MYBPC2 (NM_004533) Human Tagged ORF Clone

CAT#: RC220058

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MYBPC2 (Myc-DDK-tagged)-Human myosin binding protein C, fast type (MYBPC2)



  "NM_004533" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 993.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MYBPC2
Synonyms fsMyBP-C; MYBPC; MYBPCF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC220058 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGAGGCAAAACCAGCGGCCAAAAAGGCCCCCAAAGGCAAAGATGCCCCCAAAGGAGCCCCCAAGG
AGGCTCCCCCTAAGGAGGCTCCTGCAGAGGCCCCCAAAGAAGCCCCACCCGAGGACCAGTCCCCGACTGC
AGAGGAGCCCACCGGCGTTTTCCTGAAGAAGCCGGACTCCGTCTCAGTGGAGACTGGGAAGGACGCAGTG
GTCGTGGCCAAGGTGAACGGGAAGGAGCTCCCAGACAAACCGACCATCAAGTGGTTCAAGGGGAAGTGGC
TGGAGCTGGGCAGCAAGAGTGGCGCCCGCTTCTCCTTCAAGGAGTCCCACAACTCCGCCAGCAATGTGTA
CACCGTGGAGCTGCACATTGGGAAGGTGGTACTGGGGGACCGTGGGTATTACCGCCTCGAGGTCAAAGCC
AAGGACACCTGTGACAGCTGTGGCTTCAACATCGATGTGGAGGCACCCCGTCAGGATGCCTCTGGGCAGA
GTCTAGAAAGCTTCAAGCGTACGAGTGAAAAGAAGTCGGATACTGCAGGTGAGCTGGATTTCAGTGGCCT
GTTGAAGAAGAGGGAGGTGGTGGAGGAGGAGAAGAAGAAGAAAAAGAAAGATGACGATGACCTAGGCATC
CCCCCGGAGATTTGGGAGCTCCTGAAAGGGGCAAAGAAGAGCGAGTACGAGAAAATCGCCTTCCAGTATG
GCATCACCGACCTCCGGGGCATGCTGAAGCGGCTGAAAAAGGCTAAGGTCGAGGTCAAGAAGAGTGCAGC
ATTCACAAAGAAGCTGGATCCAGCCTACCAAGTGGACAGAGGCAACAAGATCAAGTTGATGGTAGAGATC
AGCGACCCAGACCTGACCCTCAAGTGGTTCAAGAACGGCCAGGAGATCAAACCAAGCAGCAAGTACGTGT
TTGAGAACGTTGGTAAGAAGCGAATTCTTACCATCAACAAGTGCACGCTGGCGGATGACGCTGCCTATGA
AGTAGCTGTCAAGGATGAGAAGTGTTTCACCGAGCTCTTCGTCAAAGAACCTCCAGTCCTAATTGTCACA
CCTCTTGAGGACCAGCAGGTGTTTGTGGGTGACCGGGTGGAAATGGCAGTGGAGGTGTCAGAAGAGGGTG
CCCAGGTGATGTGGATGAAAGATGGTGTGGAACTGACTCGGGAGGATTCCTTCAAGGCCCGGTACCGCTT
CAAGAAGGACGGGAAGCGCCACATCCTCATCTTCTCAGACGTGGTCCAGGAGGACAGGGGTCGCTATCAG
GTCATAACCAATGGCGGCCAGTGTGAGGCCGAGCTGATTGTGGAAGAGAAACAGCTGGAGGTCCTGCAGG
ACATCGCGGATCTGACGGTGAAGGCCTCAGAACAAGCTGTGTTCAAGTGCGAGGTGTCTGATGAGAAAGT
GACGGGCAAGTGGTATAAGAATGGGGTCGAGGTGCGGCCCAGCAAGAGGATCACCATTTCCCATGTAGGC
AGGTTCCACAAGCTGGTGATCGATGACGTCCGCCCCGAGGATGAGGGAGACTACACGTTTGTGCCTGACG
