KCTD12 (NM_138444) Human Tagged ORF Clone

CAT#: RG204577

KCTD12 (tGFP-tagged) - Human potassium channel tetramerisation domain containing 12 (KCTD12)

Product Images

Circular map for RG204577

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KCTD12
• Synonyms: C13orf2; PFET1; PFETIN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204577 representing NM_138444
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGGCGGACAGCACACGTGGATTACCCAACGGGGGCGGCGGCGGGGGCGGCAGTGGCTCCTCGT
  CGTCCTCCGCGGAGCCACCGCTCTTCCCCGACATCGTGGAGCTGAACGTGGGGGGCCAGGTGTACGTGAC
  CCGGCGCTGCACGGTGGTGTCGGTGCCCGACTCGCTGCTCTGGCGCATGTTCACGCAGCAGCAGCCGCAG
  GAGCTGGCCCGGGACAGCAAAGGCCGCTTCTTTCTGGACCGGGACGGCTTCCTCTTCCGCTACATCCTGG
  ATTACCTGCGGGACTTGCAGCTCGTGCTGCCCGACTACTTCCCCGAGCGCAGCCGGCTGCAGCGCGAGGC
  CGAGTACTTCGAGCTGCCAGAGCTCGTGCGCCGCCTCGGGGCGCCCCAGCAGCCCGGCCCGGGGCCGCCG
  CCCTCGCGGCGCGGGGTGCACAAGGAGGGCTCGCTGGGTGACGAGCTGCTGCCGCTTGGCTACTCGGAGC
  CCGAACAGCAGGAGGGCGCCTCTGCCGGGGCGCCGTCGCCCACGCTGGAGCTGGCTAGCCGCAGTCCGTC
  CGGGGGCGCGGCGGGCCCGCTGCTCACGCCGTCCCAGTCGCTGGACGGCAGCCGGCGCTCGGGCTACATC
  ACCATCGGCTACCGCGGCTCCTACACCATCGGGCGGGACGCGCAGGCGGACGCCAAGTTCCGGCGAGTGG
  CGCGCATCACCGTTTGCGGAAAGACGTCGCTGGCCAAGGAGGTGTTTGGGGACACCCTGAACGAAAGCCG
  GGACCCCGACCGTCCCCCGGAGCGCTACACCTCGCGCTATTACCTCAAGTTCAACTTCCTGGAGCAGGCC
  TTCGACAAGCTGTCCGAGTCGGGCTTCCACATGGTGGCGTGCAGCTCCACGGGCACCTGCGCCTTTGCCA
  GCAGCACCGACCAGAGCGAGGACAAGATCTGGACCAGCTACACCGAGTACGTCTTCTGCAGGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204577 representing NM_138444
  Red=Cloning site Green=Tags(s)
  
  MALADSTRGLPNGGGGGGGSGSSSSSAEPPLFPDIVELNVGGQVYVTRRCTVVSVPDSLLWRMFTQQQPQ
  ELARDSKGRFFLDRDGFLFRYILDYLRDLQLVLPDYFPERSRLQREAEYFELPELVRRLGAPQQPGPGPP
  PSRRGVHKEGSLGDELLPLGYSEPEQQEGASAGAPSPTLELASRSPSGGAAGPLLTPSQSLDGSRRSGYI
  TIGYRGSYTIGRDAQADAKFRRVARITVCGKTSLAKEVFGDTLNESRDPDRPPERYTSRYYLKFNFLEQA
  FDKLSESGFHMVACSSTGTCAFASSTDQSEDKIWTSYTEYVFCRE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138444
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138444.4
• RefSeq Size: 6255 bp
• RefSeq ORF: 978 bp
• Locus ID: 115207
• UniProt ID: Q96CX2
• Cytogenetics: 13q22.3
• Domains: BTB, K_tetra
• Protein Families: Ion Channels: Other
• Gene Summary: Auxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization (By similarity).[UniProtKB/Swiss-Prot Function]