USP19 (NM_006677) Human Tagged ORF Clone

CAT#: RG214190

USP19 (tGFP-tagged) - Human ubiquitin specific peptidase 19 (USP19), transcript variant 4

Product Images

Circular map for RG214190

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: USP19
• Synonyms: ZMYND9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214190 representing NM_006677
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGCGGGGCCAGTGCCACAGGCCCAAGGAGAGGGCCCCCAGGACTGGAGGACACCACTAGTAAGA
  AGAAGCAGAAGGATCGAGCAAACCAGGAGAGCAAGGATGGAGATCCTAGGAAAGAGACAGGGTCTCGATA
  TGTTGCCCAGGCTGGTCTTGAACCTCTGGCCTCAGGTGATCCTTCTGCCTCAGCCTCCCATGCAGCTGGG
  ATCACAGGCTCACGCCACCGTACCCGGCTGTTCTTTCCTTCATCGTCAGGGTCAGCATCCACTCCTCAAG
  AGGAGCAGACCAAAGAGGGAGCTTGTGAAGACCCTCATGATCTCTTGGCTACTCCCACTCCAGAGTTGTT
  GCTCGATTGGAGGCAGAGTGCAGAAGAGGTGATTGTCAAGCTTCGTGTGGGAGTAGGTCCCCTGCAGCTG
  GAGGATGTAGATGCTGCTTTCACAGATACAGACTGTGTGGTGCGGTTTGCAGGTGGTCAGCAGTGGGGTG
  GTGTCTTCTATGCTGAGATAAAAAGCTCTTGTGCTAAAGTGCAAACCCGCAAGGGCAGTCTCCTGCACCT
  GACACTGCCCAAAAAGGTGCCTATGCTCACGTGGCCCTCCCTCCTGGTTGAGGCTGATGAACAGCTTTGC
  ATACCACCGCTGAACTCCCAAACCTGCCTCCTGGGCTCAGAGGAGAATTTAGCCCCTTTGGCAGGAGAGA
  AAGCAGTGCCTCCCGGGAATGACCCAGTCTCTCCAGCCATGGTCCGGAGCAGAAACCCTGGGAAAGATGA
  CTGTGCCAAGGAGGAGATGGCAGTGGCAGCAGATGCTGCAACCTTGGTGGATGAGCCCGAGTCGATGGTG
  AACCTGGCGTTTGTCAAGAATGACTCGTATGAGAAGGGCCCGGATTCAGTGGTGGTGCACGTGTACGTGA
  AGGAGATCTGCAGGGACACCTCAAGAGTACTTTTCCGTGAGCAGGACTTCACGCTCATCTTCCAGACCAG
  GGATGGAAACTTCCTGAGGCTGCACCCGGGCTGTGGGCCCCACACCACCTTCCGTTGGCAGGTGAAGCTC
  AGGAATCTGATTGAGCCAGAGCAGTGCACCTTCTGTTTCACGGCTTCTCGCATCGACATCTGCCTTCGTA
  AGAGGCAGAGTCAGCGCTGGGGGGGCCTGGAGGCCCCGGCTGCACGAGTGGGTGGTGCAAAGGTTGCCGT
  GCCGACAGGTCCAACCCCTCTGGATTCAACCCCACCAGGAGGTGCTCCCCACCCCCTGACAGGCCAGGAG
  GAGGCCCGGGCTGTGGAGAAGGATAAATCCAAGGCACGATCTGAGGACACAGGGCTAGACAGTGTGGCAA
  CCCGCACACCCATGGAGCATGTAACCCCAAAGCCAGAGACACACCTGGCCTCGCCCAAGCCTACATGCAT
  GGTGCCTCCCATGCCCCACAGCCCAGTTAGTGGAGACAGCGTGGAGGAGGAGGAAGAGGAAGAGAAGAAG
  GTGTGTCTGCCAGGCTTCACTGGCCTTGTCAATTTAGGCAACACCTGCTTCATGAACAGCGTCATTCAGT
  CTCTGTCCAACACTCGGGAACTCCGGGACTTCTTCCATGACCGCTCCTTTGAGGCTGAGATCAACTACAA
  CAACCCACTAGGGACTGGTGGGCGTCTGGCCATTGGCTTTGCCGTGCTGCTTCGGGCGCTGTGGAAGGGC
  ACCCACCATGCCTTCCAGCCTTCCAAGTTGAAGGCCATTGTGGCGAGTAAGGCCAGCCAGTTCACAGGCT
  ATGCACAGCATGATGCCCAGGAGTTCATGGCTTTCCTGCTGGATGGGCTGCACGAGGACCTGAATCGCAT
