USP19 (NM_006677) Human Untagged Clone

CAT#: SC309922

USP19 (untagged)-Human ubiquitin specific peptidase 19 (USP19), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP19
• Synonyms: ZMYND9
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006677 edited
  ATGTCTGGCGGGGCCAGTGCCACAGGCCCAAGGAGAGGGCCCCCAGGACTGGAGGACACC
  ACTAGTAAGAAGAAGCAGAAGGATCGAGCAAACCAGGAGAGCAAGGATGGAGATCCTAGG
  AAAGAGACAGGGTCTCGATATGTTGCCCAGGCTGGTCTTGAACCTCTGGCCTCAGGTGAT
  CCTTCTGCCTCAGCCTCCCATGCAGCTGGGATCACAGGCTCACGCCACCGTACCCGGCTG
  TTCTTTCCTTCATCGTCAGGGTCAGCATCCACTCCTCAAGAGGAGCAGACCAAAGAGGGA
  GCTTGTGAAGACCCTCATGATCTCTTGGCTACTCCCACTCCAGAGTTGTTGCTCGATTGG
  AGGCAGAGTGCAGAAGAGGTGATTGTCAAGCTTCGTGTGGGAGTAGGTCCCCTGCAGCTG
  GAGGATGTAGATGCTGCTTTCACAGATACAGACTGTGTGGTGCGGTTTGCAGGTGGTCAG
  CAGTGGGGTGGTGTCTTCTATGCTGAGATAAAAAGCTCTTGTGCTAAAGTGCAAACCCGC
  AAGGGCAGTCTCCTGCACCTGACACTGCCCAAAAAGGTGCCTATGCTCACGTGGCCCTCC
  CTCCTGGTTGAGGCTGATGAACAGCTTTGCATACCACCGCTGAACTCCCAAACCTGCCTC
  CTGGGCTCAGAGGAGAATTTAGCCCCTTTGGCAGGAGAGAAAGCAGTGCCTCCCGGGAAT
  GACCCAGTCTCTCCAGCCATGGTCCGGAGCAGAAACCCTGGGAAAGATGACTGTGCCAAG
  GAGGAGATGGCAGTGGCAGCAGATGCTGCAACCTTGGTGGATGAGCCCGAGTCGATGGTG
  AACCTGGCGTTTGTCAAGAATGACTCGTATGAGAAGGGCCCGGATTCAGTGGTGGTGCAC
  GTGTACGTGAAGGAGATCTGCAGGGACACCTCAAGAGTACTTTTCCGTGAGCAGGACTTC
  ACGCTCATCTTCCAGACCAGGGATGGAAACTTCCTGAGGCTGCACCCGGGCTGTGGGCCC
  CACACCACCTTCCGTTGGCAGGTGAAGCTCAGGAATCTGATTGAGCCAGAGCAGTGCACC
  TTCTGTTTCACGGCTTCTCGCATCGACATCTGCCTTCGTAAGAGGCAGAGTCAGCGCTGG
  GGGGGCCTGGAGGCCCCGGCTGCACGAGGTGCAGTGGGTGGTGCAAAGGTTGCCGTGCCG
  ACAGGTCCAACCCCTCTGGATTCAACCCCACCAGGAGGTGCTCCCCACCCCCTGACAGGC
  CAGGAGGAGGCCCGGGCTGTGGAGAAGGATAAATCCAAGGCACGATCTGAGGACACAGGG
  CTAGACAGTGTGGCAACCCGCACACCCATGGAGCATGTAACCCCAAAGCCAGAGACACAC
  CTGGCCTCGCCCAAGCCTACATGCATGGTGCCTCCCATGCCCCACAGCCCAGTTAGTGGA
  GACAGCGTGGAGGAGGAGGAAGAGGAAGAGAAGAAGGTGTGTCTGCCAGGCTTCACTGGC
  CTTGTCAATTTAGGCAACACCTGCTTCATGAACAGCGTCATTCAGTCTCTGTCCAACACT
  CGGGAACTCCGGGACTTCTTCCATGACCGCTCCTTTGAGGCTGAGATCAACTACAACAAC
  CCACTAGGGACTGGTGGGCGTCTGGCCATTGGCTTTGCCGTGCTGCTTCGGGCGCTGTGG
  AAGGGCACCCACCATGCCTTCCAGCCTTCCAAGTTGAAGGCCATTGTGGCGAGTAAGGCC
  AGCCAGTTCACAGGCTATGCACAGCATGATGCCCAGGAGTTCATGGCTTTCCTGCTGGAT
  GGGCTGCACGAGGACCTGAATCGCATTCAGAACAAGCCCTACACAGAGACCGTGGATTCA
  GATGGGCGGCCCGATGAGGTGGTAGCTGAGGAAGCATGGCAGCGGCACAAGATGAGGAAT
  GACTCTTTCATCGTGGACCTATTTCAGGGGCAGTACAAGTCGAAGCTGGTGTGCCCTGTG
  TGTGCCAAGGTCTCCATCACTTTTGACCCGTTTCTTTATCTGCCGGTGCCCTTGCCACAA
  AAGCAAAAGGTTCTCCCTGTCTTTTATTTTGCCCGAGAGCCCCACAGCAAGCCCATCAAG
  TTCCTGGTGAGCGTCAGCAAGGAGAACTCCACTGCGAGCGAAGTATTGGACTCCCTCTCT
  CAGAGTGTTCATGTGAAGCCTGAGAACCTGCGTTTGGCGGAGGTAATTAAGAATCGTTTT
