MYBPC2 (NM_004533) Human Tagged ORF Clone

CAT#: RC220058

MYBPC2 (Myc-DDK-tagged)-Human myosin binding protein C, fast type (MYBPC2)

Product Images

Circular map for RC220058

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MYBPC2 (Cat# RC220058, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYBPC2 (Cat# TA811203)(1:2000). Positive lysates LY417926 (100ug) and LC417926 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY417926) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220058 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MYBPC2 protein (Cat# TP320058). The protein was produced from HEK293T cells transfected with MYBPC2 cDNA clone (Cat# RC220058) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MYBPC2
• Synonyms: fsMyBP-C; MYBPC; MYBPCF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220058 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAGGCAAAACCAGCGGCCAAAAAGGCCCCCAAAGGCAAAGATGCCCCCAAAGGAGCCCCCAAGG
  AGGCTCCCCCTAAGGAGGCTCCTGCAGAGGCCCCCAAAGAAGCCCCACCCGAGGACCAGTCCCCGACTGC
  AGAGGAGCCCACCGGCGTTTTCCTGAAGAAGCCGGACTCCGTCTCAGTGGAGACTGGGAAGGACGCAGTG
  GTCGTGGCCAAGGTGAACGGGAAGGAGCTCCCAGACAAACCGACCATCAAGTGGTTCAAGGGGAAGTGGC
  TGGAGCTGGGCAGCAAGAGTGGCGCCCGCTTCTCCTTCAAGGAGTCCCACAACTCCGCCAGCAATGTGTA
  CACCGTGGAGCTGCACATTGGGAAGGTGGTACTGGGGGACCGTGGGTATTACCGCCTCGAGGTCAAAGCC
  AAGGACACCTGTGACAGCTGTGGCTTCAACATCGATGTGGAGGCACCCCGTCAGGATGCCTCTGGGCAGA
  GTCTAGAAAGCTTCAAGCGTACGAGTGAAAAGAAGTCGGATACTGCAGGTGAGCTGGATTTCAGTGGCCT
  GTTGAAGAAGAGGGAGGTGGTGGAGGAGGAGAAGAAGAAGAAAAAGAAAGATGACGATGACCTAGGCATC
  CCCCCGGAGATTTGGGAGCTCCTGAAAGGGGCAAAGAAGAGCGAGTACGAGAAAATCGCCTTCCAGTATG
  GCATCACCGACCTCCGGGGCATGCTGAAGCGGCTGAAAAAGGCTAAGGTCGAGGTCAAGAAGAGTGCAGC
  ATTCACAAAGAAGCTGGATCCAGCCTACCAAGTGGACAGAGGCAACAAGATCAAGTTGATGGTAGAGATC
  AGCGACCCAGACCTGACCCTCAAGTGGTTCAAGAACGGCCAGGAGATCAAACCAAGCAGCAAGTACGTGT
  TTGAGAACGTTGGTAAGAAGCGAATTCTTACCATCAACAAGTGCACGCTGGCGGATGACGCTGCCTATGA
  AGTAGCTGTCAAGGATGAGAAGTGTTTCACCGAGCTCTTCGTCAAAGAACCTCCAGTCCTAATTGTCACA
  CCTCTTGAGGACCAGCAGGTGTTTGTGGGTGACCGGGTGGAAATGGCAGTGGAGGTGTCAGAAGAGGGTG
  CCCAGGTGATGTGGATGAAAGATGGTGTGGAACTGACTCGGGAGGATTCCTTCAAGGCCCGGTACCGCTT
  CAAGAAGGACGGGAAGCGCCACATCCTCATCTTCTCAGACGTGGTCCAGGAGGACAGGGGTCGCTATCAG
