GRIK1 (NM_000830) Human Tagged ORF Clone

CAT#: RG222898

GRIK1 (tGFP-tagged) - Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1

Product Images

Circular map for RG222898

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GRIK1
• Synonyms: EAA3; EEA3; GLR5; GluK1; gluR-5; GLUR5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222898 representing NM_000830
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCACGGCACACTCCTCGCCCAGCCCGGGCTCTGGACCAGGGACACCAGCTGGGCACTCCTCTATT
  TCCTCTGCTATATCCTCCCTCAGACCGCCCCGCAAGTACTCAGGATCGGAGGGATTTTTGAAACAGTGGA
  AAATGAGCCTGTTAATGTTGAAGAATTAGCTTTCAAGTTTGCAGTCACCAGCATTAACAGAAACCGAACC
  CTGATGCCTAACACCACATTAACCTATGACATCCAGAGAATTAACCTTTTTGATAGTTTTGAAGCCTCGC
  GGAGAGCATGTGACCAGCTGGCTCTTGGTGTGGCTGCTCTCTTTGGCCCTTCCCATAGCTCCTCCGTCAG
  TGCTGTGCAGTCTATTTGCAATGCTCTCGAAGTTCCACACATACAGACCCGCTGGAAACACCCCTCGGTG
  GACAACAAAGATTTGTTTTACATCAACCTTTACCCAGATTATGCAGCTATCAGCAGGGCGATCCTGGATC
  TGGTCCTCTATTACAACTGGAAAACAGTGACAGTGGTGTATGAAGACAGCACAGGTCTAATTCGTCTACA
  AGAGCTCATCAAAGCTCCCTCCAGATATAATATTAAAATCAAAATCCGCCAGCTGCCCTCTGGGAATAAA
  GATGCCAAGCCTTTACTCAAGGAGATGAAGAAAGGCAAGGAGTTCTATGTGATATTTGATTGTTCACATG
  AAACAGCCGCTGAAATCCTTAAGCAGATTCTGTTCATGGGCATGATGACCGAGTACTATCACTACTTTTT
  CACAACCCTGGACTTATTTGCTTTGGATCTGGAACTCTATAGGTACAGTGGCGTAAACATGACCGGGTTT
  CGGCTGCTTAACATTGACAACCCTCACGTGTCATCCATCATTGAGAAGTGGTCCATGGAGAGACTGCAGG
  CCCCACCCAGGCCCGAGACTGGCCTTTTGGATGGCATGATGACAACTGAAGCGGCTCTGATGTACGATGC
  TGTGTACATGGTGGCCATTGCCTCGCACCGGGCATCCCAGCTGACCGTCAGCTCCCTGCAGTGCCATAGA
  CATAAGCCATGGCGCCTCGGACCCAGATTTATGAACCTGATCAAAGAGGCCCGGTGGGATGGCTTGACTG
  GGCATATCACCTTTAATAAAACCAATGGCTTGAGGAAGGATTTTGATCTGGACATTATTAGTCTCAAAGA
  GGAAGGAACTGAAAAGGCTGCTGGCGAAGTGTCTAAACACTTGTATAAAGTGTGGAAGAAGATTGGGATT
  TGGAATTCCAACAGTGGGCTTAACATGACGGACAGCAACAAAGACAAGTCCAGCAATATCACTGATTCAT
  TGGCCAACAGAACACTCATTGTCACCACCATTCTGGAAGAACCCTATGTTATGTACAGGAAATCTGATAA
  GCCTCTATATGGAAATGACAGATTTGAAGGATATTGCCTAGACCTGTTGAAAGAATTGTCAAACATCCTG
  GGTTTCATTTATGATGTTAAACTAGTTCCCGATGGCAAATATGGGGCCCAGAATGACAAAGGGGAGTGGA
  ACGGGATGGTTAAAGAACTCATAGATCACAGGGCTGACCTGGCAGTGGCTCCTCTTACCATCACCTACGT
  GCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCCTAGGCATCAGCATTCTCTACCGGAAGCCC
  AATGGTACCAATCCAGGCGTTTTCTCCTTCCTCAACCCCCTGTCTCCAGATATTTGGATGTATGTGCTCT
  TAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTGATTGCAAGGTTTACACCCTACGAGTGGTATAACCC
  CCACCCATGCAACCCTGACTCAGACGTGGTGGAAAACAATTTTACTTTACTAAATAGTTTCTGGTTTGGA
  GTTGGAGCTCTCATGCAGCAAGGATCAGAGCTGATGCCCAAAGCTCTATCGACCAGAATAGTTGGAGGGA
  TATGGTGGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAATCTGGCTGCCTTCTTGACAGTAGA
  GAGAATGGAATCCCCCATAGATTCGGCAGATGATCTGGCAAAGCAAACCAAGATAGAATATGGGGCGGTT
  AGAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCCACCTATGAGAAGATGTGGGCTTTCA
  TGAGCAGCAGGCAGCAGACCGCCCTGGTAAGAAACAGTGATGAGGGGATCCAGAGAGTGCTCACCACAGA
  CTACGCGCTGCTGATGGAGTCCACCAGCATTGAGTATGTGACGCAGAGAAACTGCAACCTCACTCAGATC
  GGGGGCCTCATTGACTCCAAAGGTTACGGAGTGGGAACACCTATTGGTTCTCCTTACCGGGATAAAATTA
  CTATTGCTATTCTTCAACTCCAAGAAGAAGGGAAGCTGCATATGATGAAAGAGAAGTGGTGGCGTGGGAA
  TGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGTGGAAAATATTGGAGGCATCTTCATT
  GTTCTGGCTGCCGGACTGGTCCTTTCTGTATTTGTAGCTATTGGAGAATTCATATACAAATCACGGAAGA
  ATAATGATATTGAACAGGCTTTTTGTTTCTTTTATGGACTGCAATGTAAGCAAACCCATCCAACCAACTC
  CACTTCTGGAACTACTTTATCTACGGATTTAGAATGTGGTAAATTAATTCGAGAGGAGAGAGGGATTCGA
  AAACAGTCCTCAGTTCATACTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222898 representing NM_000830
  Red=Cloning site Green=Tags(s)
  