  CATCGTGTGTTCCTACCCTCCCACTCACTGGACACTGTGTCCCCATCTGATACGCTCCTC
  TGCTTTGAGCTGCTATCCTCAGAGTTGGCTAAGGAGCGGGTAGTGGTGCTAGAGGTGCAA
  CAGCGCCCCCAGGTGCCCAGCGTCCCCATCTCCAAGTGTGCAGCCTGCCAGCGGAAGCAA
  CAGTCGGAGGATGAAAAGCTGAAGCGCTGTACCCGGTGCTACCGTGTGGGCTACTGCAAC
  CAGCTCTGCCAGAAAACCCACTGGCCTGACCACAAGGGCCTCTGCCGACCTGAGAACATT
  GGCTACCCCTTCCTGGTCAGTGTACCTGCCTCACGCCTCACTTATGCCCGCCTCGCTCAG
  TTGCTAGAGGGCTATGCCCGGTACTCTGTGAGTGTATTCCAGCCACCCTTTCAGCCAGGC
  CGCATGGCCTTGGAGTCTCAGAGCCCTGGCTGCACCACACTGCTCTCCACAGGTTCCCTG
  GAGGCTGGGGACAGCGAGAGAGACCCCATTCAGCCACCTGAGCTCCAGCTGGTGACCCCT
  ATGGCTGAGGGGGACACAGGGCTTCCCCGGGTGTGGGCAGCCCCTGACCGGGGTCCTGTG
  CCCAGCACCAGTGGAATTTCTTCTGAGATGCTGGCCAGTGGGCCCATTGAGGTTGGCTCC
  TTGCCAGCTGGCGAGAGGGTGTCCCGACCCGAAGCTGCTGTGCCTGGGTACCAGCATCCA
  AGTGAAGCTATGAATGCCCACACACCCCAGTTCTTCATCTATAAAATTGATTCATCCAAC
  CGAGAGCAGCGGCTAGAGGACAAAGGAGACACCCCACTGGAGCTGGGTGACGACTGTAGC
  CTGGCTCTCGTCTGGCGGAACAATGAGCGCTTGCAGGAGTTTGTGTTGGTAGCCTCCAAG
  GAGCTGGAATGTGCTGAGGATCCAGGCTCTGCCGGTGAGGCTGCCCGGGCCGGCCACTTC
  ACCCTGGACCAGTGCCTCAACCTCTTCACACGGCCTGAGGTGCTGGCACCCGAGGAGGCC
  TGGTACTGCCCACAGTGCAAACAGCACCGTGAGGCCTCCAAGCAGCTGTTGCTATGGCGC
  CTGCCAAATGTTCTCATCGTGCAGCTCAAGCGCTTCTCCTTTCGTAGTTTTATCTGGCGT
  GACAAGATCAATGACTTGGTGGAGTTCCCTGTTAGGAACCTGGACCTGAGCAAGTTCTGC
  ATTGGTCAGAAAGAGGAGCAGCTGCCCAGCTACGATCTATATGCTGTCATCAACCACTAT
  GGAGGCATGATTGGTGGCCACTACACTGCCTGTGCACGCCTGCCCAATGATCGTAGCAGT
  CAGCGCAGTGACGTGGGCTGGCGCTTGTTTGATGACAGCACAGTGACAACGGTAGACGAG
  AGCCAGGTTGTGACGCGTTATGCCTATGTACTCTTCTACCGCCGGCGGAACTCTCCTGTG
  GAGAGGCCCCCCAGGGCAGGTCACTCTGAGCACCACCCAGACCTAGGCCCTGCAGCTGAG
  GCTGCTGCCAGCCAGGCTTCCCGGATTTGGCAGGAGCTGGAGGCTGAGGAGGAGCCGGTG
  CCTGAGGGGTCTGGGCCCCTGGGTCCCTGGGGGCCCCAAGACTGGGTGGGCCCCCTACCA
  CGTGGCCCTACCACACCAGATGAGGGCTGCCTCCGGTACTTTGTCCTGGGCACCGTGGCG
  GCTTTGGTGGCCCTCGTGCTCAACGTGTTCTATCCTCTGGTATCCCAGAGTCGCTGGAGA
  TGA
  
• Restriction Sites: Please inquire
• ACCN: NM_006677
• Insert Size: 4600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006677.1, NP_006668.1
• RefSeq Size: 4401 bp
• RefSeq ORF: 3957 bp
• Locus ID: 10869
• UniProt ID: O94966
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome, Protease, Transmembrane
• Gene Summary: Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This protein is a ubiquitin protein ligase and plays a role in muscle wasting. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2017]
  Transcript Variant: This variant (4) lacks an in-frame exon in the CDS, as compared to variant 1. The resulting isoform (4) lacks an internal segment, as compared to isoform 1.