MYBPC2 (NM_004533) Human Tagged ORF Clone

CAT#: RG220058

MYBPC2 (tGFP-tagged) - Human myosin binding protein C, fast type (MYBPC2)

Product Images

Circular map for RG220058

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MYBPC2
• Synonyms: fsMyBP-C; MYBPC; MYBPCF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220058 representing NM_004533
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAGGCAAAACCAGCGGCCAAAAAGGCCCCCAAAGGCAAAGATGCCCCCAAAGGAGCCCCCAAGG
  AGGCTCCCCCTAAGGAGGCTCCTGCAGAGGCCCCCAAAGAAGCCCCACCCGAGGACCAGTCCCCGACTGC
  AGAGGAGCCCACCGGCGTTTTCCTGAAGAAGCCGGACTCCGTCTCAGTGGAGACTGGGAAGGACGCAGTG
  GTCGTGGCCAAGGTGAACGGGAAGGAGCTCCCAGACAAACCGACCATCAAGTGGTTCAAGGGGAAGTGGC
  TGGAGCTGGGCAGCAAGAGTGGCGCCCGCTTCTCCTTCAAGGAGTCCCACAACTCCGCCAGCAATGTGTA
  CACCGTGGAGCTGCACATTGGGAAGGTGGTACTGGGGGACCGTGGGTATTACCGCCTCGAGGTCAAAGCC
  AAGGACACCTGTGACAGCTGTGGCTTCAACATCGATGTGGAGGCACCCCGTCAGGATGCCTCTGGGCAGA
  GTCTAGAAAGCTTCAAGCGTACGAGTGAAAAGAAGTCGGATACTGCAGGTGAGCTGGATTTCAGTGGCCT
  GTTGAAGAAGAGGGAGGTGGTGGAGGAGGAGAAGAAGAAGAAAAAGAAAGATGACGATGACCTAGGCATC
  CCCCCGGAGATTTGGGAGCTCCTGAAAGGGGCAAAGAAGAGCGAGTACGAGAAAATCGCCTTCCAGTATG
  GCATCACCGACCTCCGGGGCATGCTGAAGCGGCTGAAAAAGGCTAAGGTCGAGGTCAAGAAGAGTGCAGC
  ATTCACAAAGAAGCTGGATCCAGCCTACCAAGTGGACAGAGGCAACAAGATCAAGTTGATGGTAGAGATC
  AGCGACCCAGACCTGACCCTCAAGTGGTTCAAGAACGGCCAGGAGATCAAACCAAGCAGCAAGTACGTGT
  TTGAGAACGTTGGTAAGAAGCGAATTCTTACCATCAACAAGTGCACGCTGGCGGATGACGCTGCCTATGA
  AGTAGCTGTCAAGGATGAGAAGTGTTTCACCGAGCTCTTCGTCAAAGAACCTCCAGTCCTAATTGTCACA
  CCTCTTGAGGACCAGCAGGTGTTTGTGGGTGACCGGGTGGAAATGGCAGTGGAGGTGTCAGAAGAGGGTG
  CCCAGGTGATGTGGATGAAAGATGGTGTGGAACTGACTCGGGAGGATTCCTTCAAGGCCCGGTACCGCTT
  CAAGAAGGACGGGAAGCGCCACATCCTCATCTTCTCAGACGTGGTCCAGGAGGACAGGGGTCGCTATCAG
  GTCATAACCAATGGCGGCCAGTGTGAGGCCGAGCTGATTGTGGAAGAGAAACAGCTGGAGGTCCTGCAGG
  ACATCGCGGATCTGACGGTGAAGGCCTCAGAACAAGCTGTGTTCAAGTGCGAGGTGTCTGATGAGAAAGT
  GACGGGCAAGTGGTATAAGAATGGGGTCGAGGTGCGGCCCAGCAAGAGGATCACCATTTCCCATGTAGGC
  AGGTTCCACAAGCTGGTGATCGATGACGTCCGCCCCGAGGATGAGGGAGACTACACGTTTGTGCCTGACG
  GCTACGCCCTGTCGCTCTCGGCCAAGCTCAACTTCCTGGAAATCAAGGTGGAGTACGTTCCCAAGCAAGA
