RDH10 (NM_172037) Human Tagged ORF Clone

CAT#: RG208980

RDH10 (tGFP-tagged) - Human retinol dehydrogenase 10 (all-trans) (RDH10)

Product Images

Circular map for RG208980

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RDH10
• Synonyms: SDR16C4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208980 representing NM_172037
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACATCGTGGTGGAGTTCTTCGTGGTCACTTTCAAAGTGCTCTGGGCGTTCGTGCTGGCCGCGGCGC
  GCTGGCTGGTGCGGCCCAAGGAGAAGAGCGTGGCGGGCCAGGTGTGCCTCATCACCGGCGCCGGCAGCGG
  CCTGGGCCGCCTCTTCGCGCTGGAGTTCGCCCGGCGTCGGGCGCTGCTGGTGCTGTGGGACATCAACACG
  CAAAGCAACGAGGAGACGGCTGGCATGGTGCGCCACATCTACCGCGACCTGGAGGCGGCCGACGCCGCTG
  CGCTGCAAGCTGGGAATGGTGAGGAAGAAATTCTGCCCCACTGTAACTTGCAGGTTTTTACCTACACCTG
  TGACGTGGGGAAGAGGGAGAACGTCTACCTGACGGCTGAAAGAGTCCGCAAGGAGGTTGGCGAAGTCTCA
  GTCCTGGTCAATAATGCTGGTGTGGTCTCTGGGCATCACCTTCTGGAATGTCCTGATGAGCTCATTGAGA
  GAACCATGATGGTCAATTGCCATGCACACTTCTGGACCACTAAGGCTTTTCTTCCTACGATGCTGGAGAT
  TAATCATGGTCATATTGTGACAGTTGCAAGTTCCTTGGGATTGTTCAGTACTGCCGGAGTTGAGGATTAC
  TGTGCCAGTAAATTTGGAGTTGTGGGTTTTCATGAATCCCTGAGCCATGAACTAAAGGCTGCTGAAAAGG
  ATGGAATTAAAACAACCTTGGTTTGCCCTTATCTTGTAGACACTGGCATGTTCAGAGGCTGCCGAATCAG
  GAAAGAAATTGAGCCTTTTCTGCCACCTCTGAAGCCTGATTACTGTGTGAAGCAGGCCATGAAGGCCATC
  CTCACTGACCAGCCCATGATCTGCACTCCCCGCCTCATGTACATCGTGACCTTCATGAAGAGCATCCTAC
  CATTTGAAGCAGTTGTGTGCATGTATCGGTTCCTAGGAGCGGACAAGTGTATGTACCCCTTTATTGCTCA
  AAGAAAGCAAGCCACAAACAATAATGAAGCAAAAAATGGAATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208980 representing NM_172037
  Red=Cloning site Green=Tags(s)
  
  MNIVVEFFVVTFKVLWAFVLAAARWLVRPKEKSVAGQVCLITGAGSGLGRLFALEFARRRALLVLWDINT
  QSNEETAGMVRHIYRDLEAADAAALQAGNGEEEILPHCNLQVFTYTCDVGKRENVYLTAERVRKEVGEVS
  VLVNNAGVVSGHHLLECPDELIERTMMVNCHAHFWTTKAFLPTMLEINHGHIVTVASSLGLFSTAGVEDY
  CASKFGVVGFHESLSHELKAAEKDGIKTTLVCPYLVDTGMFRGCRIRKEIEPFLPPLKPDYCVKQAMKAI
  LTDQPMICTPRLMYIVTFMKSILPFEAVVCMYRFLGADKCMYPFIAQRKQATNNNEAKNGI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172037
• ORF Size: 1023 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172037.5
• RefSeq Size: 2751 bp
• RefSeq ORF: 1026 bp
• Locus ID: 157506
• UniProt ID: Q8IZV5
• Cytogenetics: 8q21.11
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Retinol metabolism
• Gene Summary: This gene encodes a retinol dehydrogenase, which converts all-trans-retinol to all-trans-retinal, with preference for NADP as a cofactor. Studies in mice suggest that this protein is essential for synthesis of embryonic retinoic acid and is required for limb, craniofacial, and organ development. [provided by RefSeq, Dec 2011]