USP33 (NM_015017) Human Untagged Clone

CAT#: SC127627

USP33 (untagged)-Human ubiquitin specific peptidase 33 (USP33), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP33
• Synonyms: VDU1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127627 sequence for NM_015017 edited (data generated by NextGen Sequencing)
  ATGACAGGATCAAATTCACACATAACGATATTAACCTTAAAGGTGTTACCTCATTTTGAA
  AGTCTTGGGAAACAGGAAAAAATTCCTAACAAAATGTCAGCTTTTCGAAATCATTGTCCA
  CATTTGGATTCAGTTGGTGAAATAACAAAAGAAGATTTGATACAAAAATCCCTTGGTACT
  TGTCAGGATTGTAAAGTCCAAGGACCAAATCTTTGGGCATGTCTGGAGAATAGATGTTCA
  TATGTTGGCTGTGGTGAATCACAAGTAGATCACAGCACCATACATTCTCAGGAGACAAAG
  CATTATCTAACTGTGAACCTTACCACTCTTCGAGTATGGTGTTATGCTTGCAGCAAAGAA
  GTATTTTTGGATAGGAAATTAGGAACTCAGCCTTCATTGCCTCATGTAAGACAACCTCAC
  CAAATACAAGAAAACAGTGTCCAGGATTTTAAAATACCCAGTAATACAACATTAAAAACT
  CCTCTGGTTGCCGTATTTGATGATCTGGATATAGAAGCGGATGAAGAAGATGAACTTAGG
  GCMAGAGGTCTTACAGGTTTGAAAAATATTGGAAATACTTGTTACATGAATGCAGCTTTG
  CAGGCTCTTTCTAATTGCCCACCTTTGACACAGTTTTTTCTTGATTGTGGAGGACTAGCT
  CGAACAGATAAGAAACCTGCCATTTGTAAAAGTTATCTCAAACTAATGACAGAGCTGTGG
  CATAAAAGCAGGCCAGGATCTGTTGTGCCTACTACTCTGTTTCAAGGAATTAAAACTGTA
  AATCCAACATTTCGGGGGTATTCTCAGCAGGATGCTCAAGAATTCCTTCGATGTTTAATG
  GATTTGCTTCATGAAGAATTGAAAGAGCAAGTCATGGAAGTAGAAGAAGATCCGCAAACC
  ATAACCACTGAGGAGACAATGGAAGAAGACAAGAGCCAGTCGGATGTAGATTTTCAGTCT
  TGTGAATCTTGTAGCAACAGTGATAGAGCAGAAAATGAAAATGGCTCTAGATGCTTTTCT
  GAAGATAATAATGAAACAACAATGTTAATTCAGGATGATGAAAACAATTCAGAAATGTCA
  AAGGATTGGCAAAAAGAGAAGATGTGCAATAAGATTAATAAAGTAAATTCTGAAGGCGAA
  TTTGATAAAGATAGAGACTCTATATCTGAAACAGTCGACTTAAACAACCAGGAAACTGTC
  AAAGTGCAAATACACAGCAGAGCTTCAGAATATATCACTGATGTCCATTCGAATGACCTG
  TCTACACCACAGATCCTTCCATCAAATGAAGGTGTTAATCCACGTTTATCGGCAAGCCCT
  CCTAAATCAGGCAATTTGTGGCCAGGATTGGCACCACCACACAAAAAAGCTCAGTCTGCA
  TCTCCAAAGAGAAAAAAACAGCACAAGAAATACAGAAGTGTTATTTCAGACATATTTGAT
  GGAACAATCATTAGTTCAGTGCAGTGTCTGACTTGTGACAGGGTGTCTGTAACCCTCGAG
  ACCTTTCAAGATCTGTCCTTGCCAATTCCTGGCAAGGAAGACCTTGCTAAGCTGCATTCA
  TCAAGTCATCCAACTTCTATAGTCAAAGCAGGATCATGTGGCGAAGCATATGCTCCACAA
  GGGTGGATAGCTTTTTTCATGGAATATGTGAAGAGGTTTGTTGTCTCATGTGTCCCTAGC
  TGGTTTTGGGGTCCAGTAGTAACCTTGCAAGATTGTCTTGCTGCCTTCTTTGCCAGAGAT
  GAACTAAAAGGTGACAATATGTACAGTTGTGAAAAATGCAAAAAGYTGAGAAATGGAGTG
  AAGTTTTGTAAAGTACAAAACTTTCCTGAGATTTTGTGCATCCACCTTAAAAGATTCAGA
  CATGAACTAATGTTTTCCACCAAAATCAGTACCCATGTTTCATTTCCGCTAGAAGGCTTG
  GATCTTCAGCCATTTCTTGCTAAGGATAGTCCAGCTCAAATTGTGACATATGATCTTCTG
  TCAGTCATTTGCCATCATGGAACTGCAAGTAGTGGACACTATATAGCCTACTGCCGAAAC
  AATCTAAATAATCTCTGGTATGAATTTGATGATCAGAGTGTCACTGAAGTTTCAGAATCT
  ACTGTACAAAATGCAGAAGCTTACGTTCTTTTCTATAGGAAGAGCAGCGAAGAGGCACAA
  AAAGAGAGGAGAAGGATATCAAATTTATTGAACATAATGGAACCAAGCCTCCTTCAGTTT
  TATATTTCTCGACAGTGGCTTAATAAATTTAAGACCTTTGCCGAACCTGGCCCTATTTCA
  AATAATGACTTTCTTTGTATTCATGGAGGTGTTCCTCCAAGAAAAGCTGGTTATATTGAA
  GACCTGGTTTTGATGCTGCCTCAGAACATTTGGGATAACCTATATAGCAGGTATGGTGGA
  GGACCAGCTGTCAACCATCTGTACATTTGTCATACTTGCCAAATTGAGGCGGAGAAAATT
  GAAAAAAGAAGAAAAACTGAATTGGAAATTTTTATTCGGCTTAACAGAGCGTTCCAAAAA
  GAGGACTCTCCAGCTACTTTTTATTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGT
  TTTGTGAAGGGTAAAGATGGAGATCCTCCAGGTCCTATTGACAATACTAAGATTGCAGTC
  ACTAAATGTGGTAATGTGATGCTTAGGCAAGGAGCAGATTCTGGCCAGATTTCTGAAGAA
  ACATGGAATTTTCTGCAGTCTATTTATGGTGGAGGGCCTGAAGTTATCCTGCGACCTCCG
  GTTGTTCATGTTGATCCAGATATACTTCAAGCAGAAGAAAAAATTGAAGTAGAAACTCGG
  TCTTTGTAA
  