  TCAGAACAAGCCCTACACAGAGACCGTGGATTCAGATGGGCGGCCCGATGAGGTGGTAGCTGAGGAAGCA
  TGGCAGCGGCACAAGATGAGGAATGACTCTTTCATCGTGGACCTATTTCAGGGGCAGTACAAGTCGAAGC
  TGGTGTGCCCTGTGTGTGCCAAGGTCTCCATCACTTTTGACCCGTTTCTTTATCTGCCGGTGCCCTTGCC
  ACAAAAGCAAAAGGTTCTCCCTGTCTTTTATTTTGCCCGAGAGCCCCACAGCAAGCCCATCAAGTTCCTG
  GTGAGCGTCAGCAAGGAGAACTCCACTGCGAGCGAAGTATTGGACTCCCTCTCTCAGAGTGTTCATGTGA
  AGCCTGAGAACCTGCGTTTGGCGGAGGTAATTAAGAATCGTTTTCATCGTGTGTTCCTACCCTCCCACTC
  ACTGGACACTGTGTCCCCATCTGATACGCTCCTCTGCTTTGAGCTGCTATCCTCAGAGTTGGCTAAGGAG
  CGGGTAGTGGTGCTAGAGGTGCAACAGCGCCCCCAGGTGCCCAGCGTCCCCATCTCCAAGTGTGCAGCCT
  GCCAGCGGAAGCAACAGTCGGAGGATGAAAAGCTGAAGCGCTGTACCCGGTGCTACCGTGTGGGCTACTG
  CAACCAGCTCTGCCAGAAAACCCACTGGCCTGACCACAAGGGCCTCTGCCGACCTGAGAACATTGGCTAC
  CCCTTCCTGGTCAGTGTACCTGCCTCACGCCTCACTTATGCCCGCCTCGCTCAGTTGCTAGAGGGCTATG
  CCCGGTACTCTGTGAGTGTATTCCAGCCACCCTTTCAGCCAGGCCGCATGGCCTTGGAGTCTCAGAGCCC
  TGGCTGCACCACACTGCTCTCCACAGGTTCCCTGGAGGCTGGGGACAGCGAGAGAGACCCCATTCAGCCA
  CCTGAGCTCCAGCTGGTGACCCCTATGGCTGAGGGGGACACAGGGCTTCCCCGGGTGTGGGCAGCCCCTG
  ACCGGGGTCCTGTGCCCAGCACCAGTGGAATTTCTTCTGAGATGCTGGCCAGTGGGCCCATTGAGGTTGG
  CTCCTTGCCAGCTGGCGAGAGGGTGTCCCGACCCGAAGCTGCTGTGCCTGGGTACCAGCATCCAAGTGAA
  GCTATGAATGCCCACACACCCCAGTTCTTCATCTATAAAATTGATTCATCCAACCGAGAGCAGCGGCTAG
  AGGACAAAGGAGACACCCCACTGGAGCTGGGTGACGACTGTAGCCTGGCTCTCGTCTGGCGGAACAATGA
  GCGCTTGCAGGAGTTTGTGTTGGTAGCCTCCAAGGAGCTGGAATGTGCTGAGGATCCAGGCTCTGCCGGT
  GAGGCTGCCCGGGCCGGCCACTTCACCCTGGACCAGTGCCTCAACCTCTTCACACGGCCTGAGGTGCTGG
  CACCCGAGGAGGCCTGGTACTGCCCACAGTGCAAACAGCACCGTGAGGCCTCCAAGCAGCTGTTGCTATG
  GCGCCTGCCAAATGTTCTCATCGTGCAGCTCAAGCGCTTCTCCTTTCGTAGTTTTATCTGGCGTGACAAG
  ATCAATGACTTGGTGGAGTTCCCTGTTAGGAACCTGGACCTGAGCAAGTTCTGCATTGGTCAGAAAGAGG
  AGCAGCTGCCCAGCTACGATCTATATGCTGTCATCAACCACTATGGAGGCATGATTGGTGGCCACTACAC
  TGCCTGTGCACGCCTGCCCAATGATCGTAGCAGTCAGCGCAGTGACGTGGGCTGGCGCTTGTTTGATGAC
  AGCACAGTGACAACGGTAGACGAGAGCCAGGTTGTGACGCGTTATGCCTATGTACTCTTCTACCGCCGGC
  GGAACTCTCCTGTGGAGAGGCCCCCCAGGGCAGGTCACTCTGAGCACCACCCAGACCTAGGCCCTGCAGC
  TGAGGCTGCTGCCAGCCAGGCTTCCCGGATTTGGCAGGAGCTGGAGGCTGAGGAGGAGCCGGTGCCTGAG
  GGGTCTGGGCCCCTGGGTCCCTGGGGGCCCCAAGACTGGGTGGGCCCCCTACCACGTGGCCCTACCACAC
  CAGATGAGGGCTGCCTCCGGTACTTTGTCCTGGGCACCGTGGCGGCTTTGGTGGCCCTCGTGCTCAACGT
  GTTCTATCCTCTGGTATCCCAGAGTCGCTGGAGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214190 representing NM_006677
  Red=Cloning site Green=Tags(s)
  