  MEHGTLLAQPGLWTRDTSWALLYFLCYILPQTAPQVLRIGGIFETVENEPVNVEELAFKFAVTSINRNRT
  LMPNTTLTYDIQRINLFDSFEASRRACDQLALGVAALFGPSHSSSVSAVQSICNALEVPHIQTRWKHPSV
  DNKDLFYINLYPDYAAISRAILDLVLYYNWKTVTVVYEDSTGLIRLQELIKAPSRYNIKIKIRQLPSGNK
  DAKPLLKEMKKGKEFYVIFDCSHETAAEILKQILFMGMMTEYYHYFFTTLDLFALDLELYRYSGVNMTGF
  RLLNIDNPHVSSIIEKWSMERLQAPPRPETGLLDGMMTTEAALMYDAVYMVAIASHRASQLTVSSLQCHR
  HKPWRLGPRFMNLIKEARWDGLTGHITFNKTNGLRKDFDLDIISLKEEGTEKAAGEVSKHLYKVWKKIGI
  WNSNSGLNMTDSNKDKSSNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNIL
  GFIYDVKLVPDGKYGAQNDKGEWNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKP
  NGTNPGVFSFLNPLSPDIWMYVLLACLGVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNFTLLNSFWFG
  VGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAV
  RDGSTMTFFKKSKISTYEKMWAFMSSRQQTALVRNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNLTQI
  GGLIDSKGYGVGTPIGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDNKEASALGVENIGGIFI
  VLAAGLVLSVFVAIGEFIYKSRKNNDIEQAFCFFYGLQCKQTHPTNSTSGTTLSTDLECGKLIREERGIR
  KQSSVHTV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000830
• ORF Size: 2754 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000830.5
• RefSeq Size: 3231 bp
• RefSeq ORF: 2757 bp
• Locus ID: 2897
• UniProt ID: P39086
• Cytogenetics: 21q21.3
• Domains: lig_chan, ANF_receptor
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008]