  GCCACCAAAGATCCACTTGGATTGCTCGGGGAAGACCTCAGAGAATGCGATTGTGGTTGTGGCTGGAAAC
  AAGCTGAGGCTTGACGTGTCCATCACAGGGGAGCCCCCTCCCGTCGCTACCTGGCTGAAGGGAGATGAGG
  TATTCACGACCACCGAGGGCAGGACCCGCATCGAGAAGCGGGTGGACTGCAGCAGCTTTGTGATTGAGAG
  TGCGCAGCGGGAAGACGAGGGCCGCTACACCATCAAGGTCACCAACCCCGTCGGCGAGGACGTGGCTTCC
  ATCTTCCTGCAAGTTGTAGATGTCCCAGACCCCCCGGAGGCTGTGCGCATCACCTCGGTTGGAGAGGATT
  GGGCCATCCTTGTCTGGGAGCCACCAATGTACGATGGGGGGAAGCCAGTCACCGGGTACCTCGTAGAGCG
  GAAGAAGAAGGGCTCTCAGCGCTGGATGAAGCTGAACTTTGAGGTCTTCACAGAGACCACCTATGAGTCC
  ACCAAGATGATCGAGGGCATCCTCTATGAGATGCGTGTCTTCGCCGTCAATGCTATAGGGGTCTCCCAGC
  CCAGCATGAACACCAAGCCTTTTATGCCTATTGCACCCACGAGTGAACCCCTGCACCTGATAGTGGAGGA
  TGTGACAGACACCACCACCACACTCAAGTGGAGGCCTCCGAACAGGATCGGGGCAGGTGGCATCGATGGG
  TACCTGGTGGAGTACTGCCTGGAAGGCTCCGAGGAATGGGTCCCTGCCAACACCGAGCCCGTGGAGCGCT
  GTGGCTTCACCGTCAAGAATCTCCCGACCGGAGCCAGAATCCTCTTCCGAGTAGTTGGGGTCAACATCGC
  GGGGCGCACGGAGCCGGCCACCCTGGCCCAGCCGGTCACCATCAGGGAGATTGCGGAGCCACCCAAGATC
  CGGCTTCCCCGCCATCTCCGCCAGACCTACATCCGCAAAGTGGGCGAGCAGCTCAACCTTGTCGTCCCCT
  TCCAGGGAAAGCCCCGGCCCCAGGTGGTGTGGACCAAGGGCGGGGCCCCGCTGGACACCTCCCGCGTGCA
  CGTGCGGACCAGCGACTTCGACACCGTGTTCTTCGTGCGCCAGGCGGCCCGCTCCGACTCCGGGGAGTAC
  GAGCTGAGCGTGCAGATCGAGAACATGAAGGACACCGCCACCATCCGCATCCGCGTTGTGGAAAAGGCTG
  GGCCCCCCATAAACGTGATGGTGAAGGAGGTGTGGGGCACGAACGCGCTGGTGGAGTGGCAGGCCCCCAA
  AGATGATGGGAACAGTGAGATCATGGGGTATTTCGTCCAGAAAGCAGACAAAAAAACCATGGAGTGGTTC
  AACGTCTATGAACGTAACAGGCACACTAGCTGTACTGTGTCCGACCTTATCGTGGGCAATGAATACTATT
  TCCGAGTTTACACCGAGAACATCTGTGGGCTCAGTGACTCACCTGGTGTCTCCAAGAACACGGCCCGCAT
  CCTCAAGACAGGAATCACCTTCAAACCGTTCGAGTATAAGGAGCATGACTTCCGGATGGCTCCCAAGTTC
  CTGACACCTCTCATAGACCGCGTGGTCGTGGCTGGGTACTCGGCAGCCCTCAACTGTGCTGTCAGAGGCC
  ACCCGAAGCCGAAGGTGGTCTGGATGAAGAACAAGATGGAAATCCGTGAAGATCCCAAGTTCCTGATAAC
  CAATTACCAAGGAGTCCTGACGCTGAACATCCGTCGCCCCTCGCCCTTCGACGCTGGGACTTACACCTGC
  CGGGCCGTCAACGAGCTGGGCGAGGCGCTGGCTGAGTGCAAGCTGGAGGTCCGAGTGCCGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220058 representing NM_004533
  Red=Cloning site Green=Tags(s)
  
