USP33 (NM_015017) Human Tagged ORF Clone

CAT#: RG222966

USP33 (tGFP-tagged) - Human ubiquitin specific peptidase 33 (USP33), transcript variant 1

Product Images

Circular map for RG222966

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: USP33
• Synonyms: VDU1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222966 representing NM_015017
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGGATCAAATTCACACATAACGATATTAACCTTAAAGGTGTTACCTCATTTTGAAAGTCTTGGGA
  AACAGGAAAAAATTCCTAACAAAATGTCAGCTTTTCGAAATCATTGTCCACATTTGGATTCAGTTGGTGA
  AATAACAAAAGAAGATTTGATACAAAAATCCCTTGGTACTTGTCAGGATTGTAAAGTCCAAGGACCAAAT
  CTTTGGGCATGTCTGGAGAATAGATGTTCATATGTTGGCTGTGGTGAATCACAAGTAGATCACAGCACCA
  TACATTCTCAGGAGACAAAGCATTATCTAACTGTGAACCTTACCACTCTTCGAGTATGGTGTTATGCTTG
  CAGCAAAGAAGTATTTTTGGATAGGAAATTAGGAACTCAGCCTTCATTGCCTCATGTAAGACAACCTCAC
  CAAATACAAGAAAACAGTGTCCAGGATTTTAAAATACCCAGTAATACAACATTAAAAACTCCTCTGGTTG
  CCGTATTTGATGATCTGGATATAGAAGCGGATGAAGAAGATGAACTTAGGGCCAGAGGTCTTACAGGTTT
  GAAAAATATTGGAAATACTTGTTACATGAATGCAGCTTTGCAGGCTCTTTCTAATTGCCCACCTTTGACA
  CAGTTTTTTCTTGATTGTGGAGGACTAGCTCGAACAGATAAGAAACCTGCCATTTGTAAAAGTTATCTCA
  AACTAATGACAGAGCTGTGGCATAAAAGCAGGCCAGGATCTGTTGTGCCTACTACTCTGTTTCAAGGAAT
  TAAAACTGTAAATCCAACATTTCGGGGGTATTCTCAGCAGGATGCTCAAGAATTCCTTCGATGTTTAATG
  GATTTGCTTCATGAAGAATTGAAAGAGCAAGTCATGGAAGTAGAAGAAGATCCGCAAACCATAACCACTG
  AGGAGACAATGGAAGAAGACAAGAGCCAGTCGGATGTAGATTTTCAGTCTTGTGAATCTTGTAGCAACAG
  TGATAGAGCAGAAAATGAAAATGGCTCTAGATGCTTTTCTGAAGATAATAATGAAACAACAATGTTAATT
  CAGGATGATGAAAACAATTCAGAAATGTCAAAGGATTGGCAAAAAGAGAAGATGTGCAATAAGATTAATA
  AAGTAAATTCTGAAGGCGAATTTGATAAAGATAGAGACTCTATATCTGAAACAGTCGACTTAAACAACCA
  GGAAACTGTCAAAGTGCAAATACACAGCAGAGCTTCAGAATATATCACTGATGTCCATTCGAATGACCTG
  TCTACACCACAGATCCTTCCATCAAATGAAGGTGTTAATCCACGTTTATCGGCAAGCCCTCCTAAATCAG
  GCAATTTGTGGCCAGGATTGGCACCACCACACAAAAAAGCTCAGTCTGCATCTCCAAAGAGAAAAAAACA
  GCACAAGAAATACAGAAGTGTTATTTCAGACATATTTGATGGAACAATCATTAGTTCAGTGCAGTGTCTG
  ACTTGTGACAGGGTGTCTGTAACCCTCGAGACCTTTCAAGATCTGTCCTTGCCAATTCCTGGCAAGGAAG
  ACCTTGCTAAGCTGCATTCATCAAGTCATCCAACTTCTATAGTCAAAGCAGGATCATGTGGCGAAGCATA
  TGCTCCACAAGGGTGGATAGCTTTTTTCATGGAATATGTGAAGAGGTTTGTTGTCTCATGTGTCCCTAGC
  TGGTTTTGGGGTCCAGTAGTAACCTTGCAAGATTGTCTTGCTGCCTTCTTTGCCAGAGATGAACTAAAAG
  GTGACAATATGTACAGTTGTGAAAAATGCAAAAAGTTGAGAAATGGAGTGAAGTTTTGTAAAGTACAAAA
  CTTTCCTGAGATTTTGTGCATCCACCTTAAAAGATTCAGACATGAACTAATGTTTTCCACCAAAATCAGT
  ACCCATGTTTCATTTCCGCTAGAAGGCTTGGATCTTCAGCCATTTCTTGCTAAGGATAGTCCAGCTCAAA
  TTGTGACATATGATCTTCTGTCAGTCATTTGCCATCATGGAACTGCAAGTAGTGGACACTATATAGCCTA
  CTGCCGAAACAATCTAAATAATCTCTGGTATGAATTTGATGATCAGAGTGTCACTGAAGTTTCAGAATCT
  ACTGTACAAAATGCAGAAGCTTACGTTCTTTTCTATAGGAAGAGCAGCGAAGAGGCACAAAAAGAGAGGA
  GAAGGATATCAAATTTATTGAACATAATGGAACCAAGCCTCCTTCAGTTTTATATTTCTCGACAGTGGCT
  TAATAAATTTAAGACCTTTGCCGAACCTGGCCCTATTTCAAATAATGACTTTCTTTGTATTCATGGAGGT
  GTTCCTCCAAGAAAAGCTGGTTATATTGAAGACCTGGTTTTGATGCTGCCTCAGAACATTTGGGATAACC
  TATATAGCAGGTATGGTGGAGGACCAGCTGTCAACCATCTGTACATTTGTCATACTTGCCAAATTGAGGC
  GGAGAAAATTGAAAAAAGAAGAAAAACTGAATTGGAAATTTTTATTCGGCTTAACAGAGCGTTCCAAAAA
  GAGGACTCTCCAGCTACTTTTTATTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGG
  GTAAAGATGGAGATCCTCCAGGTCCTATTGACAATACTAAGATTGCAGTCACTAAATGTGGTAATGTGAT
  GCTTAGGCAAGGAGCAGATTCTGGCCAGATTTCTGAAGAAACATGGAATTTTCTGCAGTCTATTTATGGT
  GGAGGGCCTGAAGTTATCCTGCGACCTCCGGTTGTTCATGTTGATCCAGATATACTTCAAGCAGAAGAAA
  AAATTGAAGTAGAAACTCGGTCTTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222966 representing NM_015017
  Red=Cloning site Green=Tags(s)
  