  GTCATAACCAATGGCGGCCAGTGTGAGGCCGAGCTGATTGTGGAAGAGAAACAGCTGGAGGTCCTGCAGG
  ACATCGCGGATCTGACGGTGAAGGCCTCAGAACAAGCTGTGTTCAAGTGCGAGGTGTCTGATGAGAAAGT
  GACGGGCAAGTGGTATAAGAATGGGGTCGAGGTGCGGCCCAGCAAGAGGATCACCATTTCCCATGTAGGC
  AGGTTCCACAAGCTGGTGATCGATGACGTCCGCCCCGAGGATGAGGGAGACTACACGTTTGTGCCTGACG
  GCTACGCCCTGTCGCTCTCGGCCAAGCTCAACTTCCTGGAAATCAAGGTGGAGTACGTTCCCAAGCAAGA
  GCCACCAAAGATCCACTTGGATTGCTCGGGGAAGACCTCAGAGAATGCGATTGTGGTTGTGGCTGGAAAC
  AAGCTGAGGCTTGACGTGTCCATCACAGGGGAGCCCCCTCCCGTCGCTACCTGGCTGAAGGGAGATGAGG
  TATTCACGACCACCGAGGGCAGGACCCGCATCGAGAAGCGGGTGGACTGCAGCAGCTTTGTGATTGAGAG
  TGCGCAGCGGGAAGACGAGGGCCGCTACACCATCAAGGTCACCAACCCCGTCGGCGAGGACGTGGCTTCC
  ATCTTCCTGCAAGTTGTAGATGTCCCAGACCCCCCGGAGGCTGTGCGCATCACCTCGGTTGGAGAGGATT
  GGGCCATCCTTGTCTGGGAGCCACCAATGTACGATGGGGGGAAGCCAGTCACCGGGTACCTCGTAGAGCG
  GAAGAAGAAGGGCTCTCAGCGCTGGATGAAGCTGAACTTTGAGGTCTTCACAGAGACCACCTATGAGTCC
  ACCAAGATGATCGAGGGCATCCTCTATGAGATGCGTGTCTTCGCCGTCAATGCTATAGGGGTCTCCCAGC
  CCAGCATGAACACCAAGCCTTTTATGCCTATTGCACCCACGAGTGAACCCCTGCACCTGATAGTGGAGGA
  TGTGACAGACACCACCACCACACTCAAGTGGAGGCCTCCGAACAGGATCGGGGCAGGTGGCATCGATGGG
  TACCTGGTGGAGTACTGCCTGGAAGGCTCCGAGGAATGGGTCCCTGCCAACACCGAGCCCGTGGAGCGCT
  GTGGCTTCACCGTCAAGAATCTCCCGACCGGAGCCAGAATCCTCTTCCGAGTAGTTGGGGTCAACATCGC
  GGGGCGCAGCGAGCCGGCCACCCTGGCCCAGCCGGTCACCATCAGGGAGATTGCGGAGCCACCCAAGATC
  CGGCTTCCCCGCCATCTCCGCCAGACCTACATCCGCAAAGTGGGCGAGCAGCTCAACCTTGTCGTCCCCT
  TCCAGGGAAAGCCCCGGCCCCAGGTGGTGTGGACCAAGGGCGGGGCCCCGCTGGACACCTCCCGCGTGCA
  CGTGCGGACCAGCGACTTCGACACCGTGTTCTTCGTGCGCCAGGCGGCCCGCTCCGACTCCGGGGAGTAC
  GAGCTGAGCGTGCAGATCGAGAACATGAAGGACACCGCCACCATCCGCATCCGCGTTGTGGAAAAGGCTG
  GGCCCCCCATAAACGTGATGGTGAAGGAGGTGTGGGGCACGAACGCGCTGGTGGAGTGGCAGGCCCCCAA
  AGATGATGGGAACAGTGAGATCATGGGGTATTTCGTCCAGAAAGCAGACAAAAAAACCATGGAGTGGTTC
  AACGTCTATGAACGTAACAGGCACACTAGCTGTACTGTGTCCGACCTTATCGTGGGCAATGAATACTATT
  TCCGAGTTTACACCGAGAACATCTGTGGGCTCAGTGACTCACCTGGTGTCTCCAAGAACACGGCCCGCAT
  CCTCAAGACAGGAATCACCTTCAAACCGTTCGAGTATAAGGAGCATGACTTCCGGATGGCTCCCAAGTTC
  CTGACACCTCTCATAGACCGCGTGGTCGTGGCTGGGTACTCGGCAGCCCTCAACTGCGCTGTCAGAGGCC
  ACCCGAAGCCGAAGGTGGTCTGGATGAAGAACAAGATGGAAATCCGTGAAGATCCCAAGTTCCTGATAAC
  CAATTACCAAGGAGTCCTGACGCTGAACATCCGTCGCCCCTCGCCCTTCGACGCTGGGACTTACACCTGC