  MPEAKPAAKKAPKGKDAPKGAPKEAPPKEAPAEAPKEAPPEDQSPTAEEPTGVFLKKPDSVSVETGKDAV
  VVAKVNGKELPDKPTIKWFKGKWLELGSKSGARFSFKESHNSASNVYTVELHIGKVVLGDRGYYRLEVKA
  KDTCDSCGFNIDVEAPRQDASGQSLESFKRTSEKKSDTAGELDFSGLLKKREVVEEEKKKKKKDDDDLGI
  PPEIWELLKGAKKSEYEKIAFQYGITDLRGMLKRLKKAKVEVKKSAAFTKKLDPAYQVDRGNKIKLMVEI
  SDPDLTLKWFKNGQEIKPSSKYVFENVGKKRILTINKCTLADDAAYEVAVKDEKCFTELFVKEPPVLIVT
  PLEDQQVFVGDRVEMAVEVSEEGAQVMWMKDGVELTREDSFKARYRFKKDGKRHILIFSDVVQEDRGRYQ
  VITNGGQCEAELIVEEKQLEVLQDIADLTVKASEQAVFKCEVSDEKVTGKWYKNGVEVRPSKRITISHVG
  RFHKLVIDDVRPEDEGDYTFVPDGYALSLSAKLNFLEIKVEYVPKQEPPKIHLDCSGKTSENAIVVVAGN
  KLRLDVSITGEPPPVATWLKGDEVFTTTEGRTRIEKRVDCSSFVIESAQREDEGRYTIKVTNPVGEDVAS
  IFLQVVDVPDPPEAVRITSVGEDWAILVWEPPMYDGGKPVTGYLVERKKKGSQRWMKLNFEVFTETTYES
  TKMIEGILYEMRVFAVNAIGVSQPSMNTKPFMPIAPTSEPLHLIVEDVTDTTTTLKWRPPNRIGAGGIDG
  YLVEYCLEGSEEWVPANTEPVERCGFTVKNLPTGARILFRVVGVNIAGRTEPATLAQPVTIREIAEPPKI
  RLPRHLRQTYIRKVGEQLNLVVPFQGKPRPQVVWTKGGAPLDTSRVHVRTSDFDTVFFVRQAARSDSGEY
  ELSVQIENMKDTATIRIRVVEKAGPPINVMVKEVWGTNALVEWQAPKDDGNSEIMGYFVQKADKKTMEWF
  NVYERNRHTSCTVSDLIVGNEYYFRVYTENICGLSDSPGVSKNTARILKTGITFKPFEYKEHDFRMAPKF
  LTPLIDRVVVAGYSAALNCAVRGHPKPKVVWMKNKMEIREDPKFLITNYQGVLTLNIRRPSPFDAGTYTC
  RAVNELGEALAECKLEVRVPQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004533
• ORF Size: 3423 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004533.2, NP_004524.2
• RefSeq Size: 3572 bp
• RefSeq ORF: 3426 bp
• Locus ID: 4606
• UniProt ID: Q14324
• Cytogenetics: 19q13.33
• Domains: ig, IGc2, IG, FN3
• Gene Summary: This gene encodes a member of the myosin-binding protein C family. This family includes the fast-, slow- and cardiac-type isoforms, each of which is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. The protein encoded by this locus is referred to as the fast-type isoform. Mutations in the related but distinct genes encoding the slow-type and cardiac-type isoforms have been associated with distal arthrogryposis, type 1 and hypertrophic cardiomyopathy, respectively. [provided by RefSeq, Jul 2012]