Leucyl tRNA synthetase (LARS) (NM_020117) Human Tagged ORF Clone

CAT#: RG221682

LARS (tGFP-tagged) - Human leucyl-tRNA synthetase (LARS)

Product Images

Circular map for RG221682

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Leucyl tRNA synthetase
• Synonyms: hr025Cl; HSPC192; ILFS1; LARS; LEURS; LEUS; LFIS; LRS; PIG44; RNTLS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221682 representing NM_020117
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAAGAAAAGGAACAGCCAAAGTGGACTTTTTGAAGAAGATTGAGAAAGAAATCCAACAGAAAT
  GGGATACTGAGAGAGTGTTTGAGGTCAATGCATCTAATTTAGAGAAACAGACCAGCAAGGGCAAGTATTT
  TGTAACCTTCCCATATCCATATATGAATGGACGCCTTCATTTGGGACACACGTTTTCTTTATCCAAATGT
  GAGTTTGCTGTAGGGTACCAGCGATTGAAAGGAAAATGTTGTCTGTTTCCCTTTGGCCTGCACTGTACTG
  GAATGCCTATTAAGGCATGTGCTGATAAGTTGAAAAGAGAAATAGAGCTGTATGGTTGCCCCCCTGATTT
  TCCAGATGAAGAAGAGGAAGAGGAAGAAACCAGTGTTAAAACAGAAGATATAATAATTAAGGATAAAGCT
  AAAGGAAAAAAGAGTAAAGCTGCTGCTAAAGCTGGATCTTCTAAATACCAGTGGGGCATTATGAAATCCC
  TTGGCCTGTCTGATGAAGAGATAGTAAAATTTTCTGAAGCAGAACATTGGCTTGATTATTTCCCGCCACT
  GGCTATTCAGGATTTAAAAAGAATGGGTTTGAAGGTAGACTGGCGTCGTTCCTTCATCACCACTGATGTT
  AATCCTTACTATGATTCATTTGTCAGATGGCAATTTTTAACATTAAGAGAAAGAAACAAAATTAAATTTG
  GGAAGCGGTATACAATTTACTCTCCGAAAGATGGACAGCCTTGCATGGATCATGATAGACAAACTGGAGA
  GGGTGTTGGACCTCAGGAATATACTTTACTCAAATTGAAGGCGCTTGAGCCATACCCATCTAAATTAAGT
  GGCCTGAAAGGTAAAAATATTTTCTTGGTGGCTGCTACTCTCAGACCTGAGACCATGTTTGGGCAGACAA
  ATTGTTGGGTTCGTCCTGATATGAAGTACATTGGATTTGAGACGGTGAATGGTGATATATTCATCTGTAC
  CCAAAAAGCAGCCAGGAATATGTCATACCAGGGCTTTACCAAAGACAATGGCGTGGTGCCTGTTGTTAAG
  GAATTAATGGGGGAGGAAATTCTTGGTGCATCACTTTCTGCACCTTTAACATCATACAAGGTGATCTATG
  TTCTCCCAATGCTAACTATTAAGGAGGATAAAGGCACTGGTGTGGTTACAAGTGTTCCTTCCGACTCCCC
  TGATGATATTGCTGCCCTCAGAGACTTGAAGAAAAAGCAAGCCTTACGAGCAAAATATGGAATTAGAGAT
  GACATGGTCTTGCCATTTGAGCCGGTGCCAGTCATTGAAATCCCAGGTTTTGGAAATCTTTCTGCTGTAA
  CCATTTGTGATGAGTTGAAAATTCAGAGCCAGAATGACCGGGAAAAACTTGCAGAAGCAAAGGAGAAGAT
  ATATCTAAAAGGATTTTATGAGGGTATCATGTTGGTGGATGGATTTAAAGGACAGAAGGTTCAAGATGTA
  AAGAAGACTATTCAGAAAAAGATGATTGACGCTGGAGATGCACTTATTTACATGGAACCAGAGAAACAAG
  TGATGTCCAGGTCGTCAGATGAATGTGTTGTGGCTCTGTGTGACCAGTGGTACTTGGATTATGGAGAAGA
  GAATTGGAAGAAACAGACATCTCAGTGCTTGAAGAACCTGGAAACATTCTGTGAGGAGACCAGGAGGAAT
  TTTGAAGCCACCTTAGGTTGGCTACAAGAACATGCTTGCTCAAGAACTTATGGTCTAGGCACTCACCTGC
  CTTGGGATGAGCAGTGGCTGATTGAATCACTTTCTGACTCCACTATTTACATGGCATTTTACACAGTTGC
  ACACCTATTGCAGGGGGGTAACTTGCATGGACAGGCAGAGTCTCCGCTGGGCATTAGACCGCAACAGATG
  ACCAAGGAAGTTTGGGATTATGTTTTCTTCAAGGAGGCTCCATTTCCTAAGACTCAGATTGCAAAGGAAA
  AATTAGATCAGTTAAAGCAGGAGTTTGAATTCTGGTATCCTGTTGATCTTCGCGTCTCTGGCAAGGATCT
  TGTTCCAAATCATCTTTCATATTACCTTTATAATCATGTGGCTATGTGGCCGGAACAAAGTGATAAATGG
  CCTACAGCTGTGAGAGCAAATGGACATCTCCTCCTGAACTCTGAGAAGATGTCAAAATCCACAGGCAACT
  TCCTCACTTTGACCCAAGCTATTGACAAATTTTCAGCAGATGGAATGCGTTTGGCTCTGGCTGATGCTGG
  TGACACTGTAGAAGATGCCAACTTTGTGGAAGCCATGGCAGATGCAGGTATTCTCCGTCTGTACACCTGG
  GTAGAGTGGGTGAAAGAAATGGTTGCCAACTGGGACAGCCTAAGAAGTGGTCCTGCCAGCACTTTCAATG
  ATAGAGTTTTTGCCAGTGAATTGAATGCAGGAATTATAAAAACAGATCAAAACTATGAAAAGATGATGTT
