HDAC7 (NM_015401) Human Tagged ORF Clone

CAT#: RG215233

HDAC7 (tGFP-tagged) - Human histone deacetylase 7 (HDAC7), transcript variant 1

Product Images

Circular map for RG215233

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HDAC7
• Synonyms: HD7; HD7A; HDAC7A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215233 representing NM_015401
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACAGCCCCGGCGCTGATGGGACCCAGGTGAGCCCGGGTGCCCACTACTGCAGCCCCACTGGCGCAG
  GCTGCCCCAGGCCCTGTGCAGACACACCAGGCCCTCAGCCGCAGCCCATGGACCTGCGGGTGGGCCAGCG
  GCCCCCAGTGGAGCCCCCACCAGAGCCCACATTGCTGGCCCTGCAGCGTCCCCAGCGCCTGCACCACCAC
  CTCTTCCTAGCAGGCCTGCAGCAGCAGCGCTCGGTGGAGCCCATGAGGCTCTCCATGGACACGCCGATGC
  CCGAGTTGCAGGTGGGACCCCAGGAACAAGAGCTGCGGCAGCTTCTCCACAAGGACAAGAGCAAGCGAAG
  TGCTGTAGCCAGCAGCGTGGTCAAGCAGAAGCTAGCGGAGGTGATTCTGAAAAAACAGCAGGCGGCCCTA
  GAAAGAACAGTCCATCCCAACAGCCCCGGCATTCCCTACAGAACCCTGGAGCCCCTGGAGACGGAAGGAG
  CCACCCGCTCCATGCTCAGCAGCTTTTTGCCTCCTGTTCCCAGCCTGCCCAGTGACCCCCCAGAGCACTT
  CCCTCTGCGCAAGACAGTCTCTGAGCCCAACCTGAAGCTGCGCTATAAGCCCAAGAAGTCCCTGGAGCGG
  AGGAAGAATCCACTGCTCCGAAAGGAGAGTGCGCCCCCCAGCCTCCGGCGGCGGCCCGCAGAGACCCTCG
  GAGACTCCTCCCCAAGTAGTAGCAGCACGCCCGCATCAGGGTGCAGCTCCCCCAATGACAGCGAGCACGG
  CCCCAATCCCATCCTGGGCTCGGAGGCGCTCTTGGGCCAGCGGCTGCGGCTGCAGGAGACTTCTGTGGCC
  CCGTTCGCCTTGCCGACAGTGTCCTTGCTGCCCGCAATCACTCTGGGGCTGCCCGCCCCTGCCAGGGCTG
  ACAGTGACCGCAGGACCCATCCGACTCTGGGCCCTCGGGGGCCAATCCTGGGGAGCCCCCACACTCCCCT
  CTTCCTGCCCCATGGCTTGGAGCCCGAGGCTGGGGGCACCTTGCCCTCTCGCCTGCAGCCCATTCTCCTC
  CTGGACCCCTCAGGCTCTCATGCCCCGCTGCTGACTGTGCCCGGGCTTGGGCCCTTGCCCTTCCACTTTG
  CCCAGTCCTTAATGACCACCGAGCGGCTCTCTGGGTCAGGCCTCCACTGGCCACTGAGCCGGACTCGCTC
  AGAGCCCCTGCCCCCCAGTGCCACCGCTCCCCCACCGCCGGGCCCCATGCAGCCCCGCCTGGAGCAGCTC
  AAAACTCACGTCCAGGTGATCAAGAGGTCAGCCAAGCCGAGTGAGAAGCCCCGGCTGCGGCAGATACCCT
  CGGCTGAAGACCTGGAGACAGATGGCGGGGGACCGGGCCAGGTGGTGGACGATGGCCTGGAGCACAGGGA
  GCTGGGCCATGGGCAGCCTGAGGCCAGAGGCCCCGCTCCTCTCCAGCAGCACCCTCAGGTGTTGCTCTGG
  GAACAGCAGCGACTGGCTGGGCGGCTCCCCCGGGGCAGCACCGGGGACACTGTGCTGCTTCCTCTGGCCC
  AGGGTGGGCACCGGCCTCTGTCCCGGGCTCAGTCTTCCCCAGCCGCACCTGCCTCACTGTCAGCCCCAGA
  GCCTGCCAGCCAGGCCCGAGTCCTCTCCAGCTCAGAGACCCCTGCCAGGACCCTGCCCTTCACCACAGGG
  CTGATCTATGACTCGGTCATGCTGAAGCACCAGTGCTCCTGCGGTGACAACAGCAGGCACCCGGAGCACG
  CCGGCCGCATCCAGAGCATCTGGTCCCGGCTGCAGGAGCGGGGGCTCCGGAGCCAGTGTGAGTGTCTCCG
  AGGCCGGAAGGCCTCCCTGGAAGAGCTGCAGTCGGTCCACTCTGAGCGGCACGTGCTCCTCTACGGCACC
  AACCCGCTCAGCCGCCTCAAACTGGACAACGGGAAGCTGGCAGGGCTCCTGGCACAGCGGATGTTTGTGA
  TGCTGCCCTGTGGTGGGGTTGGGGTGGACACTGACACCATCTGGAATGAGCTTCATTCCTCCAATGCAGC
  CCGCTGGGCCGCTGGCAGTGTCACTGACCTCGCCTTCAAAGTGGCTTCTCGTGAGCTAAAGAATGGTTTC
  GCTGTGGTGCGGCCCCCAGGACACCATGCAGATCATTCAACAGCCATGGGCTTCTGCTTCTTCAACTCAG
  TGGCCATCGCCTGCCGGCAGCTGCAACAGCAGAGCAAGGCCAGCAAGATCCTCATTGTAGACTGGGACGT
  GCACCATGGCAACGGCACCCAGCAAACCTTCTACCAAGACCCCAGTGTGCTCTACATCTCCCTGCATCGC
  CATGACGACGGCAACTTCTTCCCGGGGAGTGGGGCTGTGGATGAGGTAGGGGCTGGCAGCGGTGAGGGCT
  TCAATGTCAATGTGGCCTGGGCTGGAGGTCTGGACCCCCCCATGGGGGATCCTGAGTACCTGGCTGCTTT
  CAGGATAGTCGTGATGCCCATCGCCCGAGAGTTCTCTCCAGACCTAGTCCTGGTGTCTGCTGGATTTGAT
  GCTGCTGAGGGTCACCCGGCCCCACTGGGTGGCTACCATGTTTCTGCCAAATGTTTTGGATACATGACGC
  AGCAACTGATGAACCTGGCAGGAGGCGCAGTGGTGCTGGCCTTGGAGGGTGGCCATGACCTCACAGCCAT
  CTGTGACGCCTCTGAGGCCTGTGTGGCTGCTCTTCTGGGTAACAGGGTGGATCCCCTTTCAGAAGAAGGC
  TGGAAACAGAAACCCAACCTCAATGCCATCCGCTCTCTGGAGGCCGTGATCCGGGTGCACAGTAAATACT
  GGGGCTGCATGCAGCGCCTGGCCTCCTGTCCAGACTCCTGGGTGCCTAGAGTGCCAGGGGCTGACAAAGA
  AGAAGTGGAGGCAGTGACCGCACTGGCGTCCCTCTCTGTGGGCATCCTGGCTGAAGATAGGCCCTCGGAG
  CAGCTGGTGGAGGAGGAAGAACCTATGAATCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215233 representing NM_015401
  Red=Cloning site Green=Tags(s)
  
