TRPC6 (NM_004621) Human Tagged ORF Clone

CAT#: RG210783

TRPC6 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily C, member 6 (TRPC6)

Product Images

Circular map for RG210783

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TRPC6
• Synonyms: FSGS2; TRP6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210783 representing NM_004621
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAGAGCCCGGCGTTCGGGCCCCGGAGGGGCAGTTCTCCCCGGGGCGCTGCCGGAGCCGCTGCGC
  GGCGCAACGAGAGCCAGGACTATCTGCTCATGGACTCGGAGCTGGGAGAAGACGGCTGCCCGCAAGCCCC
  GCTGCCTTGCTACGGCTACTACCCCTGCTTCCGGGGATCTGACAACAGACTGGCTCACCGGCGGCAGACA
  GTTCTCCGTGAGAAGGGGAGAAGGTTAGCTAATCGAGGACCAGCATACATGTTTAGTGATCGCTCCACAA
  GCCTATCTATAGAGGAGGAACGCTTTTTGGATGCAGCTGAATATGGTAACATCCCAGTGGTGCGGAAGAT
  GTTAGAAGAATGCCACTCACTCAACGTTAACTGTGTGGATTACATGGGCCAGAATGCCCTACAGTTGGCA
  GTGGCCAATGAGCATCTGGAAATTACAGAACTTCTTCTCAAGAAAGAAAACCTCTCTCGAGTTGGGGATG
  CTTTGCTTCTAGCTATTAGTAAAGGTTATGTTCGGATTGTGGAAGCAATTCTCAGTCATCCGGCTTTTGC
  TGAAGGCAAGAGGTTAGCAACCAGCCCTAGCCAGTCTGAACTCCAGCAAGATGATTTTTATGCCTATGAT
  GAAGATGGGACACGGTTCTCCCATGATGTGACTCCAATCATTCTGGCTGCCCACTGCCAGGAATATGAAA
  TTGTGCATACCCTCCTGCGGAAGGGTGCTAGGATTGAACGGCCTCATGATTATTTCTGCAAGTGCAATGA
  CTGCAACCAGAAACAGAAGCATGACTCGTTTAGCCACTCCAGATCTAGGATTAATGCCTATAAAGGCCTG
  GCAAGTCCGGCTTACCTGTCATTGTCTAGTGAAGATCCAGTCATGACGGCTTTAGAACTTAGCAATGAAC
  TGGCAGTTCTGGCCAATATTGAGAAAGAGTTCAAGAATGACTACAAAAAACTGTCAATGCAGTGCAAAGA
  CTTTGTTGTTGGACTCCTTGATCTGTGCAGAAACACTGAAGAAGTCGAGGCCATTCTGAATGGGGATGTT
  GAAACGCTCCAGAGTGGTGATCACGGTCGCCCAAATCTCAGCCGTTTAAAACTTGCCATTAAATATGAAG
  TAAAAAAATTTGTAGCTCATCCAAACTGCCAACAGCAACTTCTCTCCATTTGGTATGAGAATCTTTCTGG
  TTTACGACAGCAGACAATGGCGGTCAAGTTCCTTGTGGTCCTTGCTGTTGCCATTGGACTGCCCTTCCTG
  GCTCTCATTTACTGGTTTGCTCCATGCAGCAAGATGGGGAAGATAATGCGTGGACCATTCATGAAGTTTG
  TAGCACACGCAGCCTCCTTCACCATTTTTCTGGGACTGCTAGTCATGAATGCAGCTGACAGATTTGAAGG
  CACAAAACTCCTTCCTAATGAAACCAGCACAGATAATGCAAAACAGCTGTTCAGGATGAAAACATCCTGC
  TTCTCATGGATGGAGATGCTCATTATATCCTGGGTAATAGGCATGATATGGGCTGAATGTAAAGAAATCT
  GGACTCAGGGCCCCAAGGAATATTTGTTTGAGTTGTGGAACATGCTTGATTTTGGTATGTTAGCAATTTT
  CGCAGCATCATTCATTGCGAGATTCATGGCATTTTGGCATGCTTCCAAAGCCCAGAGCATCATTGACGCA
  AACGATACTTTGAAGGACTTGACGAAAGTAACATTGGGAGACAATGTGAAATACTACAATTTGGCCAGGA
  TAAAGTGGGACCCCTCTGATCCTCAAATAATATCTGAAGGTCTTTATGCAATTGCTGTAGTTTTAAGTTT
  CTCTAGGATAGCTTATATTTTACCAGCAAATGAAAGCTTTGGACCTCTGCAGATATCACTTGGAAGAACA
  GTCAAAGACATCTTCAAGTTCATGGTCATATTCATTATGGTGTTTGTGGCCTTTATGATTGGAATGTTCA
  ATCTCTACTCCTACTACATTGGTGCAAAACAAAATGAAGCCTTCACAACAGTTGAAGAGAGTTTTAAGAC
  ACTGTTCTGGGCTATATTTGGACTTTCTGAAGTGAAATCAGTGGTCATCAACTATAACCACAAATTCATT
  GAAAACATTGGTTACGTTCTTTATGGAGTCTATAATGTTACGATGGTCATTGTTTTGCTAAATATGTTAA
  TTGCCATGATCAACAGTTCATTCCAGGAAATTGAGGATGACGCTGATGTGGAGTGGAAATTTGCAAGGGC
  CAAACTCTGGTTTTCCTACTTTGAGGAGGGCAGAACACTTCCTGTACCCTTCAATCTGGTGCCGAGTCCA
  AAGTCCCTGTTTTATCTCTTACTGAAGCTTAAAAAATGGATTTCTGAGCTGTTCCAGGGCCATAAAAAAG
  GTTTCCAGGAAGATGCAGAGATGAACAAGATAAATGAAGAAAAGAAACTTGGAATTTTAGGAAGTCATGA
  AGACCTTTCAAAATTATCACTTGACAAAAAACAGGTTGGGCACAATAAACAACCAAGTATAAGGAGCTCA
  GAAGATTTCCATCTAAATAGTTTCAATAATCCTCCAAGACAATATCAGAAAATAATGAAAAGGCTCATTA
  AAAGATATGTACTGCAGGCCCAGATAGATAAGGAGAGTGATGAAGTGAACGAAGGGGAACTGAAGGAAAT
  TAAGCAGGACATCTCAAGTCTCCGCTATGAACTCCTTGAAGAAAAATCTCAGAATACAGAAGACCTAGCA
  GAACTTATTAGAGAACTTGGAGAGAAATTATCCATGGAACCAAATCAAGAGGAAACCAATAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210783 representing NM_004621
  Red=Cloning site Green=Tags(s)
  