  MSGGASATGPRRGPPGLEDTTSKKKQKDRANQESKDGDPRKETGSRYVAQAGLEPLASGDPSASASHAAG
  ITGSRHRTRLFFPSSSGSASTPQEEQTKEGACEDPHDLLATPTPELLLDWRQSAEEVIVKLRVGVGPLQL
  EDVDAAFTDTDCVVRFAGGQQWGGVFYAEIKSSCAKVQTRKGSLLHLTLPKKVPMLTWPSLLVEADEQLC
  IPPLNSQTCLLGSEENLAPLAGEKAVPPGNDPVSPAMVRSRNPGKDDCAKEEMAVAADAATLVDEPESMV
  NLAFVKNDSYEKGPDSVVVHVYVKEICRDTSRVLFREQDFTLIFQTRDGNFLRLHPGCGPHTTFRWQVKL
  RNLIEPEQCTFCFTASRIDICLRKRQSQRWGGLEAPAARVGGAKVAVPTGPTPLDSTPPGGAPHPLTGQE
  EARAVEKDKSKARSEDTGLDSVATRTPMEHVTPKPETHLASPKPTCMVPPMPHSPVSGDSVEEEEEEEKK
  VCLPGFTGLVNLGNTCFMNSVIQSLSNTRELRDFFHDRSFEAEINYNNPLGTGGRLAIGFAVLLRALWKG
  THHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHEDLNRIQNKPYTETVDSDGRPDEVVAEEA
  WQRHKMRNDSFIVDLFQGQYKSKLVCPVCAKVSITFDPFLYLPVPLPQKQKVLPVFYFAREPHSKPIKFL
  VSVSKENSTASEVLDSLSQSVHVKPENLRLAEVIKNRFHRVFLPSHSLDTVSPSDTLLCFELLSSELAKE
  RVVVLEVQQRPQVPSVPISKCAACQRKQQSEDEKLKRCTRCYRVGYCNQLCQKTHWPDHKGLCRPENIGY
  PFLVSVPASRLTYARLAQLLEGYARYSVSVFQPPFQPGRMALESQSPGCTTLLSTGSLEAGDSERDPIQP
  PELQLVTPMAEGDTGLPRVWAAPDRGPVPSTSGISSEMLASGPIEVGSLPAGERVSRPEAAVPGYQHPSE
  AMNAHTPQFFIYKIDSSNREQRLEDKGDTPLELGDDCSLALVWRNNERLQEFVLVASKELECAEDPGSAG
  EAARAGHFTLDQCLNLFTRPEVLAPEEAWYCPQCKQHREASKQLLLWRLPNVLIVQLKRFSFRSFIWRDK
  INDLVEFPVRNLDLSKFCIGQKEEQLPSYDLYAVINHYGGMIGGHYTACARLPNDRSSQRSDVGWRLFDD
  STVTTVDESQVVTRYAYVLFYRRRNSPVERPPRAGHSEHHPDLGPAAEAAASQASRIWQELEAEEEPVPE
  GSGPLGPWGPQDWVGPLPRGPTTPDEGCLRYFVLGTVAALVALVLNVFYPLVSQSRWR
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006677
• ORF Size: 3954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006677.2
• RefSeq Size: 4401 bp
• RefSeq ORF: 3957 bp
• Locus ID: 10869
• UniProt ID: O94966
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome, Protease, Transmembrane
• Gene Summary: Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This protein is a ubiquitin protein ligase and plays a role in muscle wasting. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2017]