GCTACGCCCTGTCGCTCTCGGCCAAGCTCAACTTCCTGGAAATCAAGGTGGAGTACGTTCCCAAGCAAGA
GCCACCAAAGATCCACTTGGATTGCTCGGGGAAGACCTCAGAGAATGCGATTGTGGTTGTGGCTGGAAAC
AAGCTGAGGCTTGACGTGTCCATCACAGGGGAGCCCCCTCCCGTCGCTACCTGGCTGAAGGGAGATGAGG
TATTCACGACCACCGAGGGCAGGACCCGCATCGAGAAGCGGGTGGACTGCAGCAGCTTTGTGATTGAGAG
TGCGCAGCGGGAAGACGAGGGCCGCTACACCATCAAGGTCACCAACCCCGTCGGCGAGGACGTGGCTTCC
ATCTTCCTGCAAGTTGTAGATGTCCCAGACCCCCCGGAGGCTGTGCGCATCACCTCGGTTGGAGAGGATT
GGGCCATCCTTGTCTGGGAGCCACCAATGTACGATGGGGGGAAGCCAGTCACCGGGTACCTCGTAGAGCG
GAAGAAGAAGGGCTCTCAGCGCTGGATGAAGCTGAACTTTGAGGTCTTCACAGAGACCACCTATGAGTCC
ACCAAGATGATCGAGGGCATCCTCTATGAGATGCGTGTCTTCGCCGTCAATGCTATAGGGGTCTCCCAGC
CCAGCATGAACACCAAGCCTTTTATGCCTATTGCACCCACGAGTGAACCCCTGCACCTGATAGTGGAGGA
TGTGACAGACACCACCACCACACTCAAGTGGAGGCCTCCGAACAGGATCGGGGCAGGTGGCATCGATGGG
TACCTGGTGGAGTACTGCCTGGAAGGCTCCGAGGAATGGGTCCCTGCCAACACCGAGCCCGTGGAGCGCT
GTGGCTTCACCGTCAAGAATCTCCCGACCGGAGCCAGAATCCTCTTCCGAGTAGTTGGGGTCAACATCGC
GGGGCGCAGCGAGCCGGCCACCCTGGCCCAGCCGGTCACCATCAGGGAGATTGCGGAGCCACCCAAGATC
CGGCTTCCCCGCCATCTCCGCCAGACCTACATCCGCAAAGTGGGCGAGCAGCTCAACCTTGTCGTCCCCT
TCCAGGGAAAGCCCCGGCCCCAGGTGGTGTGGACCAAGGGCGGGGCCCCGCTGGACACCTCCCGCGTGCA
CGTGCGGACCAGCGACTTCGACACCGTGTTCTTCGTGCGCCAGGCGGCCCGCTCCGACTCCGGGGAGTAC
GAGCTGAGCGTGCAGATCGAGAACATGAAGGACACCGCCACCATCCGCATCCGCGTTGTGGAAAAGGCTG
GGCCCCCCATAAACGTGATGGTGAAGGAGGTGTGGGGCACGAACGCGCTGGTGGAGTGGCAGGCCCCCAA
AGATGATGGGAACAGTGAGATCATGGGGTATTTCGTCCAGAAAGCAGACAAAAAAACCATGGAGTGGTTC
AACGTCTATGAACGTAACAGGCACACTAGCTGTACTGTGTCCGACCTTATCGTGGGCAATGAATACTATT
TCCGAGTTTACACCGAGAACATCTGTGGGCTCAGTGACTCACCTGGTGTCTCCAAGAACACGGCCCGCAT
CCTCAAGACAGGAATCACCTTCAAACCGTTCGAGTATAAGGAGCATGACTTCCGGATGGCTCCCAAGTTC
CTGACACCTCTCATAGACCGCGTGGTCGTGGCTGGGTACTCGGCAGCCCTCAACTGCGCTGTCAGAGGCC
ACCCGAAGCCGAAGGTGGTCTGGATGAAGAACAAGATGGAAATCCGTGAAGATCCCAAGTTCCTGATAAC
CAATTACCAAGGAGTCCTGACGCTGAACATCCGTCGCCCCTCGCCCTTCGACGCTGGGACTTACACCTGC
CGGGCCGTCAACGAGCTGGGCGAGGCGCTGGCTGAGTGCAAGCTGGAGGTCCGAGTGCCGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC220058 protein sequence
Red=Cloning site Green=Tags(s)

