H6PD (NM_004285) Human Tagged ORF Clone

CAT#: RG209890

H6PD (tGFP-tagged) - Human hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD)

Product Images

Circular map for RG209890

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: H6PD
• Synonyms: CORTRD1; G6PDH; GDH; H6PDH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209890 representing NM_004285
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAATATGCTCATAGTGGCGATGTGCTTGGCCCTTCTGGGCTGCCTGCAAGCCCAGGAGCTCCAGG
  GACATGTCTCCATAATCCTGCTGGGAGCAACTGGGGACCTGGCTAAGAAGTACTTATGGCAGGGACTGTT
  CCAGCTGTACCTGGATGAAGCGGGGAGGGGTCACAGTTTTAGCTTCCATGGAGCTGCTCTGACAGCCCCC
  AAGCAGGGTCAAGAGCTCATGGCCAAGGCCCTGGAATCCCTCTCCTGCCCCAAGGACATGGCACCCAGTC
  ACTGTGCAGAGCACAAGGATCAGTTCCTGCAGCTGAGCCAGTACCGCCAACTGAAGACGGCCGAGGACTA
  TCAGGCCCTGAACAAGGACATCGAGGCACAGCTCCAGCACGCAGGCCTCCGGGAGGCTGGCAGGATCTTC
  TACTTCTCAGTGCCACCCTTCGCCTATGAAGACATTGCCCGCAACATCAACAGTAGCTGCCGGCCAGGCC
  CGGGCGCCTGGCTGCGGGTTGTCCTTGAGAAACCCTTTGGCCATGACCACTTCTCAGCCCAGCAGCTGGC
  CACAGAACTCGGGACCTTTTTCCAGGAGGAGGAGATGTACCGGGTGGACCATTACTTAGGCAAGCAGGCT
  GTGGCACAGATCCTGCCTTTCCGAGACCAGAACCGCAAGGCTTTGGACGGCCTCTGGAACCGGCACCATG
  TGGAGCGGGTGGAGATCATCATGAAAGAGACCGTGGATGCCGAAGGCCGCACCAGCTTCTATGAGGAGTA
  CGGTGTCATTCGCGACGTCCTCCAGAACCATCTGACGGAGGTCCTCACCCTCGTGGCCATGGAGCTGCCC
  CACAATGTCAGCAGTGCGGAGGCTGTGCTGCGGCACAAGCTTCAGGTCTTCCAGGCGCTGCGGGGCCTGC
  AGAGGGGCAGTGCCGTCGTGGGCCAGTACCAGTCTTACAGTGAGCAGGTGCGCAGAGAGCTGCAGAAGCC
  AGACAGCTTCCACAGCCTGACGCCGACCTTCGCAGCCGTCCTAGTGCACATTGACAACCTTCGCTGGGAG
  GGCGTGCCTTTCATCCTGATGTCTGGCAAAGCCTTGGACGAGAGAGTGGGCTACGCTCGGATCTTGTTCA
  AGAACCAGGCCTGCTGTGTGCAGAGCGAAAAGCACTGGGCCGCGGCGCAGAGCCAGTGCCTGCCCCGGCA
  GCTCGTCTTCCACATCGGCCATGGCGACCTGGGCAGCCCTGCCGTGCTGGTCAGCAGGAACCTGTTCAGG
  CCCTCCCTGCCCTCCAGCTGGAAGGAAATGGAGGGACCACCTGGGCTCCGCCTTTTCGGCAGCCCTCTGT
  CCGATTACTACGCCTACAGCCCTGTGCAGGAGCGGGACGCCCACTCCGTCCTCTTATCCCATATCTTCCA
  TGGCCGGAAGAATTTCTTCATCACCACAGAGAACTTGCTGGCCTCCTGGAACTTCTGGACCCCTCTGCTG
  GAGAGCCTGGCCCATAAGGCCCCACGCCTCTACCCTGGAGGAGCTGAGAATGGCCGTCTGTTGGACTTTG
  AGTTCAGTAGCGGCCGGTTGTTCTTTTCCCAGCAGCAGCCGGAGCAGCTGGTGCCAGGGCCAGGGCCGGC
  CCCAATGCCCAGTGACTTCCAGGTCCTCAGGGCCAAGTACCGAGAGAGCCCGCTGGTCTCCGCCTGGTCC
  GAGGAGCTGATCTCTAAGCTGGCTAATGACATCGAGGCCACCGCTGTGCGAGCCGTGCGGCGCTTTGGCC
  AGTTCCACCTGGCACTGTCGGGGGGCTCGAGCCCCGTGGCCCTGTTCCAGCAGCTGGCCACGGCGCACTA
  TGGCTTCCCCTGGGCCCACACGCACCTGTGGCTGGTTGACGAGCGCTGCGTCCCACTCTCAGACCCGGAG
  TCCAACTTCCAGGGCCTGCAGGCCCACCTGCTGCAGCACGTCCGGATCCCCTACTACAACATCCACCCCA
  TGCCTGTGCACCTGCAGCAGCGGCTCTGCGCCGAGGAGGACCAGGGCGCCCAGATCTATGCCAGGGAGAT
  CTCAGCCCTGGTGGCCAACAGCAGCTTCGACCTGGTGCTGCTGGGCATGGGTGCCGACGGGCACACAGCC
  TCCCTCTTCCCACAGTCACCCACTGGCCTGGATGGCGAGCAGCTGGTCGTGCTGACCACGAGCCCCTCCC
  AGCCACACCGCCGCATGAGCCTTAGCCTGCCTCTCATCAACCGCGCCAAGAAGGTGGCAGTCCTGGTCAT
  GGGCAGGATGAAGCGTGAGATCACCACGCTGGTGAGCCGGGTGGGCCATGAGCCCAAGAAGTGGCCCATC
  TCGGGTGTCCTGCCGCACTCCGGCCAGCTGGTGTGGTACATGGACTACGACGCCTTCCTGGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209890 representing NM_004285
  Red=Cloning site Green=Tags(s)
  
