SRP68 (NM_014230) Human Tagged ORF Clone

CAT#: RG214724

SRP68 (tGFP-tagged) - Human signal recognition particle 68kDa (SRP68)

Product Images

Circular map for RG214724

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SRP68
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214724 representing NM_014230
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGAGAAGCAGGTCCCAGGCGGCGGCGGCGGCGGCGGCAGTGGCGGCGGCGGTGGCAGTGGCG
  GCGGCGGTAGCGGCGGTGGACGTGGTGCCGGAGGGGAAGAAAATAAAGAAAACGAACGCCCTTCGGCCGG
  ATCGAAGGCAAACAAAGAATTTGGGGATAGCCTGAGTTTGGAGATTCTTCAGATTATTAAGGAATCCCAG
  CAGCAGCATGGTTTACGGCATGGAGATTTTCAGAGGTACAGGGGCTACTGTTCCCGTAGACAAAGACGTC
  TTCGAAAAACACTCAACTTCAAGATGGGTAACAGACACAAATTCACAGGGAAGAAAGTGACTGAAGAGCT
  TCTGACCGATAATAGATACTTGCTTCTGGTTCTGATGGATGCTGAAAGAGCCTGGAGCTACGCCATGCAG
  CTGAAACAGGAAGCCAACACTGAACCCCGAAAACGGTTTCACTTGTTATCTCGCCTACGCAAAGCCGTGA
  AGCATGCAGAGGAATTGGAACGCTTGTGTGAGAGCAATCGCGTGGATGCCAAGACCAAATTAGAGGCTCA
  GGCTTACACAGCTTACCTCTCAGGAATGCTACGTTTTGAACATCAAGAATGGAAAGCTGCCATTGAGGCT
  TTTAACAAATGCAAAACTATCTATGAGAAGCTAGCCAGTGCTTTCACAGAGGAGCAGGCTGTGCTGTATA
  ACCAACGTGTGGAAGAGATTTCACCCAACATCCGCTATTGTGCATATAATATTGGGGACCAGTCAGCCAT
  CAATGAACTCATGCAGATGAGATTGAGGTCTGGGGGCACTGAGGGTCTCTTGGCTGAAAAATTGGAGGCT
  TTGATCACTCAGACTCGAGCCAAACAGGCAGCTACCATGAGTGAAGTGGAGTGGAGAGGGAGAACGGTTC
  CAGTGAAGATTGACAAAGTGCGCATTTTCTTATTAGGACTGGCTGATAACGAAGCAGCTATTGTCCAGGC
  TGAAAGCGAAGAAACTAAGGAGCGCCTGTTTGAATCAATGCTCAGCGAGTGTCGGGACGCCATCCAGGTG
  GTTCGGGAGGAGCTCAAGCCAGATCAGAAACAGAGAGATTATATCCTTGAAGGAGAGCCAGGGAAGGTGT
  CTAATCTTCAATACTTGCATAGCTACCTGACTTACATCAAGCTATCAACGGCAATCAAGCGTAATGAGAA
  CATGGCCAAAGGTCTGCAGAGGGCTCTGCTGCAGCAGCAGCCAGAGGATGACAGCAAGCGCTCACCCCGG
  CCCCAGGACCTGATCCGACTCTATGACATCATCTTACAGAATCTGGTGGAATTGCTCCAGCTTCCTGGTT
  TAGAGGAAGACAAAGCCTTCCAGAAAGAGATAGGCCTCAAGACTCTGGTGTTCAAAGCTTACAGGTGTTT
  TTTCATTGCTCAGTCCTATGTGCTGGTGAAGAAGTGGAGCGAAGCCCTTGTCCTGTATGACAGAGTCCTG
  AAATATGCAAATGAAGTAAATTCTGATGCTGGCGCCTTCAAGAACAGCCTAAAGGACCTGCCTGATGTGC
  AAGAGCTCATCACTCAAGTGCGGTCAGAGAAGTGCTCCCTGCAGGCCGCAGCCATCCTTGATGCAAACGA
  CGCTCATCAAACAGAGACCTCCTCCTCCCAAGTCAAGGACAATAAGCCTCTGGTTGAACGGTTTGAGACA
  TTCTGCCTGGACCCTTCCCTTGTCACCAAGCAAGCCAACCTTGTGCACTTCCCACCAGGCTTCCAGCCCA
  TTCCCTGCAAGCCTTTGTTCTTTGACCTGGCCCTCAACCATGTGGCTTTCCCACCCCTTGAGGACAAGTT
  GGAACAGAAGACCAAGAGTGGCCTCACTGGATACATCAAGGGCATCTTTGGATTCAGGAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214724 representing NM_014230
  Red=Cloning site Green=Tags(s)
  
  MAAEKQVPGGGGGGGSGGGGGSGGGGSGGGRGAGGEENKENERPSAGSKANKEFGDSLSLEILQIIKESQ
  QQHGLRHGDFQRYRGYCSRRQRRLRKTLNFKMGNRHKFTGKKVTEELLTDNRYLLLVLMDAERAWSYAMQ
  LKQEANTEPRKRFHLLSRLRKAVKHAEELERLCESNRVDAKTKLEAQAYTAYLSGMLRFEHQEWKAAIEA
  FNKCKTIYEKLASAFTEEQAVLYNQRVEEISPNIRYCAYNIGDQSAINELMQMRLRSGGTEGLLAEKLEA
  LITQTRAKQAATMSEVEWRGRTVPVKIDKVRIFLLGLADNEAAIVQAESEETKERLFESMLSECRDAIQV
  VREELKPDQKQRDYILEGEPGKVSNLQYLHSYLTYIKLSTAIKRNENMAKGLQRALLQQQPEDDSKRSPR
  PQDLIRLYDIILQNLVELLQLPGLEEDKAFQKEIGLKTLVFKAYRCFFIAQSYVLVKKWSEALVLYDRVL
  KYANEVNSDAGAFKNSLKDLPDVQELITQVRSEKCSLQAAAILDANDAHQTETSSSQVKDNKPLVERFET
  FCLDPSLVTKQANLVHFPPGFQPIPCKPLFFDLALNHVAFPPLEDKLEQKTKSGLTGYIKGIFGFRS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014230
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014230.4
• RefSeq Size: 2515 bp
• RefSeq ORF: 1884 bp
• Locus ID: 6730
• UniProt ID: Q9UHB9
• Cytogenetics: 17q25.1
• Protein Pathways: Protein export
• Gene Summary: This gene encodes a subunit of the signal recognition particle (SRP). The SRP is a ribonucleoprotein complex that transports secreted and membrane proteins to the endoplasmic reticulum for processing. The complex includes a 7S RNA and six protein subunits. The encoded protein is the 68kDa component of the SRP, and forms a heterodimer with the 72kDa subunit that is required for SRP function. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and three pseudogenes of this gene are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, May 2012]