  Clone variation with respect to NM_015017.3
  543 c=>m;1786 t=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015017 unedited
  TTTTGTATACGACCACTATAGGGCGGCCGCGATTCGGCACGAGGGCCAGTTCAGGAGGCG
  GACCCCCCGAGGCAGCGCTGCGGGGCCGTTTTCCGGCCCTCCTGACGCGACACTGCCCCT
  CTCCGAGAGCTGAGAAGGAAAAGAGGAGCTTGCGGAGGTGCGGCTGCAGGCCGTTGTTGG
  TCGAGCTGGCGGGTCCCGCGGGCCAGGCCGTGGAGAGCTCCTGAAGGAAGCACTAAACAT
  GGAAAGGAACAACCGGTACCAGCCACTGCAAAAACATGCCAAATTGTAAAGACCATCGAT
  GTTAGGAAGAAACTGCCTCAACTAATGAGCAAAATAACCAGCTAACATCGTAATGACAGG
  ATCAAATTCACACATAACGATATTAACCTTAAAGGTGTTACCTCATTTTGAAAGTCTTGG
  GAAACAGGAAAAAATTCCTAACAAAATGTCAGCTTTTCGAAATCATTGTCCACATTTGGA
  TTCAGTTGGTGAAATAACAAAAGAAGATTTGATACAAAAATCCCTTGGTACTTGTCAGGA
  TTGTAAAGTCCAAGGACCAAATCTTTGGGCATGTCTGGAGAATAGATGTTCATATGTTGG
  CTGTGGTGAATCACAAGTAGATCACAGCACCATACATTCTCAGGAGACAAAGCATTATCT
  AACTGTGAACCTTACCACTCTTCGAGTATGGTGTTATGCTTGCAGCANAGAAGTATTTTT
  GGATAGGAAATTANGAACTCAGCCTTCATTGCCTCATGNTAGACAACCTCACCAAATACA
  AGAAAACAGTGTCCAGNATTTTTAAATACCCCAGTATACAACATTTANAACTCCTCTGGN
  TGCCGTATTTGATGATCTGGATATAGAAGCGGATGAAGAAGATGAACTTAGGNNCCAGAG
  TAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015017 unedited
  TTGACATGGCATGGCACTGTCCAGGNCCAGGANAGCACTGGGGNAGGGTCACAGGGATGC
  CACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTT
  TTTTTTTTTTTTTTTTACATTTTAAAGACATTTTTATTGAGCTAATTTTAACAACATTGC
  TTTAGCTGGTGACAGCTGCCCCAAACCAAAACAAAGCCATCATGAATGCTATTCAACATC
  CTCAATGTAATCCAGTATGTTTTTGTACTTGGAATATAGTTAAACTTTTGACATTACATA
  ATCAAGCAAATAGCAGTGCATACTATATTATTCAAAAAGACTTTATCTATTTCATTTAAA
  AAATCAAGTTGCAAGTGGCCTCAGCTTTATCAACAATCGTAGTGACACATTCCACACTTC
  ATGCTCTCAAAATAAAAAGTGCCCTAAAACTAACTCTAAGTTTTTTAGTCACTGACATTA
  ATACTAACCAGGTTACAGGAATTGAAGTTTAACATTGTACAATATAAGCGGCAATAAGTT
  ACTGATATCTGCTGACAAATTCCACTCAAACTAAATATATCCTTGATACATTCAAAAAGA
  CATTTTGTAAATTTTAATAAGCTAGTTAGTGTATATGCTGCAGTGCAAAAAAAGTGACAC
  CCTAGTTTCCTGAGCTTTAGGAAATTAACAATTTTCTGACTACTACTTTCAACTTAAGAT
  ACGCTCTTTATTATATGAAGCCCTGACANAACTATACAATTAAATTGCGCCTTTTGGAAG
  ACTGAAAGACTGGTCAGAGTCAGGGTGATTTAGGCACAATGAAAAGTTTTCCAAAGCAAA
  TGTATTTACACCCCTCAAAGGATACTATGGATCTGCCTGTTGTGGAAAAATTAAAGATAA
  CCTCTTGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015017
• Insert Size: 5200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015017.3, NP_055832.3
• RefSeq Size: 4524 bp
• RefSeq ORF: 2829 bp
• Locus ID: 23032
• UniProt ID: Q8TEY7
• Cytogenetics: 1p31.1
• Domains: UCH, zf-UBP, DUSP
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).