Leucyl tRNA synthetase (LARS) Rabbit Polyclonal Antibody

CAT#: TA377959

LARS Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using LARS antibody (TA377959) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

• 

  Immunofluorescence analysis of L929 cells using LARS Rabbit pAb (TA377959) at dilution of 1:100. Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of U2OS cells using LARS Rabbit pAb (TA377959) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human LARS (NP_064502.9).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 55kDa/128kDa/134kDa
• Gene Name: leucyl-tRNA synthetase
• Database Link:
  Entrez Gene 51520 Human
  UniProt ID Q9P2J5
• Background: This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. A mutation in this gene was found in affected individuals with infantile liver failure syndrome 1. Alternatively spliced transcript variants of this gene have been observed.
• Synonyms: FLJ10595; FLJ21788; hr025Cl; HSPC192; KIAA1352; LARS1; LEURS; LEUS; LRS; PIG44; RNTLS