MTGR1 (CBFA2T2) (NM_005093) Human Tagged ORF Clone

CAT#: RG222716

CBFA2T2 (tGFP-tagged) - Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 2

Product Images

Circular map for RG222716

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MTGR1
• Synonyms: EHT; MTGR1; p85; ZMYND3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222716 representing NM_005093
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAAAGAATCTGGAATAAGCTTGAAAGAAATACAGGTCCTGGCAAGGCAATGGAAAGTTGGTCCTG
  AGAAAAGGGTGCCAGCGATGCCTGGATCGCCTGTGGAAGTGAAGATACAGTCCAGATCCTCACCTCCCAC
  CATGCCACCCCTCCCACCAATAAATCCTGGAGGACCGAGGCCAGTGTCCTTCACTCCTACTGCATTAAGC
  AATGGCATCAACCATTCTCCTCCTACCCTGAATGGTGCCCCATCACCGCCACAGAGATTCAGCAATGGTC
  CTGCCTCCTCCACATCATCTGCACTCACAAATCAGCAATTGCCAGCCACTTGTGGTGCTCGACAACTCAG
  CAAGTTGAAACGCTTTCTTACCACTCTGCAACAGTTTGGCAATGACATCTCCCCTGAGATTGGGGAGAAG
  GTGCGGACTCTTGTTCTTGCACTGGTGAACTCAACAGTGACAATTGAGGAATTCCACTGTAAGCTCCAAG
  AAGCCACAAACTTTCCCCTTCGTCCTTTTGTGATTCCATTTCTCAAGGCCAACCTGCCCCTGCTGCAGCG
  GGAACTGCTGCACTGCGCTCGGGCGGCCAAGCAGACCCCATCCCAGTACCTGGCTCAGCACGAACACCTT
  CTGCTCAACACAAGCATTGCATCGCCTGCTGACTCGTCAGAGTTGCTCATGGAGGTGCACGGAAATGGGA
  AGAGGCCCAGTCCAGAGAGGAGAGAAGAGAATAGTTTTGATAGAGACACAATTGCTCCTGAGCCTCCTGC
  CAAGAGAGTATGTACCATCAGCCCTGCTCCTCGGCACAGTCCTGCTCTCACTGTGCCCCTCATGAATCCC
  GGGGGCCAATTCCATCCTACCCCTCCACCTCTTCAGCATTACACCTTAGAGGATATTGCAACTTCTCACC
  TGTATCGGGAACCCAACAAGATGCTAGAGCATCGAGAAGTTCGTGATAGACACCACAGTCTTGGTCTAAA
  TGGAGGCTATCAAGATGAGTTGGTAGATCATCGTTTGACAGAAAGGGAATGGGCTGATGAATGGAAACAT
  CTTGACCATGCGCTGAATTGCATTATGGAAATGGTAGAGAAAACAAGGCGCTCTATGGCAGTTCTGCGGC
  GCTGTCAGGAATCAGATCGTGAAGAACTCAACTACTGGAAAAGACGGTACAATGAAAACACAGAGCTGAG
  GAAAACGGGGACCGAGTTGGTCTCCAGGCAGCACAGCCCTGGGAGTGCAGATTCTCTCAGCAATGATTCT
  CAGAGAGAGTTCAACAGCAGGCCAGGTACAGGATACGTACCTGTGGAGTTTTGGAAAAAAACAGAAGAAG
  CTGTGAATAAGGTGAAAATTCAGGCCATGTCAGAAGTACAGAAGGCCGTCGCTGAGGCAGAGCAGAAAGC
  CTTTGAAGTGATTGCAACAGAGAGAGCACGAATGGAGCAAACCATAGCGGATGTCAAGCGGCAGGCCGCA
  GAGGATGCTTTCCTCGTCATCAATGAGCAAGAGGAGTCCACGGAGAACTGCTGGAACTGTGGCCGCAAAG
  CCAGCGAGACATGCAGTGGCTGCAATATCGCGCGATACTGTGGCTCTTTCTGCCAGCACAAGGACTGGGA
  GCGGCACCACCGCCTCTGTGGTCAGAACCTGCATGGCCAGAGCCCCCACGGCCAGGGCCGGCCGCTGCTT
  CCTGTAGGCAGGGGCTCCTCTGCCAGGTCCGCCGACTGCAGCGTGCCCAGCCCAGCCCTCGACAAGACCT
  CGGCAACCACATCGCGTTCCTCAACACCTGCTTCTGTGACAGCTATCGACACCAACGGACTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222716 representing NM_005093
  Red=Cloning site Green=Tags(s)
  
  MAKESGISLKEIQVLARQWKVGPEKRVPAMPGSPVEVKIQSRSSPPTMPPLPPINPGGPRPVSFTPTALS
  NGINHSPPTLNGAPSPPQRFSNGPASSTSSALTNQQLPATCGARQLSKLKRFLTTLQQFGNDISPEIGEK
  VRTLVLALVNSTVTIEEFHCKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARAAKQTPSQYLAQHEHL
  LLNTSIASPADSSELLMEVHGNGKRPSPERREENSFDRDTIAPEPPAKRVCTISPAPRHSPALTVPLMNP
  GGQFHPTPPPLQHYTLEDIATSHLYREPNKMLEHREVRDRHHSLGLNGGYQDELVDHRLTEREWADEWKH
  LDHALNCIMEMVEKTRRSMAVLRRCQESDREELNYWKRRYNENTELRKTGTELVSRQHSPGSADSLSNDS
  QREFNSRPGTGYVPVEFWKKTEEAVNKVKIQAMSEVQKAVAEAEQKAFEVIATERARMEQTIADVKRQAA
  EDAFLVINEQEESTENCWNCGRKASETCSGCNIARYCGSFCQHKDWERHHRLCGQNLHGQSPHGQGRPLL
  PVGRGSSARSADCSVPSPALDKTSATTSRSSTPASVTAIDTNGL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005093
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005093.3
• RefSeq Size: 6920 bp
• RefSeq ORF: 1815 bp
• Locus ID: 9139
• UniProt ID: O43439
• Cytogenetics: 20q11.21-q11.22
• Domains: zf-MYND, TAFH
• Protein Families: Transcription Factors
• Gene Summary: In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq, Jul 2008]