SMAD3 (NM_005902) Human Tagged ORF Clone
SMAD3 (GFP-tagged) - Human SMAD family member 3 (SMAD3), transcript variant 1
|Product Name||SMAD3 (NM_005902) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3|
|E. coli Selection||Ampicillin (100 ug/mL)|
>RG208749 representing NM_005902
Red=Cloning site Blue=ORF Green=Tags(s)
ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG208749 representing NM_005902
Red=Cloning site Green=Tags(s)
TRTRPLE - GFP Tag - V
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1275 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||6256 bp|
|RefSeq ORF||1278 bp|
|Domains||DWB, DWA, MH1|
|Protein Families||Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors|
|Protein Pathways||Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway|
|Gene Summary||The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. It also functions as a tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3. [provided by RefSeq, Nov 2019]|
|cDNA Clone Resources|
|SC321871||SMAD3 (untagged)-Human SMAD family member 3 (SMAD3), transcript variant 1||
|RC208749||SMAD3 (Myc-DDK-tagged)-Human SMAD family member 3 (SMAD3), transcript variant 1||
|RC208749L1||Lenti ORF clone of Human SMAD family member 3 (SMAD3), transcript variant 1, Myc-DDK-tagged||
|RC208749L2||Lenti ORF clone of Human SMAD family member 3 (SMAD3), transcript variant 1, mGFP tagged||
|RC208749L3||Lenti ORF clone of Human SMAD family member 3 (SMAD3), transcript variant 1, Myc-DDK-tagged||
|RC208749L4||Lenti ORF clone of Human SMAD family member 3 (SMAD3), transcript variant 1, mGFP tagged||