EHM2 (EPB41L4B) (NM_018424) Human Untagged Clone

CAT#: SC317872

EPB41L4B (untagged)-Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EHM2
• Synonyms: CG1; EHM2; LULU2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317872 representing NM_018424.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGCGGTTCCTGCGCCGGACCTTTGGCCGCCGCTCCATGCAGCGCTACGCGCGGGGCGCGGCGGGG
  CGCGGGGCCGCCGGGCTGGGGGACGAGCGCGATGGGGGGCCACGGGGGGGCCCGGCCGCCGCCGCCTCC
  TCCTCGGCGCTGCCCGCCGCGCCCGGGGGCAGCGTGTTCCCGGCGGGCGGCGGGCCCCTGCTCACCGGC
  GGCGCGGCCGTGCACATCTCCGCCGCCGGCGCCGCCAAGGCCACCCTCTACTGCCGCGTCTTCCTGCTC
  GACGGGACCGAAGTGAGCGTGGACCTGCCGAAACATGCCAAAGGCCAGGATTTGTTTGATCAGATTGTG
  TACCACTTGGACCTTGTGGAAACAGATTACTTTGGCCTCCAGTTCCTCGACTCTGCCCAGGTTGCGCAC
  TGGCTGGATCATGCCAAACCCATAAAAAAGCAGATGAAAATTGGACCTGCTTATGCTTTACACTTTCGA
  GTTAAATACTATTCTTCAGAACCAAACAACCTTCGTGAGGAGTTTACAAGGTACCTGTTTGTTTTACAA
  CTCAGGCATGACATTCTTTCTGGAAAATTGAAATGCCCTTATGAAACAGCTGTGGAATTAGCTGCTCTC
  TGTCTACAAGCGGAGCTTGGGGAGTGCGAGCTTCCAGAACACACACCAGAGCTTGTGTCTGAGTTTCGG
  TTCATTCCAAATCAGACAGAAGCAATGGAATTTGATATCTTCCAGAGATGGAAAGAGTGCAGGGGAAAG
  AGCCCTGCCCAGGCGGAACTCTCCTATCTGAATAAAGCGAAGTGGCTGGAAATGTATGGGGTAGACATG
  CACGTTGTCAGGGGAAGAGATGGCTGTGAATATTCTCTTGGACTGACCCCGACAGGCATATTAATCTTT
  GAAGGAGCTAACAAAATAGGCTTATTCTTTTGGCCTAAAATTACCAAAATGGATTTTAAAAAGAGCAAA
  TTGACACTCGTGGTGGTCGAGGATGATGATCAGGGACGTGAGCAAGAGCACACGTTTGTGTTCCGGTTA
  GACAGTGCCAGGACCTGCAAACACCTTTGGAAGTGTGCAGTTGAGCACCACGCATTCTTCCGACTGCGG
  ACGCCAGGAAACAGCAAATCCAATAGATCCGACTTTATCAGGCTGGGCTCTCGCTTCAGATTCAGTGGG
  CGGACAGAATATCAAGCTACACATGGCTCCAGGTTACGAAGAACCAGCACCTTTGAGAGGAAGCCTAGT
  AAACGTTATCCATCCCGGAGACATTCAACGTTCAAAGCAAGCAACCCAGTGATAGCAGCCCAGCTCTGC
  TCTAAAACAAATCCAGAAGTCCATAATTACCAGCCTCAATATCATCCTAATATCCATCCCAGCCAGCCC
  CGGTGGCATCCTCACTCTCCAAATGTCAGGCCATCCTTTCAGGATGACAGGTCGCATTGGAAAGCATCG
  GCCAGTGGAGATGACAGCCATTTTGATTATGTCCACGACCAGAACCAGAAGAACTTAGGAGGGATGCAA
  AGTATGATGTATCGAGATAAACTCATGACTGCACTTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_018424
• Insert Size: 1557 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018424.3
• RefSeq Size: 4030 bp
• RefSeq ORF: 1557 bp
• Locus ID: 54566
• UniProt ID: Q9H329
• Cytogenetics: 9q31.3
• MW: 58.6 kDa
• Gene Summary: Up-regulates the activity of the Rho guanine nucleotide exchange factor ARHGEF18 (By similarity). Involved in the regulation of the circumferential actomyosin belt in epithelial cells (PubMed:22006950). Promotes cellular adhesion, migration and motility in vitro and may play a role in wound healing (PubMed:23664528). May have a role in mediating cytoskeletal changes associated with steroid-induced cell differentiation (PubMed:14521927).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) lacks several exons and includes an alternate 3' terminal exon, compared to variant 1. It encodes isoform 1 which is shorter and has a distinct C-terminus, compared to isoform 2.