  MSQSPAFGPRRGSSPRGAAGAAARRNESQDYLLMDSELGEDGCPQAPLPCYGYYPCFRGSDNRLAHRRQT
  VLREKGRRLANRGPAYMFSDRSTSLSIEEERFLDAAEYGNIPVVRKMLEECHSLNVNCVDYMGQNALQLA
  VANEHLEITELLLKKENLSRVGDALLLAISKGYVRIVEAILSHPAFAEGKRLATSPSQSELQQDDFYAYD
  EDGTRFSHDVTPIILAAHCQEYEIVHTLLRKGARIERPHDYFCKCNDCNQKQKHDSFSHSRSRINAYKGL
  ASPAYLSLSSEDPVMTALELSNELAVLANIEKEFKNDYKKLSMQCKDFVVGLLDLCRNTEEVEAILNGDV
  ETLQSGDHGRPNLSRLKLAIKYEVKKFVAHPNCQQQLLSIWYENLSGLRQQTMAVKFLVVLAVAIGLPFL
  ALIYWFAPCSKMGKIMRGPFMKFVAHAASFTIFLGLLVMNAADRFEGTKLLPNETSTDNAKQLFRMKTSC
  FSWMEMLIISWVIGMIWAECKEIWTQGPKEYLFELWNMLDFGMLAIFAASFIARFMAFWHASKAQSIIDA
  NDTLKDLTKVTLGDNVKYYNLARIKWDPSDPQIISEGLYAIAVVLSFSRIAYILPANESFGPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004621
• ORF Size: 2793 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004621.2
• RefSeq Size: 4612 bp
• RefSeq ORF: 2796 bp
• Locus ID: 7225
• UniProt ID: Q9Y210
• Cytogenetics: 11q22.1
• Domains: ANK, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). [provided by RefSeq, Mar 2009]