EHM2 (EPB41L4B) (NM_018424) Human Tagged ORF Clone

CAT#: RG223085

EPB41L4B (tGFP-tagged) - Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 1

Product Images

Circular map for RG223085

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EHM2
• Synonyms: CG1; EHM2; LULU2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223085 representing NM_018424
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGGTTCCTGCGCCGGACCTTTGGCCGCCGCTCCATGCAGCGCTACGCGCGGGGCGCGGCGGGGC
  GCGGGGCCGCCGGGCTGGGGGACGAGCGCGATGGGGGGCCACGGGGGGGCCCGGCCGCCGCCGCCTCCTC
  CTCGGCGCTGCCCGCCGCGCCCGGGGGCAGCGTGTTCCCGGCGGGCGGCGGGCCCCTGCTCACCGGCGGC
  GCGGCCGTGCACATCTCCGCCGCCGGCGCCGCCAAGGCCACCCTCTACTGCCGCGTCTTCCTGCTCGACG
  GGACCGAAGTGAGCGTGGACCTGCCGAAACATGCCAAAGGCCAGGATTTGTTTGATCAGATTGTGTACCA
  CTTGGACCTTGTGGAAACAGATTACTTTGGCCTCCAGTTCCTCGACTCTGCCCAGGTTGCGCACTGGCTG
  GATCATGCCAAACCCATAAAAAAGCAGATGAAAATTGGACCTGCTTATGCTTTACACTTTCGAGTTAAAT
  ACTATTCTTCAGAACCAAACAACCTTCGTGAGGAGTTTACAAGGTACCTGTTTGTTTTACAACTCAGGCA
  TGACATTCTTTCTGGAAAATTGAAATGCCCTTATGAAACAGCTGTGGAATTAGCTGCTCTCTGTCTACAA
  GCGGAGCTTGGGGAGTGCGAGCTTCCAGAACACACACCAGAGCTTGTGTCTGAGTTTCGGTTCATTCCAA
  ATCAGACAGAAGCAATGGAATTTGATATCTTCCAGAGATGGAAAGAGTGCAGGGGAAAGAGCCCTGCCCA
  GGCGGAACTCTCCTATCTGAATAAAGCGAAGTGGCTGGAAATGTATGGGGTAGACATGCACGTTGTCAGG
  GGAAGAGATGGCTGTGAATATTCTCTTGGACTGACCCCGACAGGCATATTAATCTTTGAAGGAGCTAACA
  AAATAGGCTTATTCTTTTGGCCTAAAATTACCAAAATGGATTTTAAAAAGAGCAAATTGACACTCGTGGT
  GGTCGAGGATGATGATCAGGGACGTGAGCAAGAGCACACGTTTGTGTTCCGGTTAGACAGTGCCAGGACC
  TGCAAACACCTTTGGAAGTGTGCAGTTGAGCACCACGCATTCTTCCGACTGCGGACGCCAGGAAACAGCA
  AATCCAATAGATCCGACTTTATCAGGCTGGGCTCTCGCTTCAGATTCAGTGGGCGGACAGAATATCAAGC
  TACACATGGCTCCAGGTTACGAAGAACCAGCACCTTTGAGAGGAAGCCTAGTAAACGTTATCCATCCCGG
  AGACATTCAACGTTCAAAGCAAGCAACCCAGTGATAGCAGCCCAGCTCTGCTCTAAAACAAATCCAGAAG
  TCCATAATTACCAGCCTCAATATCATCCTAATATCCATCCCAGCCAGCCCCGGTGGCATCCTCACTCTCC
  AAATGTCAGGCCATCCTTTCAGGATGACAGGTCGCATTGGAAAGCATCGGCCAGTGGAGATGACAGCCAT
  TTTGATTATGTCCACGACCAGAACCAGAAGAACTTAGGAGGGATGCAAAGTATGATGTATCGAGATAAAC
  TCATGACTGCACTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223085 representing NM_018424
  Red=Cloning site Green=Tags(s)
  
  MLRFLRRTFGRRSMQRYARGAAGRGAAGLGDERDGGPRGGPAAAASSSALPAAPGGSVFPAGGGPLLTGG
  AAVHISAAGAAKATLYCRVFLLDGTEVSVDLPKHAKGQDLFDQIVYHLDLVETDYFGLQFLDSAQVAHWL
  DHAKPIKKQMKIGPAYALHFRVKYYSSEPNNLREEFTRYLFVLQLRHDILSGKLKCPYETAVELAALCLQ
  AELGECELPEHTPELVSEFRFIPNQTEAMEFDIFQRWKECRGKSPAQAELSYLNKAKWLEMYGVDMHVVR
  GRDGCEYSLGLTPTGILIFEGANKIGLFFWPKITKMDFKKSKLTLVVVEDDDQGREQEHTFVFRLDSART
  CKHLWKCAVEHHAFFRLRTPGNSKSNRSDFIRLGSRFRFSGRTEYQATHGSRLRRTSTFERKPSKRYPSR
  RHSTFKASNPVIAAQLCSKTNPEVHNYQPQYHPNIHPSQPRWHPHSPNVRPSFQDDRSHWKASASGDDSH
  FDYVHDQNQKNLGGMQSMMYRDKLMTAL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018424
• ORF Size: 1554 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018424.3, NP_060894.2
• RefSeq Size: 3783 bp
• RefSeq ORF: 1557 bp
• Locus ID: 54566
• UniProt ID: Q9H329
• Cytogenetics: 9q31.3
• Gene Summary: Up-regulates the activity of the Rho guanine nucleotide exchange factor ARHGEF18 (By similarity). Involved in the regulation of the circumferential actomyosin belt in epithelial cells (PubMed:22006950). Promotes cellular adhesion, migration and motility in vitro and may play a role in wound healing (PubMed:23664528). May have a role in mediating cytoskeletal changes associated with steroid-induced cell differentiation (PubMed:14521927).[UniProtKB/Swiss-Prot Function]