MPEAKPAAKKAPKGKDAPKGAPKEAPPKEAPAEAPKEAPPEDQSPTAEEPTGVFLKKPDSVSVETGKDAV
VVAKVNGKELPDKPTIKWFKGKWLELGSKSGARFSFKESHNSASNVYTVELHIGKVVLGDRGYYRLEVKA
KDTCDSCGFNIDVEAPRQDASGQSLESFKRTSEKKSDTAGELDFSGLLKKREVVEEEKKKKKKDDDDLGI
PPEIWELLKGAKKSEYEKIAFQYGITDLRGMLKRLKKAKVEVKKSAAFTKKLDPAYQVDRGNKIKLMVEI
SDPDLTLKWFKNGQEIKPSSKYVFENVGKKRILTINKCTLADDAAYEVAVKDEKCFTELFVKEPPVLIVT
PLEDQQVFVGDRVEMAVEVSEEGAQVMWMKDGVELTREDSFKARYRFKKDGKRHILIFSDVVQEDRGRYQ
VITNGGQCEAELIVEEKQLEVLQDIADLTVKASEQAVFKCEVSDEKVTGKWYKNGVEVRPSKRITISHVG
RFHKLVIDDVRPEDEGDYTFVPDGYALSLSAKLNFLEIKVEYVPKQEPPKIHLDCSGKTSENAIVVVAGN
KLRLDVSITGEPPPVATWLKGDEVFTTTEGRTRIEKRVDCSSFVIESAQREDEGRYTIKVTNPVGEDVAS
IFLQVVDVPDPPEAVRITSVGEDWAILVWEPPMYDGGKPVTGYLVERKKKGSQRWMKLNFEVFTETTYES
TKMIEGILYEMRVFAVNAIGVSQPSMNTKPFMPIAPTSEPLHLIVEDVTDTTTTLKWRPPNRIGAGGIDG
YLVEYCLEGSEEWVPANTEPVERCGFTVKNLPTGARILFRVVGVNIAGRSEPATLAQPVTIREIAEPPKI
RLPRHLRQTYIRKVGEQLNLVVPFQGKPRPQVVWTKGGAPLDTSRVHVRTSDFDTVFFVRQAARSDSGEY
ELSVQIENMKDTATIRIRVVEKAGPPINVMVKEVWGTNALVEWQAPKDDGNSEIMGYFVQKADKKTMEWF
NVYERNRHTSCTVSDLIVGNEYYFRVYTENICGLSDSPGVSKNTARILKTGITFKPFEYKEHDFRMAPKF
LTPLIDRVVVAGYSAALNCAVRGHPKPKVVWMKNKMEIREDPKFLITNYQGVLTLNIRRPSPFDAGTYTC
RAVNELGEALAECKLEVRVPQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004533
ORF Size 3423 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004533.4
RefSeq Size 3598 bp
RefSeq ORF 3426 bp
Locus ID 4606
UniProt ID Q14324
Cytogenetics 19q13.33
Domains ig, IGc2, IG, FN3
MW 128.1 kDa
Gene Summary This gene encodes a member of the myosin-binding protein C family. This family includes the fast-, slow- and cardiac-type isoforms, each of which is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. The protein encoded by this locus is referred to as the fast-type isoform. Mutations in the related but distinct genes encoding the slow-type and cardiac-type isoforms have been associated with distal arthrogryposis, type 1 and hypertrophic cardiomyopathy, respectively. [provided by RefSeq, Jul 2012]

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