  MTGSNSHITILTLKVLPHFESLGKQEKIPNKMSAFRNHCPHLDSVGEITKEDLIQKSLGTCQDCKVQGPN
  LWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTQPSLPHVRQPH
  QIQENSVQDFKIPSNTTLKTPLVAVFDDLDIEADEEDELRARGLTGLKNIGNTCYMNAALQALSNCPPLT
  QFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPTTLFQGIKTVNPTFRGYSQQDAQEFLRCLM
  DLLHEELKEQVMEVEEDPQTITTEETMEEDKSQSDVDFQSCESCSNSDRAENENGSRCFSEDNNETTMLI
  QDDENNSEMSKDWQKEKMCNKINKVNSEGEFDKDRDSISETVDLNNQETVKVQIHSRASEYITDVHSNDL
  STPQILPSNEGVNPRLSASPPKSGNLWPGLAPPHKKAQSASPKRKKQHKKYRSVISDIFDGTIISSVQCL
  TCDRVSVTLETFQDLSLPIPGKEDLAKLHSSSHPTSIVKAGSCGEAYAPQGWIAFFMEYVKRFVVSCVPS
  WFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQNFPEILCIHLKRFRHELMFSTKIS
  THVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSES
  TVQNAEAYVLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGG
  VPPRKAGYIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIEAEKIEKRRKTELEIFIRLNRAFQK
  EDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGNVMLRQGADSGQISEETWNFLQSIYG
  GGPEVILRPPVVHVDPDILQAEEKIEVETRSL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015017
• ORF Size: 2826 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015017.5
• RefSeq Size: 4524 bp
• RefSeq ORF: 2829 bp
• Locus ID: 23032
• UniProt ID: Q8TEY7
• Cytogenetics: 1p31.1
• Domains: UCH, zf-UBP, DUSP
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]