  MHSPGADGTQVSPGAHYCSPTGAGCPRPCADTPGPQPQPMDLRVGQRPPVEPPPEPTLLALQRPQRLHHH
  LFLAGLQQQRSVEPMRLSMDTPMPELQVGPQEQELRQLLHKDKSKRSAVASSVVKQKLAEVILKKQQAAL
  ERTVHPNSPGIPYRTLEPLETEGATRSMLSSFLPPVPSLPSDPPEHFPLRKTVSEPNLKLRYKPKKSLER
  RKNPLLRKESAPPSLRRRPAETLGDSSPSSSSTPASGCSSPNDSEHGPNPILGSEALLGQRLRLQETSVA
  PFALPTVSLLPAITLGLPAPARADSDRRTHPTLGPRGPILGSPHTPLFLPHGLEPEAGGTLPSRLQPILL
  LDPSGSHAPLLTVPGLGPLPFHFAQSLMTTERLSGSGLHWPLSRTRSEPLPPSATAPPPPGPMQPRLEQL
  KTHVQVIKRSAKPSEKPRLRQIPSAEDLETDGGGPGQVVDDGLEHRELGHGQPEARGPAPLQQHPQVLLW
  EQQRLAGRLPRGSTGDTVLLPLAQGGHRPLSRAQSSPAAPASLSAPEPASQARVLSSSETPARTLPFTTG
  LIYDSVMLKHQCSCGDNSRHPEHAGRIQSIWSRLQERGLRSQCECLRGRKASLEELQSVHSERHVLLYGT
  NPLSRLKLDNGKLAGLLAQRMFVMLPCGGVGVDTDTIWNELHSSNAARWAAGSVTDLAFKVASRELKNGF
  AVVRPPGHHADHSTAMGFCFFNSVAIACRQLQQQSKASKILIVDWDVHHGNGTQQTFYQDPSVLYISLHR
  HDDGNFFPGSGAVDEVGAGSGEGFNVNVAWAGGLDPPMGDPEYLAAFRIVVMPIAREFSPDLVLVSAGFD
  AAEGHPAPLGGYHVSAKCFGYMTQQLMNLAGGAVVLALEGGHDLTAICDASEACVAALLGNRVDPLSEEG
  WKQKPNLNAIRSLEAVIRVHSKYWGCMQRLASCPDSWVPRVPGADKEEVEAVTALASLSVGILAEDRPSE
  QLVEEEEPMNL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015401
• ORF Size: 2973 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015401.5
• RefSeq Size: 4301 bp
• RefSeq ORF: 2976 bp
• Locus ID: 51564
• UniProt ID: Q8WUI4
• Cytogenetics: 12q13.11
• Domains: Hist_deacetyl
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]