FPGT (NM_003838) Human Tagged ORF Clone

CAT#: RG207119

FPGT (tGFP-tagged) - Human fucose-1-phosphate guanylyltransferase (FPGT), transcript variant 1

Product Images

Circular map for RG207119

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FPGT
• Synonyms: GFPP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207119 representing NM_003838
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTGCTAGGGACCCTCCGGAAGTATCGCTGCGAGAAGCCACCCAGCGAAAATTGCGGAGGTTTT
  CCGAGCTAAGAGGCAAACTTGTAGCACGTGGAGAATTCTGGGACATAGTTGCAATAACAGCGGCTGATGA
  AAAACAGGAACTTGCTTACAACCAACAGCTGTCAGAAAAGCTGAAAAGAAAGGAGTTACCCCTTGGAGTT
  CAATATCACGTTTTTGTAGATCCTGCTGGAGCCAAAATTGGAAATGGAGGATCAACACTTTGTGCCCTTC
  AATGTTTGGAAAAGCTATATGGAGATAAATGGAATTCTTTTACCATCTTATTAATTCACTCTGGTGGCTA
  CAGTCAACGACTTCCAAATGCAAGTGCTCTGGGAAAAATTTTCACTGCTTTACCTCTTGGTAACCCCATT
  TATCAGATGCTAGAATTAAAACTAGCCATGTACATTGATTTCCCCTTAAATATGAATCCTGGAATTCTGG
  TTACCTGTGCAGATGATATTGAACTTTATAGTATTGGAGAATTTGAGTTTATTAGGTTTGACAAACCTGG
  CTTTACTGCTTTAGCTCATCCTTCTAGTTTGACGATAGGTACCACACATGGAGTATTTGTCTTAGATCCT
  TTTGATGATTTAAAACATAGAGACCTTGAATACAGGTCTTGCCATCGTTTCCTTCATAAGCCCAGCATAG
  AAAAGATGTATCAGTTTAATGCTGTGTGTAGACCTGGAAATTTTTGTCAACAGGACTTTGCTGGGGGTGA
  CATTGCCGATCTTAAATTAGACTCTGACTATGTCTACACAGATAGCCTATTTTATATGGATCATAAATCA
  GCAAAAATGTTACTTGCTTTTTATGAAAAAATAGGCACACTGAGCTGTGAAATAGATGCCTATGGTGACT
  TTCTGCAGGCTTTGGGACCTGGAGCAACTGTGGAGTACACCAGAAACACATCAAATGTCATTAAAGAAGA
  GTCAGAGTTGGTAGAAATGAGGCAGAGAATATTTCATCTTCTTAAAGGAACATCACTAAATGTTGTTGTT
  CTTAATAACTCCAAATTTTATCACATTGGAACAACCGAAGAATATTTGTTTTACTTTACCTCAGATAACA
  GTTTAAAGTCAGAGCTCGGCTTACAGTCCATAACTTTTAGTATCTTTCCAGATATACCAGAATGCTCTGG
  CAAAACATCCTGTATCATTCAAAGCATACTGGATTCAAGATGTTCTGTGGCACCTGGCTCAGTTGTGGAG
  TATTCCAGATTGGGGCCTGATGTTTCAGTTGGGGAAAACTGCATTATTAGTGGTTCTTACATCCTAACAA
  AAGCTGCCCTCCCCGCACATTCTTTTGTATGTTCCTTAAGCTTAAAGATGAATAGATGCTTAAAGTATGC
  AACTATGGCATTTGGAGTGCAAGACAACTTGAAAAAGAGTGTGAAAACATTGTCAGATATAAAGTTACTT
  CAATTCTTTGGAGTCTGTTTCCTGTCATGCTTAGATGTTTGGAATCTTAAAGTTACAGAGGAACTGTTCT
  CTGGTAACAAGACATGTCTGAGTTTGTGGACTGCACGCATTTTCCCAGTTTGTTCTTCTTTGAGTGACTC
  AGTTATAACATCCCTAAAGATGTTAAATGCTGTTAAGAACAAGTCAGCATTCAGCCTGAATAGCTATAAG
  TTGCTGTCCATTGAAGAAATGCTTATCTACAAAGATGTAGAAGATATGATAACTTACAGGGAACAAATTT
  TTCTAGAAATCAGTTTAAAAAGCAGTTTGATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207119 representing NM_003838
  Red=Cloning site Green=Tags(s)
  
  MAAARDPPEVSLREATQRKLRRFSELRGKLVARGEFWDIVAITAADEKQELAYNQQLSEKLKRKELPLGV
  QYHVFVDPAGAKIGNGGSTLCALQCLEKLYGDKWNSFTILLIHSGGYSQRLPNASALGKIFTALPLGNPI
  YQMLELKLAMYIDFPLNMNPGILVTCADDIELYSIGEFEFIRFDKPGFTALAHPSSLTIGTTHGVFVLDP
  FDDLKHRDLEYRSCHRFLHKPSIEKMYQFNAVCRPGNFCQQDFAGGDIADLKLDSDYVYTDSLFYMDHKS
  AKMLLAFYEKIGTLSCEIDAYGDFLQALGPGATVEYTRNTSNVIKEESELVEMRQRIFHLLKGTSLNVVV
  LNNSKFYHIGTTEEYLFYFTSDNSLKSELGLQSITFSIFPDIPECSGKTSCIIQSILDSRCSVAPGSVVE
  YSRLGPDVSVGENCIISGSYILTKAALPAHSFVCSLSLKMNRCLKYATMAFGVQDNLKKSVKTLSDIKLL
  QFFGVCFLSCLDVWNLKVTEELFSGNKTCLSLWTARIFPVCSSLSDSVITSLKMLNAVKNKSAFSLNSYK
  LLSIEEMLIYKDVEDMITYREQIFLEISLKSSLM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003838
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003838.2, NP_003829.2
• RefSeq Size: 3426 bp
• RefSeq ORF: 1785 bp
• Locus ID: 8790
• UniProt ID: O14772
• Cytogenetics: 1p31.1
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
• Gene Summary: L-fucose is a key sugar in glycoproteins and other complex carbohydrates since it may be involved in many of the functional roles of these macromolecules, such as in cell-cell recognition. The fucosyl donor for these fucosylated oligosaccharides is GDP-beta-L-fucose. There are two alternate pathways for the biosynthesis of GDP-fucose; the major pathway converts GDP-alpha-D-mannose to GDP-beta-L-fucose. The protein encoded by this gene participates in an alternate pathway that is present in certain mammalian tissues, such as liver and kidney, and appears to function as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids. This pathway involves the phosphorylation of L-fucose to form beta-L-fucose-1-phosphate, and then condensation of the beta-L-fucose-1-phosphate with GTP by fucose-1-phosphate guanylyltransferase to form GDP-beta-L-fucose. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream TNNI3 interacting kinase (TNNI3K) gene. [provided by RefSeq, Dec 2010]