12 Lipoxygenase (ALOX12) (NM_000697) Human Tagged ORF Clone

CAT#: RG210211

ALOX12 (tGFP-tagged) - Human arachidonate 12-lipoxygenase (ALOX12)

Product Images

Circular map for RG210211

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: 12 Lipoxygenase
• Synonyms: 12-LOX; 12S-LOX; LOG12
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210211 representing NM_000697
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGCTACCGCATCCGCGTGGCCACCGGGGCCTGGCTCTTCTCCGGGTCGTACAACCGCGTGCAGC
  TTTGGCTGGTCGGGACGCGCGGGGAGGCGGAGCTGGAGCTGCAGCTGCGGCCGGCGCGGGGCGAGGAGGA
  GGAGTTTGATCATGACGTTGCAGAGGACTTGGGGCTCCTGCAGTTCGTGAGGCTGCGCAAGCACCACTGG
  CTGGTGGACGACGCGTGGTTCTGCGACCGCATCACGGTGCAGGGCCCTGGAGCCTGCGCGGAGGTGGCCT
  TCCCGTGCTACCGCTGGGTGCAGGGCGAGGACATCCTGAGCCTGCCCGAGGGCACCGCCCGCCTGCCAGG
  AGACAATGCTTTGGACATGTTCCAGAAGCATCGAGAGAAGGAACTGAAAGACAGACAGCAGATCTACTGC
  TGGGCCACCTGGAAGGAAGGGTTACCCCTGACCATCGCTGCAGACCGTAAGGATGATCTACCTCCAAATA
  TGAGATTCCATGAGGAGAAGAGGCTGGACTTTGAATGGACACTGAAGGCAGGGGCTCTGGAGATGGCCCT
  CAAACGTGTTTACACCCTCCTGAGCTCCTGGAACTGCCTAGAAGACTTTGATCAGATCTTCTGGGGCCAG
  AAGAGTGCCCTGGCTGAGAAGGTTCGCCAGTGCTGGCAGGATGATGAGTTGTTCAGCTACCAGTTCCTCA
  ATGGTGCCAACCCCATGCTGTTGAGACGCTCGACCTCTCTGCCCTCCAGGCTAGTGCTGCCCTCAGGGAT
  GGAAGAGCTTCGGGCTCAACTGGAGAAAGAACTTCAGAATGGTTCCCTGTTTGAAGCTGACTTCATCCTT
  CTGGATGGAATTCCAGCCAACGTGATCCGAGGAGAGAAGCAATACCTGGCTGCCCCCCTCGTTATGCTGA
  AGATGGAGCCCAATGGGAAGCTGCAGCCCATGGTCATCCAGATTCAGCCTCCCAACCCCAGCTCTCCAAC
  CCCAACACTGTTCCTGCCCTCAGACCCCCCACTTGCCTGGCTCCTGGCAAAGTCCTGGGTCCGAAATTCA
  GATTTCCAACTGCACGAGATCCAGTATCACTTGCTGAACACGCACCTGGTGGCTGAGGTCATCGCTGTCG
  CCACCATGCGGTGCCTCCCAGGACTGCACCCCATCTTCAAGTTCCTGATCCCCCATATCCGCTACACCAT
  GGAAATCAACACCCGGGCCCGGACCCAACTCATCTCAGATGGAGGAATTTTTGATAAGGCAGTGAGCACA
  GGTGGAGGGGGCCATGTACAGTTGCTCCGTCGGGCGGCAGCTCAGCTGACCTACTGCTCCCTCTGTCCTC
  CTGACGACCTGGCTGACCGGGGCCTGCTGGGACTCCCAGGTGCTCTCTATGCCCATGATGCTTTACGGCT
  CTGGGAGATCATTGCCAGGTATGTGGAGGGGATCGTCCACCTCTTCTACCAGAGGGATGACATAGTGAAG
  GGGGACCCTGAGCTGCAGGCCTGGTGTCGGGAGATCACGGAGGTGGGGCTGTGCCAGGCCCAGGACCGAG
  GTTTCCCTGTCTCCTTCCAGTCCCAGAGTCAACTCTGCCATTTCCTCACCATGTGCGTCTTCACGTGCAC
  TGCCCAGCATGCCGCCATCAACCAGGGCCAGCTGGACTGGTATGCCTGGGTCCCTAATGCTCCATGCACA
  ATGCGGATGCCCCCACCCACCACCAAGGAAGATGTGACGATGGCCACAGTGATGGGGTCACTACCTGATG
  TCCGGCAGGCCTGTCTTCAAATGGCCATCTCATGGCATCTGAGTCGCCGCCAGCCAGACATGGTGCCTCT
  GGGGCACCACAAAGAAAAATATTTCTCAGGCCCCAAGCCCAAAGCTGTGCTAAACCAATTCCGAACAGAT
  TTGGAAAAGCTGGAAAAGGAGATTACAGCCCGGAATGAGCAACTTGACTGGCCCTATGAATATCTGAAGC
  CCAGCTGCATAGAGAACAGTGTCACCATC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210211 representing NM_000697
  Red=Cloning site Green=Tags(s)
  
  MGRYRIRVATGAWLFSGSYNRVQLWLVGTRGEAELELQLRPARGEEEEFDHDVAEDLGLLQFVRLRKHHW
  LVDDAWFCDRITVQGPGACAEVAFPCYRWVQGEDILSLPEGTARLPGDNALDMFQKHREKELKDRQQIYC
  WATWKEGLPLTIAADRKDDLPPNMRFHEEKRLDFEWTLKAGALEMALKRVYTLLSSWNCLEDFDQIFWGQ
  KSALAEKVRQCWQDDELFSYQFLNGANPMLLRRSTSLPSRLVLPSGMEELRAQLEKELQNGSLFEADFIL
  LDGIPANVIRGEKQYLAAPLVMLKMEPNGKLQPMVIQIQPPNPSSPTPTLFLPSDPPLAWLLAKSWVRNS
  DFQLHEIQYHLLNTHLVAEVIAVATMRCLPGLHPIFKFLIPHIRYTMEINTRARTQLISDGGIFDKAVST
  GGGGHVQLLRRAAAQLTYCSLCPPDDLADRGLLGLPGALYAHDALRLWEIIARYVEGIVHLFYQRDDIVK
  GDPELQAWCREITEVGLCQAQDRGFPVSFQSQSQLCHFLTMCVFTCTAQHAAINQGQLDWYAWVPNAPCT
  MRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRRQPDMVPLGHHKEKYFSGPKPKAVLNQFRTD
  LEKLEKEITARNEQLDWPYEYLKPSCIENSVTI
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000697
• ORF Size: 1989 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000697.1, NP_000688.1
• RefSeq Size: 2335 bp
• RefSeq ORF: 1992 bp
• Locus ID: 239
• UniProt ID: P18054
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on different polyunsaturated fatty acid substrates to generate bioactive lipid mediators including eicosanoids and lipoxins. The encoded enzyme and its reaction products have been shown to regulate platelet function. Elevated expression of this gene has been observed in pancreatic islets derived from human diabetes patients. Allelic variants in this gene may be associated with susceptibility to toxoplasmosis. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017]