DCP2 (NM_152624) Human Untagged Clone

CAT#: SC100774

DCP2 (untagged)-Human DCP2 decapping enzyme homolog (S. cerevisiae) (DCP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCP2
• Synonyms: NUDT20
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_152624, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGACCAAACGGGTGGAGATTCCCGGCAGCGTCCTGGACGATCTCTGCAGCCGATTTATTTTGCATA
  TTCCCAGCGAGGAAAGAGACAATGCAATCCGAGTGTGTTTTCAGATTGAACTTGCCCATTGGTTTTACTT
  GGATTTCTACATGCAGAACACACCAGGATTACCTCAGTGTGGGATAAGAGACTTTGCTAAAGCTGTCTTC
  AGTCATTGTCCGTTTTTGCTGCCTCAAGGTGAAGATGTGGAAAAAGTTTTGGATGAATGGAAGGAATATA
  AAATGGGAGTACCAACATATGGTGCAATTATTCTTGATGAGACACTTGAAAATGTACTACTAGTTCAGGG
  GTACCTAGCAAAATCAGGCTGGGGATTTCCAAAAGGAAAAGTAAATAAAGAAGAAGCTCCTCATGATTGT
  GCTGCTAGAGAGGTCTTTGAAGAAACTGGTTTTGATATCAAAGACTATATTTGTAAGGATGATTACATTG
  AACTTCGAATCAATGACCAGCTTGCTCGTTTGTACATCATTCCAGGAATTCCAAAAGACACAAAATTTAA
  CCCAAAAACTAGAAGAGAAATTCGGAACATTGAGTGGTTCTCTATTGAGAAATTGCCTTGTCATAGAAAT
  GATATGACCCCCAAATCCAAACTTGGTTTGGCACCTAACAAATTTTTTATGGCCATTCCCTTTATCAGAC
  CATTAAGGGACTGGCTTTCTCGAAGATTTGGCGATTCCTCAGACAGTGACAATGGATTTTCCTCAACTGG
  TAGCACGCCGGCTAAACCCACTGTGGAAAAATTGAGTCGAACCAAATTCCGCCACAGTCAGCAGTTATTT
  CCTGACGGTTCTCCTGGTGACCAGTGGGTAAAGCACAGGCAACCACTGCAGCAAAAGCCATATAATAATC
  ATTCTGAAATGTCTGACCTTTTAAAAGGAAAGAATCAAAGTATGAGGGGAAATGGCAGAAAACAGTATCA
  AGATTCACCTAATCAAAAGAAAAGAACAAATGGGCTTCAGCCAGCAAAGCAGCAGAATTCTTTGATGAAG
  TGTGAAAAGAAACTTCATCCACGGAAACTTCAGGATAATTTTGAAACAGATGCTGTATATGACTTGCCTA
  GCTCCAGTGAAGACCAGTTGCTAGAACATGCTGAGGGACAGCCCGTGGCATGTAATGGACATTGCAAGTT
  CCCCTTTTCATCCAGAGCCTTTTTGAGTTTCAAGTTTGACCATAATGCTATAATGAAAATCTTGGACCTT
  TGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152624 unedited
  GGGTGTCAACTTTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGTCAGTC
  CCCGGCCGCGCGGAGCCGGGATGCACTGTTCCTGCTGTGGGTCCTCATCATGGAGACCAA
  ACGGGTGGAGATTCCCGGCAGCGTCCTGGACGATCTCTGCAGCCGATTTATTTTGCATAT
  TCCCAGCGAGGAAAGAGACAATGCAATCCGAGTGTGTTTTCAGATTGAACTTGCCCATTG
  GTTTTACTTGGATTTCTACATGCAGAACACACCAGGATTACCTCAGTGTGGGATAAGAGA
  CTTTGCTAAAGCTGTCTTCAGTCATTGTCCGTTTTTGCTGCCTCAAGGTGAAGATGTGGA
  AAAAGTTTTGGATGAATGGAAGGAATATAAAATGGGAGTACCAACATATGGTGCAATTAT
  TCTTGATGAGACACTTGAAAATGTACTACTGGTTCAGGGGTACCTAGCAAAATCAGGCTG
  GGGATTTCCAAAAGGAAAAGTAAATAAAGAAGAAGCTCCTCATGATTGTGCTGCTAGAGA
  GGTCTTTGAAGAAACTGGTTTTGATATCAAAGACTATATTTGTAAGGATGATTACATTGA
  ACTTCGAATCAATGACCAGCTTTGCTCGTTTGTACATCATTCCAGGAATTCCAAAAGACA
  CAAAATTTAACCCAAAAACTAGAAGAGAAATTCGGAACATTGAGTGGTTCTCTATTGAGA
  AATTGCCTTGTCATAGAAATGATATGACCCCCAAATCCAAACTTGGTTTGGCACCTAACA
  AATTTTTTATGGGCCATTCCCTTTATCAGACCCATTAAGGACTGGCTTTCTCGAAGATTG
  GCGATTCCTCAGAAGTGACAATGGATTTNCTCACTGGTAGCACGCCGCTAAACCACTGTG
  GAANAATGAGTCGACCAAATTCGCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152624 unedited
  GTTACAGCTATGNNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTGGT
  AGTGAAATGTTTTTAATGCACACATGCACTCAGACCTTAACTTTTCTATAGGATACAATA
  TGGTGACTTTTATAGAAGAATCTAATTCCTATAGTAGGAGGCTGTTATTTACTCAGATTT
  GAATTCCTTTTATTTGACATTACATACAAATTACTAAAGCCTTAATAAGGAAAAGTACCA
  ATTCAATCTTACAGCATAAAATTACTTTCATTATATGTCTTCATTAGCTATACTTACTAA
  TTGATATTTAATAGAAAAATAACGATATTCAAAGCTCAGAACAGCAAGTAGTAACCACAA
  TTCCTTTGACAGCACTGGGAGACTTTCCTTTATACATGAATACAAAAATCCAACTTCATT
  TTCCAACATCTTTAATCAAATTTCACTCACACTATATGAGTTCTATTAGGTTCTCAGGCC
  TCTCCAAATGCTCATGTCTCAGGAGGGTATCACTATTTGAAATCCTAAACCATTCATTTG
  CTACTTCTTAACAAAAATTTTTGAAAAGCATGTAAACCACACCCTTATCTTTTGGATTAC
  AATGGTTAGGTACAAAAACAATTCATTTAGCATCTTATAAATCATTAAAGGTATACATAA
  AAGCACCTACAAATTTTTTTAAAAATAAAAGATTCACAGTGCACTGGCACTTGGAACATA
  TNAAGTTGGAACCGTACATTTAAAAAGTGAATTCCAAGACCTGTATCTCTCTCAAGACCT
  TAAACCTTATGAAGCAAACCAATTCCTTTTCTAATTTACATCTTAAAATGCGCCCTCCCT
  AAAAGTTAATGATTGGCATTTCGCCAAACCCCTTTAAACCTGGGTTCCCAAACCACACCA
  AT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152624
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152624.4, NP_689837.2
• RefSeq Size: 10198 bp
• RefSeq ORF: 1263 bp
• Locus ID: 167227
• UniProt ID: Q8IU60
• Cytogenetics: 5q22.2
• Domains: NUDIX, DCP2
• Protein Pathways: RNA degradation
• Gene Summary: The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.