  MWNMLIVAMCLALLGCLQAQELQGHVSIILLGATGDLAKKYLWQGLFQLYLDEAGRGHSFSFHGAALTAP
  KQGQELMAKALESLSCPKDMAPSHCAEHKDQFLQLSQYRQLKTAEDYQALNKDIEAQLQHAGLREAGRIF
  YFSVPPFAYEDIARNINSSCRPGPGAWLRVVLEKPFGHDHFSAQQLATELGTFFQEEEMYRVDHYLGKQA
  VAQILPFRDQNRKALDGLWNRHHVERVEIIMKETVDAEGRTSFYEEYGVIRDVLQNHLTEVLTLVAMELP
  HNVSSAEAVLRHKLQVFQALRGLQRGSAVVGQYQSYSEQVRRELQKPDSFHSLTPTFAAVLVHIDNLRWE
  GVPFILMSGKALDERVGYARILFKNQACCVQSEKHWAAAQSQCLPRQLVFHIGHGDLGSPAVLVSRNLFR
  PSLPSSWKEMEGPPGLRLFGSPLSDYYAYSPVQERDAHSVLLSHIFHGRKNFFITTENLLASWNFWTPLL
  ESLAHKAPRLYPGGAENGRLLDFEFSSGRLFFSQQQPEQLVPGPGPAPMPSDFQVLRAKYRESPLVSAWS
  EELISKLANDIEATAVRAVRRFGQFHLALSGGSSPVALFQQLATAHYGFPWAHTHLWLVDERCVPLSDPE
  SNFQGLQAHLLQHVRIPYYNIHPMPVHLQQRLCAEEDQGAQIYAREISALVANSSFDLVLLGMGADGHTA
  SLFPQSPTGLDGEQLVVLTTSPSQPHRRMSLSLPLINRAKKVAVLVMGRMKREITTLVSRVGHEPKKWPI
  SGVLPHSGQLVWYMDYDAFLG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004285
• ORF Size: 2373 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004285.3, NP_004276.2
• RefSeq Size: 9117 bp
• RefSeq ORF: 2376 bp
• Locus ID: 9563
• UniProt ID: O95479
• Cytogenetics: 1p36.22
• Domains: G6PD, Glucosamine_iso
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway
• Gene Summary: There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells. [provided by RefSeq, Jul 2008]