  CGGGCCGTCAACGAGCTGGGCGAGGCGCTGGCTGAGTGCAAGCTGGAGGTCCGAGTGCCGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220058 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPEAKPAAKKAPKGKDAPKGAPKEAPPKEAPAEAPKEAPPEDQSPTAEEPTGVFLKKPDSVSVETGKDAV
  VVAKVNGKELPDKPTIKWFKGKWLELGSKSGARFSFKESHNSASNVYTVELHIGKVVLGDRGYYRLEVKA
  KDTCDSCGFNIDVEAPRQDASGQSLESFKRTSEKKSDTAGELDFSGLLKKREVVEEEKKKKKKDDDDLGI
  PPEIWELLKGAKKSEYEKIAFQYGITDLRGMLKRLKKAKVEVKKSAAFTKKLDPAYQVDRGNKIKLMVEI
  SDPDLTLKWFKNGQEIKPSSKYVFENVGKKRILTINKCTLADDAAYEVAVKDEKCFTELFVKEPPVLIVT
  PLEDQQVFVGDRVEMAVEVSEEGAQVMWMKDGVELTREDSFKARYRFKKDGKRHILIFSDVVQEDRGRYQ
  VITNGGQCEAELIVEEKQLEVLQDIADLTVKASEQAVFKCEVSDEKVTGKWYKNGVEVRPSKRITISHVG
  RFHKLVIDDVRPEDEGDYTFVPDGYALSLSAKLNFLEIKVEYVPKQEPPKIHLDCSGKTSENAIVVVAGN
  KLRLDVSITGEPPPVATWLKGDEVFTTTEGRTRIEKRVDCSSFVIESAQREDEGRYTIKVTNPVGEDVAS
  IFLQVVDVPDPPEAVRITSVGEDWAILVWEPPMYDGGKPVTGYLVERKKKGSQRWMKLNFEVFTETTYES
  TKMIEGILYEMRVFAVNAIGVSQPSMNTKPFMPIAPTSEPLHLIVEDVTDTTTTLKWRPPNRIGAGGIDG
  YLVEYCLEGSEEWVPANTEPVERCGFTVKNLPTGARILFRVVGVNIAGRSEPATLAQPVTIREIAEPPKI
  RLPRHLRQTYIRKVGEQLNLVVPFQGKPRPQVVWTKGGAPLDTSRVHVRTSDFDTVFFVRQAARSDSGEY
  ELSVQIENMKDTATIRIRVVEKAGPPINVMVKEVWGTNALVEWQAPKDDGNSEIMGYFVQKADKKTMEWF
  NVYERNRHTSCTVSDLIVGNEYYFRVYTENICGLSDSPGVSKNTARILKTGITFKPFEYKEHDFRMAPKF
  LTPLIDRVVVAGYSAALNCAVRGHPKPKVVWMKNKMEIREDPKFLITNYQGVLTLNIRRPSPFDAGTYTC
  RAVNELGEALAECKLEVRVPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004533
• ORF Size: 3423 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004533.4
• RefSeq Size: 3598 bp
• RefSeq ORF: 3426 bp
• Locus ID: 4606
• UniProt ID: Q14324
• Cytogenetics: 19q13.33
• Domains: ig, IGc2, IG, FN3
• MW: 128.1 kDa
• Gene Summary: This gene encodes a member of the myosin-binding protein C family. This family includes the fast-, slow- and cardiac-type isoforms, each of which is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. The protein encoded by this locus is referred to as the fast-type isoform. Mutations in the related but distinct genes encoding the slow-type and cardiac-type isoforms have been associated with distal arthrogryposis, type 1 and hypertrophic cardiomyopathy, respectively. [provided by RefSeq, Jul 2012]