCTGCGTCGTGCACTGCATGGACGGGAGAGCCG
  CGTCTGCTGTGGCCGTCTGCTCCTTCCTGTGCTTCTGCCGTCTCTTCAGCACCGCGGAGGCCGCCGTGTA
  CATGTTCAGCATGAAGCGCTGCCCACCAGGCATCTGGCCATCCCACAAAAGGTACATCGAGTACATGTGT
  GACATGGTGGCGGAGGAGCCCATCACACCCCACAGCAAGCCCATCCTGGTGAGGGCCGTGGTCATGACAC
  CCGTGCCGCTGTTCAGCAAGCAGAGGAGCGGCTGCAGGCCCTTCTGCGAGGTCTACGTGGGGGACGAGCG
  TGTGGCCAGCACCTCCCAGGAGTACGACAAGATGCGGGACTTTAAGATTGAAGATGGCAAAGCGGTGATT
  CCCCTGGGCGTCACGGTGCAAGGAGACGTGCTCATCGTCATCTATCACGCCCGGTCCACTCTGGGCGGCC
  GGCTGCAGGCCAAGATGGCATCCATGAAGATGTTCCAGATTCAGTTCCACACGGGGTTTGTGCCTCGGAA
  CGCCACCACTGTGAAATTTGCCAAGTATGACCTGGACGCGTGTGACATTCAAGAAAAATACCCGGATTTA
  TTTCAAGTGAACCTGGAAGTGGAGGTGGAGCCCAGGGACAGGCCGAGCCGGGAAGCCCCACCATGGGAGA
  ACTCGAGCATGAGGGGGCTGAACCCCAAAATCCTGTTTTCCAGCCGGGAGGAGCAGCAAGACATTCTGTC
  TAAGTTTGGGAAGCCGGAGCTTCCCCGGCAGCCTGGCTCCACGGCTCAGTATGATGCTGGGGCAGGGTCC
  CCGGAAGCCGAACCCACAGACTCTGACTCACCGCCAAGCAGCAGCGCGGACGCCAGTCGCTTCCTGCACA
  CGCTGGACTGGCAGGAAGAGAAGGAGGCAGAGACTGGTGCAGAAAATGCCTCTTCCAAGGAGAGCGAGTC
  TGCCCTGATGGAGGACAGAGACGAGAGTGAGGTGTCAGATGAAGGGGGATCCCCGATCTCCAGCGAGGGC
  CAGGAACCCAGGGCCGACCCAGAGCCCCCCGGCCTGGCAGCAGGGCTGGTGCAGCAGGACTTGGTTTTTG
  AGGTGGAGACACCGGCTGTGCTGCCAGAGCCTGTGCCACAGGAAGACGGGGTCGACCTCCTGGGCCTGCA
  CTCCGAGGTGGGCGCAGGGCCAGCTGTACCCCCGCAGGCCTGCAAGGCCCCCTCCAGCAACACCGACCTG
  CTCAGCTGCCTCCTTGGGCCCCCTGAGGCCGCCTCCCAGGGGCCCCCGGAGGATCTGCTCAGCGAGGACC
  CGCTGCTCCTGGCAAGCCCGGCCCCTCCCCTGAGCGTGCAGAGCACCCCAAGAGGAGGGCCCCCTGCCGC
  TGGCAACAACTCCCAGCCCTGCTCCAATCCTGATCTCTTCGGCGAATTTCTCAATTCGGACTCTGTGACC
  GTCCCACCATCCTTCCCGTCTGCCCACAGCGCTCCGCCCCCATCCTGCAGCGCCGACTTCCTGCACCTGG
  GGGATCTGCCAGGAGAGCCCAGCAAGATGACAGCCTCGTCCAGCAACCCAGACCTGCTGGGAGGATGGGC
  TGCCTGGACCGAGACTGCAGCGTCGGCAGTGGCCCCCACGCCAGCCACAGAAGGCCCCCTCTTCTCTCCT
  GGAGGTCAGCCGGCCCCTTGTGGCTCTCAGGCCAGCTGGACCAAGTCTCAGAACCCGGACCCATTTGCTG
  ACCTTGGCGACCTCAGCTCCGGCCTCCAAGGCTCACCAGCTGGATTCCCTCCTGGGGGCTTCATTCCCAA
  AACGGCCACCACGCCCAAAGGCAGCAGCTCCTGGCAGACAAGTCGGCCGCCAGCCCAGGGCGCCTCATGG
  CCCCCTCAGGCCAAGCCGCCCCCCAAAGCCTGCACACAGCCAAGGCCTAACTATGCCTCGAACTTCAGTG
  TGATCGGGGCGCGGGAGGAGCGGGGGGTCCGCGCACCCAGCTTTGCTCAAAAGCCAAAAGTCTCTGAGAA
  CGACTTTGAAGATCTGTTGTCCAATCAAGGCTTCTCCTCCAGGTCTGACAAGAAAGGGCCAAAGACCATT
