DCP2 (NM_152624) Human Tagged ORF Clone

CAT#: RG221165

DCP2 (tGFP-tagged) - Human DCP2 decapping enzyme homolog (S. cerevisiae) (DCP2)

Product Images

Circular map for RG221165

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DCP2
• Synonyms: NUDT20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221165 representing NM_152624
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCAAACGGGTGGAGATTCCCGGCAGCGTCCTGGACGATCTCTGCAGCCGATTTATTTTGCATA
  TTCCCAGCGAGGAAAGAGACAATGCAATCCGAGTGTGTTTTCAGATTGAACTTGCCCATTGGTTTTACTT
  GGATTTCTACATGCAGAACACACCAGGATTACCTCAGTGTGGGATAAGAGACTTTGCTAAAGCTGTCTTC
  AGTCATTGTCCGTTTTTGCTGCCTCAAGGTGAAGATGTGGAAAAAGTTTTGGATGAATGGAAGGAATATA
  AAATGGGAGTACCAACATATGGTGCAATTATTCTTGATGAGACACTTGAAAATGTACTACTAGTTCAGGG
  GTACCTAGCAAAATCAGGCTGGGGATTTCCAAAAGGAAAAGTAAATAAAGAAGAAGCTCCTCATGATTGT
  GCTGCTAGAGAGGTCTTTGAAGAAACTGGTTTTGATATCAAAGACTATATTTGTAAGGATGATTACATTG
  AACTTCGAATCAATGACCAGCTTGCTCGTTTGTACATCATTCCAGGAATTCCAAAAGACACAAAATTTAA
  CCCAAAAACTAGAAGAGAAATTCGGAACATTGAGTGGTTCTCTATTGAGAAATTGCCTTGTCATAGAAAT
  GATATGACCCCCAAATCCAAACTTGGTTTGGCACCTAACAAATTTTTTATGGCCATTCCCTTTATCAGAC
  CATTAAGGGACTGGCTTTCTCGAAGATTTGGCGATTCCTCAGACAGTGACAATGGATTTTCCTCAACTGG
  TAGCACGCCGGCTAAACCCACTGTGGAAAAATTGAGTCGAACCAAATTCCGCCACAGTCAGCAGTTATTT
  CCTGACGGTTCTCCTGGTGACCAGTGGGTAAAGCACAGGCAACCACTGCAGCAAAAGCCATATAATAATC
  ATTCTGAAATGTCTGACCTTTTAAAAGGAAAGAATCAAAGTATGAGGGGAAATGGCAGAAAACAGTATCA
  AGATTCACCTAATCAAAAGAAAAGAACAAATGGGCTTCAGCCAGCAAAGCAGCAGAATTCTTTGATGAAG
  TGTGAAAAGAAACTTCATCCACGGAAACTTCAGGATAATTTTGAAACAGATGCTGTATATGACTTGCCTA
  GCTCCAGTGAAGACCAGTTGCTAGAACATGCTGAGGGACAGCCCGTGGCATGTAATGGACATTGCAAGTT
  CCCCTTTTCATCCAGAGCCTTTTTGAGTTTCAAGTTTGACCATAATGCTATAATGAAAATCTTGGACCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152624
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152624.6
• RefSeq Size: 8947 bp
• RefSeq ORF: 1263 bp
• Locus ID: 167227
• UniProt ID: Q8IU60
• Cytogenetics: 5q22.2
• Domains: NUDIX, DCP2
• Protein Pathways: RNA degradation
• Gene Summary: The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]