H6PD (NM_004285) Human Untagged Clone

CAT#: SC117481

H6PD (untagged)-Human hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: H6PD
• Synonyms: CORTRD1; G6PDH; GDH; H6PDH
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004285 edited
  GGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGACACTATA
  GAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATCGGCACGAGGAGCCTTTTAAC
  TGGCACCCAGGCATGTGGAATATGCTCATAGTGGCGATGTGCTTGGCCCTTCTGGGCTGC
  CTGCAAGCCCAGGAGCTCCAGGGACATGTCTCCATAATCCTGCTGGGAGCAACTGGGGAC
  CTGGCTAAGAAGTACTTATGGCAGGGACTGTTCCAGCTGTACCTGGATGAAGCGGGGAGG
  GGTCACAGTTTTAGCTTCCATGGAGCTGCTCTGACAGCCCCCAAGCAGGGTCAAGAGCTC
  ATGGCCAAGGCCCTGGAATCCCTCTCCTGCCCCAAGGACATGGCACCCAGTCACTGTGCA
  GAGCACAAGGATCAGTTCCTGCAGCTGAGCCAGTACCGCCAACTGAAGACGGCCGAGGAC
  TATCAGGCCCTGAACAAGGACATCGAGGCACAGCTCCAGCACGCAGGCCTCCGGGAGGCT
  GGCAGGATCTTCTACTTCTCAGTGCCACCCTTCGCCTATGAAGACATTGCCCGCAACATC
  AACAGTAGCTGCCGGCCAGGCCCGGGCGCCTGGCTGCGGGTTGTCCTTGAGAAACCCTTT
  GGCCATGACCACTTCTCAGCCCAGCAGCTGGCCACAGAACTCGGGACCTTTTTCCAGGAG
  GAGGAGATGTACCGGGTGGACCATTACTTAGGCAAGCAGGCTGTGGCGCAGATCCTGCCT
  TTCCGAGACCAGAACCGCAAGGCTTTGGACGGCCTCTGGAACCGGCACCATGTGGAGCGG
  GTGGAGATCATCATGAAAGAGACCGTGGATGCTGAAGGCCGCACCAGCTTCTATGAGGAG
  TACGGTGTCATTCGCGACGTCCTCCAGAACCATCTGACGGAGGTCCTCACCCTCGTGGCC
  ATGGAGCTGCCCCACAATGTCAGCAGTGCGGAGGCTGTGCTGCGGCACAAGCTTCAGGTC
  TTCCAGGCGCTGCGGGGCCTGCAGAGGGGCAGTGCCGTCGTGGGCCAGTACCAGTCTTAC
  AGTGAGCAGGTGCGCAGAGAGCTGCAGAAGCCAGACAGCTTCCACAGCCTGACGCCGACC
  TTCGCAGCCGTCCTAGTGCACATTGACAACCTTCGCTGGGAGGGCGTGCCTTTCATCCTG
  ATGTCTGGCAAAGCCTTGGACGAGAGAGTGGGCTACGCTCGGATCTTGTTCAAGAACCAG
  GCCTGCTGTGTGCAGAGCGAAAAGCACTGGGCCGCGGCGCAGAGCCAGTGCCTGCCCCGG
  CAGCTCGTCTTCCACATCGGCCATGGCGACCTGGGCAGCCCTGCCGTGCTGGTCAGCAGG
  AACCTGTTCAGGCCCTCCCTGCCCTCCAGCTGGAAGGAAATGGAGGGACCACCTGGGCTC
  CGCCTTTTCGGCAGCCCTCTGTCCGATTACTACGCCTACAGCCCTGTGCGGGAGCGGGAC
  GCCCACTCCGTCCTCTTATCCCATATCTTCCATGGCCGGAAGAATTTCTTCATCACCACA
  GAGAACTTGCTGGCCTCCTGGAACTTCTGGACCCCTCTGCTGGAGAGCCTGGCCCATAAG
  GCCCCACGCCTCTACCCTGGAGGAGCTGAGAATGGCCGTCTGTTGGACTTTGAGTTCAGT
  AGCGGCCGGTTGTTCTTTTCCCAGCAGCAGCCGGAGCAGCTGGTGCCAGGGCCAGGGCCG
  GCCCCAATGCCCAGTGACTTCCAGGTCCTCAGGGCCAAGTACCGAGAGAGCCCGCTGGTC
  TCCGCCTGGTCCGAGGAGCTGATCTCTAAGCTGGCTAATGACATCGAGGCCACCGCTGTG
  CGAGCCGTGCGGCGCTTTGGCCAGTTCCACCTGGCACTGTCGGGGGGCTCGAGCCCCGTG
  GCCCTGTTCCAGCAGCTGGCCACGGCGCACTATGGCTTCCCCTGGGCCCACACGCACCTG
  TGGCTGGTTGACGAGCGCTGCGTCCCACTCTCAGACCCGGAGTCCAACTTCCAGGGCCTG
  CAGGCCCACCTGCTGCAGCACGTCCGGATCCCCTACTACAACATCCACCCCATGCCTGTG