  TAAAGAAGCTTTGAAAACAGGGTTTTTTGAGTTTCAGGCCGCAAAAGATAAGTACCGTGAATTGGCTGTG
  GAAGGGATGCACAGAGAACTTGTGTTCCGGTTTATTGAAGTTCAGACACTTCTCCTCGCTCCATTCTGTC
  CACATTTGTGTGAGCACATCTGGACACTCCTGGGAAAGCCTGACTCAATTATGAATGCTTCATGGCCTGT
  GGCAGGTCCTGTGGATGAAGTTTTAATACACTCCTCACAGTATCTTATGGAAGTAACACATGACCTTAGA
  CTACGACTCAAGAACTATATGATGCCAGCTAAAGGGAAGAAGACCGACAAACAACCCCTGCAGAAGCCCT
  CACATTGCACCATCTATGTGGCAAAGAACTATCCACCTTGGCAACATACCACCCTGTCTGTTCTACGTAA
  ACACTTTGAGGCCAATAACGGAAAACTGCCTGACAACAAAGTCATTGCTAGTGAACTAGGCAGTATGCCA
  GAACTGAAGAAATACATGAAGAAAGTCATGCCATTTGTTGCCATGATTAAGGAAAATCTGGAGAAGATGG
  GGCCTCGTATTCTGGATTTGCAATTAGAATTTGATGAAAAGGCTGTGCTTATGGAGAATATAGTCTATCT
  GACTAATTCGCTTGAGCTAGAACACATAGAAGTCAAGTTTGCCTCCGAAGCAGAAGATAAAATCAGGGAA
  GACTGCTGTCCTGGGAAACCACTTAATGTTTTTAGAATAGAACCTGGTGTGTCCGTTTCTCTGGTGAATC
  CCCAGCCATCCAATGGCCACTTCTCAACCAAAATTGAAATCAAGCAAGGAGATAACTGTGATTCCATAAT
  CAGGCGTTTAATGAAAATGAATCGAGGAATTAAAGACCTTTCCAAAGTGAAACTGATGAGATTTGATGAT
  CCACTGTTGGGGCCTCGACGAGTTCCTGTCCTGGGAAAGGAGTACACCGAGAAGACCCCCATTTCTGAGC
  ATGCTGTTTTCAATGTGGACCTCATGAGCAAGAAAATTCATCTGACTGAGAATGGGATAAGGGTGGATAT
  TGGCGATACAATAATCTATCTGGTTCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221682 representing NM_020117
  Red=Cloning site Green=Tags(s)
  
  MAERKGTAKVDFLKKIEKEIQQKWDTERVFEVNASNLEKQTSKGKYFVTFPYPYMNGRLHLGHTFSLSKC
  EFAVGYQRLKGKCCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPDEEEEEEETSVKTEDIIIKDKA
  KGKKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDV
  NPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKALEPYPSKLS
  GLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVK
  ELMGEEILGASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRD
  DMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDV
  KKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEETRRN
  FEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLHGQAESPLGIRPQQM
  TKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKW
  PTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTW
  VEWVKEMVANWDSLRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAV
  EGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVDEVLIHSSQYLMEVTHDLR
  LRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMP
  ELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIRE
  DCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIKQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDD
  PLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020117
• ORF Size: 3528 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020117.8, NP_064502.8
• RefSeq Size: 4248 bp
• RefSeq ORF: 3531 bp
• Locus ID: 51520
• UniProt ID: Q9P2J5
• Cytogenetics: 5q32
• Domains: tRNA-synt_1
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• Gene Summary: This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. A mutation in this gene was found in affected individuals with infantile liver failure syndrome 1. Alternatively spliced transcript variants of this gene have been observed. [provided by RefSeq, Dec 2015]