GABA A Receptor beta 2 (GABRB2) (NM_021911) Human Tagged ORF Clone

CAT#: RG217467

GABRB2 (tGFP-tagged) - Human gamma-aminobutyric acid (GABA) A receptor, beta 2 (GABRB2), transcript variant 1

Product Images

Circular map for RG217467

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GABA A Receptor beta 2
• Synonyms: DEE92; ICEE2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217467 representing NM_021911
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGAGTCCGGAAAAGGGGCTACTTTGGGATTTGGTCCTTCCCCTTAATAATCGCCGCTGTCTGTG
  CGCAGAGTGTCAATGACCCTAGTAATATGTCGCTGGTTAAAGAGACGGTGGATAGACTCCTGAAAGGCTA
  TGACATTCGTCTGAGACCAGATTTTGGAGGTCCCCCCGTGGCTGTGGGGATGAACATTGACATTGCCAGC
  ATCGATATGGTTTCTGAAGTCAATATGGATTATACCTTGACAATGTACTTTCAACAAGCCTGGAGAGATA
  AGAGGCTGTCCTATAATGTAATACCTTTAAACTTGACTCTGGACAACAGAGTGGCAGACCAGCTCTGGGT
  GCCTGATACCTATTTCCTGAACGATAAGAAGTCATTTGTGCACGGAGTGACTGTTAAGAACCGCATGATT
  CGCCTGCATCCTGATGGCACCGTCCTTTATGGACTCAGAATCACAACCACAGCTGCCTGCATGATGGACC
  TAAGGAGGTACCCACTGGATGAACAAAACTGCACCTTGGAAATTGAGAGCTATGGATACACAACTGATGA
  CATTGAGTTTTACTGGCGTGGCGATGATAATGCAGTAACAGGAGTAACGAAAATTGAACTTCCACAGTTC
  TCTATTGTAGATTACAAACTTATCACCAAGAAGGTTGTTTTTTCCACAGGTTCCTATCCCAGGTTATCCC
  TCAGCTTTAAGCTTAAGAGAAACATTGGCTACTTTATCCTGCAAACATACATGCCTTCCATCCTGATTAC
  CATCCTCTCCTGGGTCTCCTTCTGGATTAATTACGATGCTTCAGCTGCAAGGGTGGCATTAGGAATCACA
  ACTGTCCTCACAATGACCACAATCAACACCCACCTCCGGGAAACTCTCCCTAAAATCCCCTATGTGAAGG
  CCATTGACATGTACCTGATGGGGTGCTTTGTCTTCGTTTTCATGGCCCTTCTGGAATATGCCCTAGTCAA
  CTACATCTTCTTTGGGAGGGGGCCCCAACGCCAAAAGAAAGCAGCTGAGAAGGCTGCCAGTGCCAACAAT
  GAGAAGATGCGCCTGGATGTCAACAAGATTTTTTATAAAGATATTAAACAAAATGGGACCCAATATCGAT
  CCTTGTGGGACCCTACTGGAAACCTCTCCCCAACTAGACGGACTACCAATTACGATTTCTCTCTGTATAC
  GATGGACCCCCATGAGAACATCTTACTGAGCACTCTCGAGATAAAAAATGAAATGGCCACATCTGAGGCT
  GTGATGGGACTTGGAGACCCCAGAAGCACAATGCTAGCCTATGATGCCTCCAGCATCCAGTATCGGAAAG
  CTGGGTTGCCCAGGCATAGTTTTGGCCGAAATGCTCTGGAACGACATGTGGCGCAAAAGAAAAGTCGCCT
  GAGGAGACGCGCCTCCCAACTGAAAATCACCATCCCTGACTTGACTGATGTGAATGCCATAGATCGGTGG
  TCCCGCATATTCTTCCCAGTGGTTTTTTCCTTCTTCAACATCGTCTATTGGCTTTACTATGTGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217467 representing NM_021911
  Red=Cloning site Green=Tags(s)
  
  MWRVRKRGYFGIWSFPLIIAAVCAQSVNDPSNMSLVKETVDRLLKGYDIRLRPDFGGPPVAVGMNIDIAS
  IDMVSEVNMDYTLTMYFQQAWRDKRLSYNVIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMI
  RLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGDDNAVTGVTKIELPQF
  SIVDYKLITKKVVFSTGSYPRLSLSFKLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARVALGIT
  TVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFMALLEYALVNYIFFGRGPQRQKKAAEKAASANN
  EKMRLDVNKIFYKDIKQNGTQYRSLWDPTGNLSPTRRTTNYDFSLYTMDPHENILLSTLEIKNEMATSEA
  VMGLGDPRSTMLAYDASSIQYRKAGLPRHSFGRNALERHVAQKKSRLRRRASQLKITIPDLTDVNAIDRW
  SRIFFPVVFSFFNIVYWLYYVN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021911
• ORF Size: 1536 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021911.1, NP_068711.1
• RefSeq Size: 1974 bp
• RefSeq ORF: 1539 bp
• Locus ID: 2561
• UniProt ID: P47870
• Cytogenetics: 5q34
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes GABA A receptor, beta 2 subunit. It is mapped to chromosome 5q34 in a cluster comprised of genes encoding alpha 1 and gamma 2 subunits of the GABA A receptor. Alternative splicing of this gene generates 2 transcript variants, differing by a 114 bp insertion. [provided by RefSeq, Jul 2008]