  GCAGAGATGAGGAAGCAGGACCTGGCTAAAGACACGGACCCACTCAAGCTGAAGCTCCTGGACTGGATTG
  AGGGCAAGGAGCGGAACATCCGGGCCCTGCTGTCCACGCTGCACACAGTGCTGTGGGACGGGGAGAGCCG
  CTGGACGCCCGTGGGCATGGCCGACCTGGTGGCTCCGGAGCAAGTGAAGAAGCACTATCGCCGCGCGGTG
  CTGGCCGTGCACCCCGACAAGGCTGCGGGGCAGCCGTACGAGCAGCACGCCAAGATGATCTTCATGGAGC
  TGAATGACGCCTGGTCGGAGTTTGAGAACCAGGGCTCCCGGCCCCTCTTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223572 representing NM_005255
  Red=Cloning site Green=Tags(s)
  
  MSLLQSALDFLAGPGSLGGASGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDVGSGREYALKR
  LLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKKMESR
  GPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQR
  RALVEEEITRNTTPMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPP
  HDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQLQEIAAARNVNPKSPITELLEQNGGYGSATLSRGPPPP
  VGPAGSGYSGGLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVM
  SFPAEGVESALKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSRFHNRVSECGWAARRAPHLHTLYNICR
  NMHAWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMC
  DMVAEEPITPHSKPILVRAVVMTPVPLFSKQRSGCRPFCEVYVGDERVASTSQEYDKMRDFKIEDGKAVI
  PLGVTVQGDVLIVIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDL
  FQVNLEVEVEPRDRPSREAPPWENSSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAGAGS
  PEAEPTDSDSPPSSSADASRFLHTLDWQEEKEAETGAENASSKESESALMEDRDESEVSDEGGSPISSEG
  QEPRADPEPPGLAAGLVQQDLVFEVETPAVLPEPVPQEDGVDLLGLHSEVGAGPAVPPQACKAPSSNTDL
  LSCLLGPPEAASQGPPEDLLSEDPLLLASPAPPLSVQSTPRGGPPAAGNNSQPCSNPDLFGEFLNSDSVT
  VPPSFPSAHSAPPPSCSADFLHLGDLPGEPSKMTASSSNPDLLGGWAAWTETAASAVAPTPATEGPLFSP
  GGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASW
  PPQAKPPPKACTQPRPNYASNFSVIGAREERGVRAPSFAQKPKVSENDFEDLLSNQGFSSRSDKKGPKTI
  AEMRKQDLAKDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVGMADLVAPEQVKKHYRRAV
  LAVHPDKAAGQPYEQHAKMIFMELNDAWSEFENQGSRPLF
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005255
• ORF Size: 3900 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005255.1, NP_005246.1
• RefSeq Size: 4331 bp
• RefSeq ORF: 3936 bp
• Locus ID: 2580
• UniProt ID: O14976
• Cytogenetics: 4p16.3
• Domains: pkinase, TyrKc, DnaJ, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]