  CACCTGCAGCAGCGGCTCTGCGCCGAGGAGGACCAGGGCGCCCAGATCTATGCCAGGGAG
  ATCTCAGCCCTGGTGGCCAACAGCAGCTTCGACCTGGTGCTGCTGGGCATGGGTGCCGAC
  GGGCACACAGCCTCCCTCTTCCCACAGTCACCCACTGGCCTGGATGGCGAGCAGCTGGTC
  GTGCTGACCACGAGCCCCTCCCAGCCACACCGCCGCATGAGCCTTAGCCTGCCTCTCATC
  AACCGCGCCAAGAAGGTGGCAGTCCTGGTCATGGGCAGGATGAAGCGTGAGATCACCACG
  CTGGTGAGCCGGGTGGGCCATGAGCCCAAGAAGTGGCCCATCTCGGGTGTCCTGCCGCAC
  TCCGGCCAGCTGGTGTGGTACATGGACTACGACGCCTTCCTGGGATGAGGGCGCCTGTGC
  CCCTTGCCCGCTTCGCTCCTGTGCTTTCCTTCGCCCGTGTCTTCCCTCCCTTCTCGGCCC
  CGCCACCTGCCCAGCGTGCCCTGGCTCTCCAGAACCTTCTATCCCACAGTCAGGCCCCAG
  AGAGGGCAGGACAAGCCTTGTCCCGATGCCTTTGACCGGCAGCTCTGTGTATTGGTGGAT
  AGATGCAGAAACAAGGAAGAAATGGAGTCTGCTCCTGAGAAGCTTCAAATTCAGGCCAGG
  AGAGAAGTCTTAAGAAAAGACCTCCAGCAGTTACACATTCATATCAACCAGCACAACACG
  GGATGGCGCCCAAACTCCGGCGTTCACAAGAGGAGACGTGACGTGGTGGGCTGAGGTTAA
  TCAGGGAAGGTTTCCTGGGGGAGGTGATCCTTGAACTGGCTCCCGGGGAACATTCAGAGC
  ATGATTGGTAGACAGAAGGGTGCAGAGGCGCCCAGGGGAGTACATTGCCCCGTGC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004285 unedited
  CGCCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT
  CATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATCGG
  CACGAGGAGCCTTTTAACTGGCACCCAGGCATGTGGAATATGCTCATAGTGGCGATGTGC
  TTGGCCCTTCTGGGCTGCCTGCAAGCCCAGGAGCTCCAGGGACATGTCTCCATAATCCTG
  CTGGGAGCAACTGGGGACCTGGCTAAGAAGTACTTATGGCAGGGACTGTTCCAGCTGTAC
  CTGGATGAAGCGGGGAGGGGTCACAGTTTTAGCTTCCATGGAGCTGCTCTGACAGCCCCC
  AAGCAGGGTCAAGAGCTCATGGCCAAGGCCCTGGAATCCCTCTCCTGCCCCAAGGACATG
  GCACCCAGTCACTGTGCAGAGCACAAGGATCAGTTCCTGCAGCTGAGCCAGTACCGCCAA
  CTGAAGACGGCCGAGGACTATCAGGCCCTGAACAAGGACATCGAGGCACAGCTCCAGCAC
  GCAGGCCTCCGGGAGGCTGGCAGGATCTTCTACTTCTCAGTGCCACCCTTCGCCTATGAA
  GACATTGCCCGCAACATCAACAGTAGCTGCCGGCCAGGCCCGGGCGCCTGGCTGCGGGTT
  GTCCTTGAGAAACCCTTTGGCCATGACCACTTCTCAGCCCAGCAGCTGGCCACAGAAACT
  CGGACCTTNTTCCAGNAGGAGGAGATGTACCCGGTGGACCATTACTTANNGCAGCAGGCT
  GTGGCGCAGATCCTGCCTTTNCGAGACCAGACCGNCAGGCTNTGNACGGNCTCTGNAACC
  NGCACATGTGNAGCGGNTGGAGNACATCATGAAAGAGACCGTGATGCTGAAGGCGCACAG
  CTCTATGAGAGTACGTGTCATTCCGACGTCTCCAGACATCTGACGAGGTCTNACCTCGTG
  GCTGNACTGCCACATGTCACATGCGGAGCTGGCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004285
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004285.3, NP_004276.2
• RefSeq Size: 9117 bp
• RefSeq ORF: 2376 bp
• Locus ID: 9563
• UniProt ID: O95479
• Cytogenetics: 1p36.22
• Domains: G6PD, Glucosamine_iso
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway
• Gene Summary: There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate 5' most exon compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.